Study of the KIR gene profiles and analysis of the phylogenetic relationships of Rajbanshi population of West Bengal, India

Study of genetic diversity of KIR and TLR in the Rabhas, an endogamous primitive tribe of India

“…Moreover, genetic distance measures also showed the least distance of the Rabhas from the Rajbanshis in comparison to other Indian populations. An earlier study by Guha et al (2013) [11] suggested that the Indo-European speaking Rajbanshis from the northern part of Bengal has Tibeto-Burman influence in spite of having an Indian origin. Thus, the genetic proximity of the Rabhas with the Rajbanshis clearly indicates the inclination of the Rabhas toward mongoloid ethnicity.…”

“…The frequency data of KIR genes and genotypes in populations to be compared to the Bengalis were extracted from the following publications and from the 'http://www.allelefrequencies.net' database [13] as follows: Indian Rajbanshis [11], South Indian Paravar, Kanikar and Mollukurumba [14], Finnish, French Caucasian, Senegal African, Guadeloupe Caribbean, and Reunion, a population from Indian Ocean origin [15], North Indian [16], Cook Island, Samoan, Tokelau, Tongan [17], Mestizo, Huichol, Purepecha, Tarahumara [18], Amazonian Amerindian [19], Wichis and Chiriguanos [20], Northern Irish [21], Basque population [22], Eastern Mainland Chinese [23], Chinese Han [24], Korean [25], Japanese [26], Warao, Bari, Yucpa [27], Vietnamese and Australian Aborigine [28], American Caucasian, Hispanic, African American [29], Thai, British Caucasian, Palestinian [30], Australian Caucasian [31], New York Caucasian [32], Greeks [33], Afro-Caribbean, Trinidad Asian, Pakistani [34], Chinese, Malay and Indian in Singapore [35], Indian Parsis and Maharashtrian [36], Tibetans [37] and Iranian Arabs and Persians [38].…”

“…The KIR genes and genotypic frequencies of the reference populations were also extracted from the 'http://www.allelefrequencies.net' database [13] and Guha et al 2013 [11].…”

“…The extracted DNAs were then amplified using SSP-PCR reactions for detecting 16 KIR genes (KIR2DL1-5, KIR2DS1-5, 3DL1-3, 3DS1, 2DP1 and 3DP1). The KIR primer sequences were similar to that of Guha et al (2013) [11] which were generously provided by R. Rajalingam on enquiry. The PCR reactions were performed according to Guha et al (2013) [11].…”

“…The KIR primer sequences were similar to that of Guha et al (2013) [11] which were generously provided by R. Rajalingam on enquiry. The PCR reactions were performed according to Guha et al (2013) [11]. We have also genotyped 5 TLR genes (TLR1-TLR5) in the Rabha population.…”