Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Ding, Ling; Li, Xi; Liu, Peng; Li, Shiqian; Lv, Jiliang

doi:10.1007/s12011-009-8583-7

Cited by 10 publications

(7 citation statements)

References 16 publications

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“…Under conditions of high Cu concentration, intestinal Cu-resistant E. coli were selected, and thus, the resistance of E. coli to Cu increased, resulting in an improvement in the adaptability to high levels of Cu and an increase in the abundance of E. coli. However, some previous studies have indicated that Cu inhibits the growth of E. coli in in vitro culture experiments (Ding et al, 2010;Reyes-Jara et al, 2016). This difference may be explained by the fact that the E. coli strain used for in vitro culturing did not possess a Cu resistance system, while the strains of E. coli in the intestinal tract varied, with some being resistant to Cu.…”

Section: Discussionmentioning

confidence: 97%

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

et al. 2019

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Copper is an essential microelement for animals, and not only it has been used as a feed additive at pharmacological doses in swine production to improve growth performance, but it also has an effect on intestinal microbes by enhancing host bacterial resistance. However, there are few reports on the effects of pharmacological doses of copper on intestinal microorganisms and the antimicrobial resistance profiles of pathogenic bacteria, such as Escherichia coli, in pigs. Therefore, this study aimed to investigate the effects of pharmacological doses of copper on the microbial communities in the hindgut and the antimicrobial resistance profiles of E. coli in weaned piglets. Twenty-four healthy weaned piglets aged 21 ± 1 days and with an average weight of 7.27 ± 0.46 kg were randomly divided into four groups. The control group was fed a basal diet, while the treatment groups were fed a basal diet supplemented with 20, 100, or 200 mg copper/kg feed, in the form of CuSO 4. Anal swabs were collected at 0, 21, and 42 days of the trial, and E. coli was isolated. Meanwhile, the contents of the ileum and cecum from the control and 200 mg copper/kg feed groups were collected at 21 and 42 days for microbial community analysis and E. coli isolation. All isolated E. coli strains were used for antimicrobial resistance profile analysis. A pharmacological dose of copper did not significantly change the diversity, but significantly affected the composition, of microbial communities in the ileum and cecum. Moreover, it affected the microbial metabolic functions of energy metabolism, protein metabolism, and amino acid biosynthesis. Specifically, copper treatment increased the richness of E. coli in the hindgut and the rates of E. coli resistance to chloramphenicol and ciprofloxacin. Moreover, the rate of E. coli resistance to multiple drugs increased in the ileum of pigs

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Section: Discussionmentioning

confidence: 97%

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

et al. 2019

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“…Our present study showed that in three +Se groups, the contents of GSH and the activities of GSH-Px, SOD, GR, and CAT were all increased when compared with AFB 1 group, and the MDA content was decreased. It may be associated with increased antioxidative function resulting from an increase in activity of GSH-Px whose center is Se [46]. As previous study revealed, Se can inhibit lipid peroxidation [47].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Sodium Selenite against Aflatoxin B1-Induced Oxidative Stress and Apoptosis in Broiler Spleen

Wang

Shu

Peng

et al. 2013

IJERPH

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The aim of this study was to investigate the possible protective role of sodium selenite on aflatoxin B1-induced oxidative stress and apoptosis in spleen of broilers. Two hundred one-day-old male broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.3 mg/kg AFB1 + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB1 + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB1 + 0.6 mg/kg Se (+Se group III), respectively. According to biochemical assays, AFB1 significantly decreased the activities of glutathione peroxidase, total superoxide dismutase, glutathione reductase, catalase and the level of glutathione hormone, while it increased the level of malondialdehyde. Moreover, AFB1 increased the percentage of apoptosis cells by flow cytometry and the occurrence of apoptotic cells by TUNEL assay. Simultaneous supplementation with sodium selenite restored these parameters to be close to those in control group. In conclusion, sodium selenite exhibited protective effects on AFB1-induced splenic toxicity in broilers by inhibiting oxidative stress and excessive apoptosis.

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“…In addition, cell numbers drastically decreased when treated with 50 mM or higher Na 2 SeO 3 [ 28 , 29 ]. This phenomenon could be attributed to the interactions between Se and enzymes [ 30 , 31 ], and Se probably exerts inhibiting effect on the activity of enzymes with the catalysis oxidation reactions of S–H groups to S–S or S–Se–S bonds [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating PlantCardamine hupingshanesis

Tong

Yuan

Luo³

et al. 2014

The Scientific World Journal

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A novel selenium- (Se-) hyperaccumulating plant, Cardamine hupingshanesis, accumulating Se as a form of SeCys2, was discovered in Enshi, Hubei, China, which could not be explained by present selenocysteine methyltransferase (SMT) theory. However, it is interesting to investigate if rhizosphere bacteria play some roles during SeCys2 accumulation. Here, one Se-tolerant rhizosphere strain, Microbacterium oxydans, was isolated from C. hupingshanesis. Phylogenetic analysis and 16S rRNA gene sequences determined the strain as a kind of Gram positive bacillus and belonged to the family Brevibacterium frigoritolerans. Furthermore, Se tolerance test indicated the strain could grow in extreme high Se level of 15.0 mg Se L−1. When exposed to 1.5 mg Se L−1, SeCys2 was the predominant Se species in the bacteria, consistent with the Se species in C. hupingshanesis. This coincidence might reveal that this strain played some positive effect in SeCys2 accumulation of C. hupingshanesis. Moreover, when exposed to 1.5 mg Se L−1 or 15.0 mg Se L−1, As absorption diminished in the logarithmic phase. In contrast, As absorption increased when exposed to 7.5 mg Se L−1, indicating As metabolism processes could be affected by Se on this strain. The present study provided a sight on the role of rhizosphere bacteria during Se accumulation for Se-hyperaccumulating plant.

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Study of the Action of Se and Cu on the Growth Metabolism of Escherichia coli by Microcalorimetry

Cited by 10 publications

References 16 publications

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

Effect of Dietary Copper on Intestinal Microbiota and Antimicrobial Resistance Profiles of Escherichia coli in Weaned Piglets

Protective Effects of Sodium Selenite against Aflatoxin B1-Induced Oxidative Stress and Apoptosis in Broiler Spleen

Characterization of a Selenium-Tolerant Rhizosphere Strain from a Novel Se-Hyperaccumulating PlantCardamine hupingshanesis

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