Study of the DNA hybridization transduction behavior of a quinone-containing electroactive polymer by cyclic voltammetry and electrochemical impedance spectroscopy

Effects of Ionic Strength and Probe DNA Length on the Electrochemical Impedance Spectroscopic Response of Biosensors

“…Some methods use a redox marker to detect the hybridization signal [19][20]. An advantage of this technique is that oligonucleotide labeling is not required to detect DNA, the success of biosensors that are based on label-free impedance sensing has been widely demonstrated [21][22][23].…”

Organic Bioelectronics

“…The impedance characteristics of the electrolyte bulk or over the double layer at the electrode/electrolyte interface also predict the current level that passes through the sensor cell. Benoit Piro and Minh Chao Pham, at Université Paris 7-Denis Diderot in France, developed a bifunctional amperometric sensor [42] polymer consisting of a quinine-containing monomer (JUG), [43,44] serving as the ion-to-electron transducer, as well as a carboxylic site (JUGA) for immobilization of oligonucleotides (Fig. 4b).…”

Immobilization of Biomolecules by Electropolymerized Films

“…For instance, an electrochemical DNA sensor was elaborated from the following copolymeric film: poly(5-hydroxy-1,4-naphthoquinone-co-5 hydroxy-3-thioacetic acid-1,4-naphthoquinone), the carboxylic and quinone entities being used for the chemical grafting and the transduction of the hybridization event, respectively. 40 With the aim of developing a "reagentless approach" to biomolecule grafting, the electrochemical polymerization of thiophene, pyrrole, and dicarbazole derivatives functionalized by easy leaving groups such as N -hydroxysuccinimide, N -hydroxyphtalimide, or pentafluorophenyl esters has led to attractive precursor polymers. 19,41 The latter were efficiently applied to the elaboration of enzyme electrodes and electrochemical immunosensors and DNA sensors.…”