Protein and mRNA characterization in high and low metastasis adenoid cystic carcinoma cell lines

Abstract

Metastasis and invasion, the important characteristics of malignant tumors, are closely associated with a series of changes in the expression of genes and proteins. In this study, we compare mRNA and protein expression in high and low metastasis adenoid cystic carcinoma cell lines by mRNA suppression subtractive hybridization and two-dimensional electrophoresis combined with peptide mass fingerprint analysis. 34 differentially expressed genes were obtained using suppression subtractive hybridization experiments including 6 highly expressed gene sequences in the high metastasis cell line, and 28 in the low metastasis cell line. RNA dot blot hybridization further confirmed the results after excluding false positives. For protein analysis, ten significantly different protein spots were detected using two-dimensional gel electrophoresis technique combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI- TOF-MS). The results then compare with the SWISS PROT database. These results suggest that high tumor metastasis of adenoid cystic carcinoma is associated with multiple genes whose function include angiogenesis, protein synthesis, signal transduction, modulation of cell cycle, molecular chaperones, and immune co-stimulating molecule. Moreover, the results of the phenotypic function-related expression mapping analysis at the mRNA and protein level revealed obvious complementarities, providing important clues for further study of the molecular mechanism of metastasis, metastasis control and possible targets for cancer gene therapy.

Serum Overexpression of MicroRNA-10b in Patients with Bone Metastatic Primary Breast Cancer

“…35 The current findings, together with previous studies, suggest a role for miR-10b in cancer and metastatic behaviour. Cancer metastasis is associated with a number of changes in proteins and gene expression, 36 further corroborating the current miR-10b findings.…”

Fluctuation-dissipation ratios in the dynamics of self-assembly

“…Turning to biological systems, it would be possible to measure the degree of kinetic frustration in the folding of biomolecules, either computationally in more detailed capsid models, or in systems such as the trpzip peptide [26], or experimentally, in RNA folding, by a generalization of the experiment of [27]. In this latter case, the conjugate variables of force and displacement are already measurable, although obtaining good statistics for the correlations and responses as a function of both t and t w might be challenging.…”

Observation of multiple folding pathways of β-hairpin trpzip2 from independent continuous folding trajectories

“…Due to the requirement of cooperation of side chains of residues, it is difficult to simulate the complete folding of -hairpin at all-atom level with standard molecular dynamics (MD) simulation method [11,16]. Therefore, most simulation works above were carried out by supplementary methods [17][18][19][20][21][22][23][24][25], such as replica-exchange method, transition-path sampling and reduced model. However, these methods, especially those for all-atom models, usually use abnormal conditions.…”