Study of the difference of high and low metastasis cell line's gene expression map and metastasis-related genes of adenoid cystic carcinoma

Zhu, Nianyong; Yang, Jielin; Wang, YingMing; Guan, Xin‐Yuan

doi:10.1038/emm.2003.32

Cited by 5 publications

(1 citation statement)

References 9 publications

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“…Nevertheless, it has been reported that FHC level was relatively low in the high metastatic tumor lines compared with the low metastatic lines, which exhibited better differentiated phenotype in bladder tumor model (29) and adenoid cystic carcinoma (30). Consistent with these reports, in vivo evidence of esophageal carcinoma showed that FHC levels dropped greatly in advanced-stage tumors, especially in scattered and invasive tumor cells compared with normal tissue and early-stage tumors.…”

Section: Discussionmentioning

confidence: 52%

Ferritin Heavy Chain–Mediated Iron Homeostasis and Subsequent Increased Reactive Oxygen Species Production Are Essential for Epithelial-Mesenchymal Transition

et al. 2009

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The epithelial-mesenchymal transition (EMT) plays a critical role in tumor progression. To obtain a broad view of the molecules involved in EMT, we carried out a comparative proteomic analysis of transforming growth factor-B1 (TGF-B1)-induced EMT in AML-12 murine hepatocytes. A total of 36 proteins with significant alterations in abundance were identified. Among these proteins, ferritin heavy chain (FHC), a cellular iron storage protein, was characterized as a novel modulator in TGF-B1-induced EMT. In response to TGF-B1, there was a dramatic decrease in the FHC levels, which caused iron release from FHC and, therefore, increased the intracellular labile iron pool (LIP). Abolishing the increase in LIP blocked TGF-B1-induced EMT. In addition, increased LIP levels promoted the production of reactive oxygen species (ROS), which in turn activated p38 mitogen-activated protein kinase. The elimination of ROS inhibited EMT, whereas H 2 O 2 treatment rescued TGF-B1-induced EMT in cells in which the LIP increase was abrogated. Overexpression of exogenous FHC attenuated the increases in LIP and ROS production, leading to a suppression of EMT. We also showed that TGF-B1-mediated down-regulation of FHC occurs via 3 ¶ untranslated regiondependent repression of the translation of FHC mRNA. Moreover, we found that FHC down-regulation is an event that occurs between the early and highly invasive advanced stages in esophageal adenocarcinoma and that depletion of LIP or ROS suppresses the migration of tumor cells. Our data show that cellular iron homeostasis regulated by FHC plays a critical role in TGF-B1-induced EMT. [Cancer Res 2009;69(13):5340-8]

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Section: Discussionmentioning

confidence: 52%

Ferritin Heavy Chain–Mediated Iron Homeostasis and Subsequent Increased Reactive Oxygen Species Production Are Essential for Epithelial-Mesenchymal Transition

et al. 2009

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Protein and mRNA characterization in high and low metastasis adenoid cystic carcinoma cell lines

et al. 2005

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Metastasis and invasion, the important characteristics of malignant tumors, are closely associated with a series of changes in the expression of genes and proteins. In this study, we compare mRNA and protein expression in high and low metastasis adenoid cystic carcinoma cell lines by mRNA suppression subtractive hybridization and two-dimensional electrophoresis combined with peptide mass fingerprint analysis. 34 differentially expressed genes were obtained using suppression subtractive hybridization experiments including 6 highly expressed gene sequences in the high metastasis cell line, and 28 in the low metastasis cell line. RNA dot blot hybridization further confirmed the results after excluding false positives. For protein analysis, ten significantly different protein spots were detected using two-dimensional gel electrophoresis technique combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI- TOF-MS). The results then compare with the SWISS PROT database. These results suggest that high tumor metastasis of adenoid cystic carcinoma is associated with multiple genes whose function include angiogenesis, protein synthesis, signal transduction, modulation of cell cycle, molecular chaperones, and immune co-stimulating molecule. Moreover, the results of the phenotypic function-related expression mapping analysis at the mRNA and protein level revealed obvious complementarities, providing important clues for further study of the molecular mechanism of metastasis, metastasis control and possible targets for cancer gene therapy.

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One-dimensional microfluidic beads array for multiple mRNAs expression detection

Wen

Yang

Wang

et al. 2007

Biosensors and Bioelectronics

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Study of the difference of high and low metastasis cell line's gene expression map and metastasis-related genes of adenoid cystic carcinoma

Cited by 5 publications

References 9 publications

Ferritin Heavy Chain–Mediated Iron Homeostasis and Subsequent Increased Reactive Oxygen Species Production Are Essential for Epithelial-Mesenchymal Transition

Ferritin Heavy Chain–Mediated Iron Homeostasis and Subsequent Increased Reactive Oxygen Species Production Are Essential for Epithelial-Mesenchymal Transition

Protein and mRNA characterization in high and low metastasis adenoid cystic carcinoma cell lines

One-dimensional microfluidic beads array for multiple mRNAs expression detection

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