Study of rubella candidate vaccine based on a structurally modified plant virus

Trifonova, E. A.; Zenin, Vladimir A.; Nikitin, Nikolai A.; Yurkova, Maria S.; Ryabchevskaya, Ekaterina M.; Putlyaev, E. V.; Donchenko, E. K.; Кондакова, О. А.; Федоров, А. Н.; Atabekov, J.G.; Карпова, О. В.

doi:10.1016/j.antiviral.2017.05.006

Cited by 26 publications

(49 citation statements)

References 43 publications

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“…The next candidate to improve PUUV vaccine was a promising plant virus-based adjuvant, SP, derived by thermal transition of Tobacco mosaic virus. In previous studies it was demonstrated that SPs could potentiate immune response to proteins and virions (Karpova et al, 2012;Trifonova et al, 2014;Trifonova et al, 2017). In our experiments, an augmenting immune response was observed at 150 mg/ml SPs at least.…”

supporting

confidence: 73%

“…The unique feature of SPs is their ability to non-specifically adsorb foreign proteins and even non-enveloped virions on the surface (Karpova et al, 2012;Trifonova et al, 2014). Furthermore, SPs can enhance the immune response to the target antigen and act as an adjuvant (Trifonova et al, 2017). In addition, the absence of toxic effects of SPs for laboratory animals was shown (Nikitin et al, 2018a;Nikitin et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

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Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine

Курашова

Ишмухаметов

Dzagurova

et al. 2020

Front. Cell. Infect. Microbiol.

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Various adjuvant effects on the immunogenicity of the candidate inactivated Puumala virus vaccine were detected in BALB/c mice. Adjuvants under study were: aluminum hydroxide, spherical particles of Tobacco mosaic virus coat protein, B subunit of heat-labile enterotoxin of Escherichia coli, and low endotoxic lipopolysaccharide of Shigella sonnei. Aluminum hydroxide (1 mg/ml) did not affect neutralizing antibodies' induction and vaccine stability during storage compared to immunization with the vaccine without adjuvant. B subunit of heat-labile enterotoxin (0.2 µg/ml), low endotoxic lipopolysaccharide (50 µg/ml), and plant virus-based spherical particles (300 µg/ml) significantly enhance the humoral immune response of vaccine (p < 0.0001). Pronounced stimulation of IL-12 and IFN-ɣ was observed when mice were immunized with vaccines both with adjuvants (except of aluminum hydroxide) and without adjuvants. It has been shown that low endotoxic lipopolysaccharide contributes not only to enhance the immune response but also to stabilize vaccine immunogenicity during at least 1 year storage.

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supporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine

Курашова

Ишмухаметов

Dzagurova

et al. 2020

Front. Cell. Infect. Microbiol.

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“…Samsun leaves was described previously [3] . SP samples were prepared from TMV according to [4] . The morphology and size distribution of the obtained TMV spherical particles were subsequently analyzed by transmission electron microscopy and nanoparticle tracking analysis according to [5] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Spherical particles derived from TMV virions enhance the protective properties of the rabies vaccine

et al. 2018

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In this study the ability of spherical particles (SPs) obtained from the tobacco mosaic virus (TMV) virions to enhance the immunogenic potential of the vaccine was evaluated. TMV SPs were shown to increase the protective properties of the widely used effective Russian adjuvant-free rabies vaccine, composed of killed rabies virions. The results of the NIH potency test showed enhancement of protectivity, that is comparable with the effect of the incomplete Freund׳s adjuvant on the same vaccine.

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“…It is important to note that the production of veterinary vaccines based on structurally modified plant viruses (SPs) allows the creation of domestic marker veterinary vaccines, i.e. spherical particles, on which a certain part of antibodies is produced during vaccination with the SP pathogen complex [26], can act as a marker which will further distinguish vaccinated birds from carriers of the field virus.…”

Section: Fig 1 Electron Microphotographs Of Spherical Particles Obtmentioning

confidence: 99%

Development of Avian Influenza Vaccine on the Basis of Structurally Modified Plant Virus

Кондакова¹,

Evtushenko²,

Arkhipenko³

et al. 2017

S-h. biol.

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Avian influenza is an infectious viral disease that affects various species of birds, including poultry (chicken, turkeys, ducks and geese). Vaccination is a key strategy in the prevention of epizootics and epidemics of influenza. Now, the actual problem is the development of rapid, safe and effective methods for the production of avian flu vaccines. The using of recombinant flu antigen is a promising approach for creating universal, safe and effective poultry flu vaccines. To increasing of low immunogenicity of recombinant antigens plant viruses and virus-like particles can be applied. We have previously shown that spherical particles (SPs) with unique adsorption property and immunostimulating activity. Here we design the candidate vaccine against the avian flu virus (H5N1). The complexes containing the antigenic determinants of the avian flu virus-hemagglutinin (HA) and the extracellular domain of the M2 matrix protein (M2e) presented on the surface of SPs were developed. By indirect immunofluorescence microscopy the specific antigenic activity of recombinant proteins adsorbed on the SPs surface were demonstrated. The SPs-HA-M2e (SPs-HA62/284-М2е) complexes were highly immunogenic. The candidate vaccine induced a strong humoral immune response to both antigenic determinants of avian flu A virus. HA and M2e adsorption on the SPs allowed a 10-fold increase in the production of blood antibodies to antigens in immunized animals. The advantage of this approach for the vaccine development is high efficiency based on the stability and adjuvant activity of SPs, safety and low cost of using plant viruses. Obtaining veterinary vaccines based on structurally modified plant viruses allows the creation of marker veterinary vaccines. Development and creation of marker vaccines is extremely important for the recovery of the poultry population in agro-industrial complexes. The proposed vaccine can be considered as a candidate recombinant vaccine against avian influenza virus.

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Study of rubella candidate vaccine based on a structurally modified plant virus

Cited by 26 publications

References 43 publications

Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine

Various Adjuvants Effect on Immunogenicity of Puumala Virus Vaccine

Spherical particles derived from TMV virions enhance the protective properties of the rabies vaccine

Development of Avian Influenza Vaccine on the Basis of Structurally Modified Plant Virus

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