Study of the Structure and Antimicrobial Activity of Ca-Deficient Ceramics on Chlorhexidine Nanoclay Substrate

Pazourková, Lenka; Reli, Martin; Hundáková, Marianna; Pazdziora, Erich; Predoi, Daniela; Martynková, Gražyna Simha; Lafdi, Khalid

doi:10.3390/ma12182996

Cited by 13 publications

(10 citation statements)

References 35 publications

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“…Considering that endodontic infections can be polymicrobial, further studies should evaluate multispecies biofilms. Furthermore, general oral environment can be altered after prosthodontic [ 35 ], orthodontic [ 36 ], or implantologic [ 37 ] treatments. In these cases, the E. faecalis biofilm could be altered or modified.…”

Section: Discussionmentioning

confidence: 99%

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

et al. 2020

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Biofilm communities are tolerant to antimicrobials and difficult to eradicate. This study aimed to investigate the effect of melittin, an antimicrobial peptide, either alone or in combination with deoxyribonuclease (DNase), an inhibitor of extracellular deoxyribonucleic acid (eDNA), against Enterococcus faecalis (E. faecalis) biofilms, and biofilm susceptibility to sodium hypochlorite (NaOCl). Biofilms of E. faecalis were developed in root canals of bovine teeth. The biofilms were treated with distilled water (control), melittin, DNase, or DNase+melittin. The antibiofilm effects of the treatments were analyzed using colony forming unit (CFU) assay, crystal violet staining, confocal laser scanning microscopy (CLSM), and field emission scanning electron microscope (FE-SEM). The susceptibility of DNase+melittin-treated biofilms to NaOCl (0%, 2.5% and 5%) was investigated by the CFU assay. The data were statistically analyzed using one-way analysis of variance, followed by Tukey’s test. A p-value of <0.05 was considered significant. Specimens treated with DNase+melittin showed a more significant decrease in the CFUs, eDNA level, and biofilm formation rate than those treated only with melittin or DNase (p < 0.05). CLSM analysis showed DNase+melittin treatment significantly reduced the volume of biofilms and extracellular polymeric substance compared to either treatment alone (p < 0.05). FE-SEM images showed a high degree of biofilm disruption in specimens that received DNase+melittin. 2.5% NaOCl in specimens pretreated with DNase+melittin showed higher antibacterial activity than those treated only with 5% NaOCl (p < 0.05). This study highlighted that DNase improved the antibiofilm effects of melittin. Moreover, DNase+melittin treatment increased the susceptibility of biofilms to NaOCl. Thus, the complex could be a clinical strategy for safer use of NaOCl by reducing the concentration.

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Section: Discussionmentioning

confidence: 99%

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

et al. 2020

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“…Bioceramic materials, such as zirconia, alumina or groups of hydroxyapatites (HAp) are widely used in medical applications, especially in orthopedics and dental prosthetics [1][2][3]. The bioceramics used in medicine (e.g., for hips, knees and teeth replacements) may be nonporous and almost bioinert (e.g., zirconia or alumina), bioactive (e.g., dense HAp or glass-ceramic), resorbable (e.g., tricalcium phosphate) and porous for tissue ingrowth (e.g., porous Hap or HAp-coated porous metals) [4,5].…”

Section: Introductionmentioning

confidence: 99%

Alkali-Treated Alumina and Zirconia Powders Decorated with Hydroxyapatite for Prospective Biomedical Applications

et al. 2022

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The alumina and zirconia surfaces were pretreated with chemical etching using alkaline mixtures of ammonia, hydrogen peroxide and sodium hydroxide, and followed with application of the powder layer of Ca-deficient hydroxyapatite (CDH). The influence of etching bath conditions time and concentration on surface development, chemical composition and morphology of medicinal ceramic powders were studied. The following analyses were performed: morphology (scanning electron microscopy), phase composition (X-ray diffraction analysis), changes in binding interactions and chemical composition (FT-Infrared and Energy dispersive spectroscopies). Both types of etchants did not expose the original phase composition changes or newly created phases for both types of ceramics. Subsequent decoration of the surface with hydroxyapatite revealed differences in the morphological appearance of the layer on both ceramic surfaces. The treated zirconia surface accepted CDH as a flowing layer on the surface, while the alumina was decorated with individual CDH aggregates. The goal of this study was to focus further on the ceramic fillers for polymer-ceramic composites used as a biomaterial in dental prosthetics.

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“…Important problems are further associated with inflammation caused by pathogenic microorganisms, most often Candida albicans, Typhylococcus aureus group [12], Aggregatibacter actinomycetemcomitans [13], and Eikenella corrodens. It is assumed that over 65% of all human infections have been estimated to be biofilm-related [14][15][16]. Bacterial and fungal inflammations, and their related issues, are the most noticeable in metal biomaterials and alloys [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Simple Approach to Medical Grade Alumina and Zirconia Ceramics Surface Alteration via Acid Etching Treatment

et al. 2021

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In order for bioceramics to be further used in composites and their applications, it is important to change the surface so that the inert material is ready to interact with another material. Medical grade alumina and zirconia ceramic powders have been chemically etched with three selected acidic mixtures. Powder samples were taken for characterization, which was the key to evaluating a successful surface change. Changes in morphology, together with chemical composition, were studied using scanning electron microscopy, phase composition using X-ray diffraction methods, and nitrogen adsorption/desorption isotherms are used to evaluate specific surface area and porosity. The application of HF negatively affected the morphology of the material and caused agglomeration. The most effective modification of ceramic powders was the application of a piranha solution to obtain a new surface and a satisfactory degree of agglomeration. The prepared micro-roughness of the etched ceramic would provide an improved surface of the material either for its next step of incorporation into the selected matrix or to directly aid in the attachment and proliferation of osteoblast cells.

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Study of the Structure and Antimicrobial Activity of Ca-Deficient Ceramics on Chlorhexidine Nanoclay Substrate

Cited by 13 publications

References 35 publications

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

Combined Effect of Melittin and DNase on Enterococcus faecalis Biofilms and Its Susceptibility to Sodium Hypochlorite

Alkali-Treated Alumina and Zirconia Powders Decorated with Hydroxyapatite for Prospective Biomedical Applications

Simple Approach to Medical Grade Alumina and Zirconia Ceramics Surface Alteration via Acid Etching Treatment

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