Study of the Electrophoretic Behavior of Cephalosporins by Capillary Zone Electrophoresis

Hancu, Gabriel; Sasebeşi, Adina; Rusu, Aura; Kelemen, Hajnal; Ciurba, Adriana

doi:10.15171/apb.2015.031

Cited by 21 publications

(13 citation statements)

References 15 publications

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“…1, the differences in migration behavior between these peptides were insufficient to separate these peptides using this system. Unlike CZE, MEKC can be used to separate both charged and neutral analytes because hydrophobicity is used as an additional differentiating parameter [20,21]. Thus, MEKC provided an effective means to analyze peptide drugs and related impurities.…”

Section: Methods Development and Optimizationmentioning

confidence: 99%

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Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

et al. 2016

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An MEKC method for the analysis of goserelin and related substances has been developed using a combination of additives including CTAB, β-CD, and sodium hexanesulfonate. For this assay, the running buffer (pH and additives) and separation conditions (voltage and temperature) were optimized. The optimized system was the following: 200 mM 6-aminocaproic acid buffer (pH 4.2) supplemented with 175 mM CTAB, 3.0% w/v β-CD, and 20 mM sodium hexanesulfonate; the voltage was 10 kV in reverse polarity mode, the temperature was 20°C, and UV detection was measured at 220 nm. The method was qualified by evaluating the specificity, precision, linearity, accuracy, LOD, and LOQ. According to validation experiments, the optimized method was specific, accurate, and repeatable and satisfied the requirements for the analysis of goserelin and related substances. Compared with the RP-HPLC method, the MEKC method better solved the problem of overlapping impurity signals, and the migration time required was shorter. This method can be used for quality control and for the analysis of goserelin and its related substances.

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Section: Methods Development and Optimizationmentioning

confidence: 99%

“…The separation principle of MEKC is based on differences in the combination of charge-to-mass ratio, hydrophobicity, and charge interactions at the surface of micelles [20,21]. The main objective of this study was to develop an MEKC method to separate goserelin and its main related substances.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

et al. 2016

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“…Moreover, when M-␤-CD was used as a CS, the complexation constants were higher for 10-hydroxywarfarin than for warfarin, and therefore, the former exerts stronger affinity to M-␤-CD and stronger stereoselective effect. RAMEB was used in CE as a CS for the enantioseparation of several analytes, such as omeprazole, amlodipine, and ofloxacin [51][52][53][54]. Briefly, Hancu et al [51] studied the chiral separation of omeprazole by using several neutral and negatively charged CDs.…”

Section: Neutral CD Derivativesmentioning

confidence: 99%

“…After method validation, chiral analysis of omeprazole in different pharmaceutical preparations was performed. The same research group investigated the chiral separation of amlodipine by using neutral and negatively charged CDs [52]. Among all the examined CDs, RAMEB was selected as the optimum since it provided the best R S (2.48) at the shortest analysis time (Ͻ6 min).…”

Section: Neutral CD Derivativesmentioning

confidence: 99%

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Stavrou

Agathokleous

Kapnissi‐Christodoulou

2017

Electrophoresis

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This report, which is a sequence of a series of reviews, records the most important chiral selectors (CSs) applied in CE. It highlights the CSs that were used during the period 2014 to mid-2016. In this review, method developments, validations, and pharmaceutical along with biomedical applications are presented. The different CSs include CDs, antibiotics, cyclofructants, linear and branched oligo- and polysaccharides, and polymeric surfactants. In addition, the advantages of these CSs, along with their chiral recognition mechanisms, and their performance, are discussed.

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“…The classic CZE method is not suited for the separation of alkaloids because of their similarities in charge/mass ratios and limited solubility. MEKC extends the applicability of CE to neutral analytes [17]. In the last decade, indeed, CE methods for the determination of caffeine and other alkaloids have been reported [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Determination of alkaloids in onion nectar by micellar electrokinetic chromatography

et al. 2016

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Nectar is the most important floral reward offered by plants to insects. Minor components such as alkaloid compounds in nectar affect bee foraging, with great influence in seed production. CE is an advantageous tool for the analysis of unexplored samples such as onion nectar due to the limited amounts of samples. Considering the importance of these compounds, a simultaneous determination of nicotine, theophylline, theobromine, caffeine, harmaline, piperine in onion nectar by MEKC-UV is herein reported. The extraction of alkaloid compounds in nectar was performed by SPE using a homemade miniaturized column (C18 ). Effects of several important factors affecting extraction efficiency as well as electrophoretic performance were investigated to acquire optimum conditions. Under the proposed conditions, the analytes can be separated within 15 min in a 50 cm effective length capillary (75 μm id) at a separation voltage of 20 kV in 20 mmol/L sodium tretraborate, 100 mmol/L SDS. The amount of sample requirement was reduced up to 2000 times, when compared to traditional methods, reaching limits of detection as low as 0.0153 ng/L. For the first time, this study demonstrates that there are marked qualitative and quantitative differences in nectar alkaloids between open pollinated and male sterile lines (MSLs) and also within MSLs.

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Study of the Electrophoretic Behavior of Cephalosporins by Capillary Zone Electrophoresis

Cited by 21 publications

References 15 publications

Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Chiral selectors in CE: Recent development and applications (mid‐2014 to mid‐2016)

Determination of alkaloids in onion nectar by micellar electrokinetic chromatography

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