Study of synergistic role of allogenic skin-derived precursor differentiated Schwann cells and heregulin-1β in nerve regeneration with an acellular nerve allograft

Wang, Houlei; Wu, Jingsong; Zhang, Xinchao; Ding, Lei; Zeng, Qingmin

doi:10.1016/j.neuint.2016.04.003

Cited by 19 publications

(22 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4711-50; Biovision, inc., milpitas, ca, uSa) and 1% n 2 supplement (Gibco; thermo fisher Scientific, inc., Waltham, ma, uSa) for 1 week. thereafter, cells appearing to have bipolar Sc morphology under phase-contrast microscopy were isolated with cloning cylinders and expanded in the same medium until >95% purity was achieved (6), and then identified by p75NGFR and S100-β staining, as previously described (12).…”

Section: Methodsmentioning

confidence: 99%

“…ANA procedure. a total of 20 adult male Sd rats (weight, 200-250 g; Shanghai Slac laboratory animal co., ltd., Shanghai, china) were anesthetized with chloral hydrate (350 mg/kg) and subjected to sciatic nerve transection in order to mimic peripheral nerve injury, as previously described (12). rats were maintained under SPf conditions including a 12 h light/12 h dark cycle, temperature of 20-22˚C, and sterilized water and food.…”

Section: Methodsmentioning

confidence: 99%

“…rats were maintained under SPf conditions including a 12 h light/12 h dark cycle, temperature of 20-22˚C, and sterilized water and food. The rats were then treated with anas, as described in a previous study (12). all surgical procedures were performed bilaterally.…”

Section: Methodsmentioning

confidence: 99%

“…Recipient rats were divided into the ana+SKP-Sc-treated group (control group, n=8) and the ana+SKP-Sc+heregulin-1β-treated group (experimental group, n=8), and underwent exposure of the right sciatic nerve. the recipient's nerve was transected at 5 mm proximal to the sciatic trifurcation and 15 mm of nerve was removed under a surgical microscope, as described in our previous study (12). for the two groups, 3 ml of 2x10 6 cells/ml SKP-Scs were injected into the ana at the proximal end and at the distal end 3 mm from the anastomosis, respectively, using a micro-injector under an SXP-10 microscope at x10 magnification (Shanghai Medical equipment Works co., ltd., Shanghai, china).…”

Section: Methodsmentioning

confidence: 99%

“…With the exception of the application of SKP-Scs alone, only concatenated application of the acellular nerve allograft (ana) injection with SKP-Sc has been presented as a better modality for nerve repair compared with intrinsic regeneration (9,10). in addition, in our previous studies, it was found that injection of heregulin-1β, a type of pleiotropic growth factor or neuregulin, further promotes the outcome of reinnervation by the ana with SKP-Sc therapy via regulation of neuronal and glial development (11,12). However, even though the treatment of peripheral nerve injuries with heregulin-1β achieves a notable outcome, the underlying mechanism through which heregulin-1β facilitates ana with SKP-Sc therapy remains unclear.…”

Section: Introductionmentioning

confidence: 95%

See 4 more Smart Citations

Microarray analysis of the expression profile of lncRNAs reveals the key role of lncRNA BC088327 as an agonist to heregulin‑1β‑induced cell proliferation in peripheral nerve injury

Wang

Zhang

et al. 2018

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Heregulin‑1β is capable of promoting the nerve regeneration of acellular nerve allografts with skin‑derived precursor differentiated Schwann cell (SC) therapy in peripheral nerve injury. Long non‑coding RNAs (lncRNAs) serve important roles in the regulation of gene transcription and trans-lation in multiple biological processes, but its association with the repair of peripheral nerve injury is unexplored. Therefore, in the present study, the aim was to identify novel indicators for peripheral nerve injury, and to detect whether there is an association between lncRNA expression and the treatment effect of heregulin‑1β on this disorder. The expression status of lncRNAs and mRNAs in a well‑built rat model with sciatic nerve injury was investigated using microarray assays. Based on the results of the microarray assays and quantitative polymerase chain reaction validation, it was inferred that lncRNA BC088327 was upregulated to the largest extent among all the lncRNAs. According to these findings, the role of BC088327 in peripheral nerve injury was further assessed by detecting the cell viability, cell cycle and apoptosis in a hypoxic SC cell model after suppressing the expression of BC088327 using specific small interfering RNA. Based on the results of the lncRNA microarray assay, 805 lncRNAs were significantly differentially expressed, among which, 323 lncRNAs were upregulated and 482 lncRNAs were downregulated. Based on the results of the mRNA microarray assay, 1,293 lncRNAs were significantly differentially expressed, including 603 upregulated and 690 downregulated lncRNAs. Moreover, knockdown of lncRNA BC088327 suppressed cell viability and induced cell apoptosis and S-phase cell cycle arrest in the SCs. In conclusion, expression profile changes of lncRNAs in peripheral nerve injuries were closely associated with treatment with heregulin‑1β. lncRNA BC088327 may play a synergistic role with heregulin‑1β in repairing peripheral injury, which has the potential be a biomarker for the detection of peripheral injury and a medical target for the development of therapeutic modalities.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations