Study of Photosensitizer for Antibacterial Photodynamic Therapy Based on Cyclodextrin Formulation of 133-N-(N-Methylnicotinyl)bacteriopurpurinimide Methyl Ester

Меерович, Г. А.; Ахлюстина, Е В; Тиганова, И. Г.; Панов, А. В.; Тюкова, В. С.; Толордава, Э. Р.; Алексеева, Н. В.; Linkov, Kirill G.; Романова, Ю М; Grin, M. A.; Mironov, A. F.; Лощенов, В. Б.; Каприн, А. Д.; Филоненко, Е. В.

doi:10.24931/2413-9432-2017-6-3-16-32

Cited by 4 publications

(2 citation statements)

References 11 publications

(17 reference statements)

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“…The main ROS include: superoxide radical Ȯ− 2 , hydrogen peroxide H 2 O 2 , hydroxyl O Ḣ and peroxide H Ȯ2 radicals, singlet oxygen 1 O 2 , peroxide ion HO − 2 , etc [1][2][3]. Antimicrobial photodynamic therapy (aPDT) is one of the methods for treating localized inflammatory processes, tumors, and trophic ulcers, where ROS photogeneration underlies the oxidative destruction of pathological cells and tissues upon excitation of a photosensitizer (PS) introduced into the focus of inflammation [4][5][6][7]. An important advantage of aPDT is the multivariance of the nature of oxidative destruction of microbial target cells, which prevents the development of resistance to subsequent photodynamic action [8,9].…”

Section: Introductionmentioning

confidence: 99%

Polymer photosensitizing systems containing porphyrins, dinitrosyl iron complexes, and sodium alginate in the generation of singlet oxygen

Sadykova¹,

Aksenova²,

Глаголев³

et al. 2023

Laser Phys.

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The activity of photosensitizing systems based on water-soluble photoditazine (PDZ) and hydrophobic fluorinated tetraphenylporphyrin (TPPF20) solubilized polyvinylpyrrolidone (PVP) (in the case PDZ) and pluronic F127 (in the case TPPF20) was studied in a model reaction of tryptophan (Trp) photooxidation. We also studied the effect of dinitrosyl iron complex (DNIC) and sodium alginate (SA) polysaccharide on the photosensitizing activity of both PDZ in the presence of PVP and TPPF20 solubilized with pluronic F127 in a model reaction of Trp photooxidation. It was shown that TPPF20 solubilized with pluronic F127 exhibits high photocatalytic activity in the presence of both DNIC and SA. It was also shown that PVP is able to prevent the damaging effect exerted by active NO radicals produced during the photodecomposition of DNIC on PDZ molecules. Moreover, it was demonstrated that SA does not influence the photocatalytic activity of PDZ in the presence and absence of PVP. Using the dynamic light scattering method, it was found that SA macromolecules in the presence of PVP are capable of self-association, which, due to the ‘excluded volume’ effect, can cause a slight increase in the rate of photocatalytic reaction. The effectiveness of using PDZ in combination with PVP in the presence of SA and DNIC as a photosensitizer in antimicrobial photodynamic therapy was studied via in vivo experiments on a model of a full-layer planar wound in laboratory rats. Morphological studies have shown that under photodynamic action on wounds, the use of these systems (compared to photoexcitation of an aqueous solution of the initial PDZ) stimulates the transition of the inflammatory phase to the reparative (proliferative) phase. In addition, the process of cleansing wounds from microflora and devitalized tissues is accelerated, the disturbed microcirculation is restored, the proliferation of fibroblasts and blood vessels, and collagen synthesis are accelerated. It is suggested that the PDZ-PVP-SA-DNIC systems can be used in PDT procedures for the treatment of inflammatory diseases of the skin and soft tissues.

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Section: Introductionmentioning

confidence: 99%

Polymer photosensitizing systems containing porphyrins, dinitrosyl iron complexes, and sodium alginate in the generation of singlet oxygen

Sadykova¹,

Aksenova²,

Глаголев³

et al. 2023

Laser Phys.

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“…В настоящее время наиболее перспективным методом для исследования фармакокинетики препаратов, обладающих выраженными флуоресцентными свойствами, является спектрально-флуоресцентный [1][2][3][4]. Он широко используется для оценки уровня и селективности накопления фотосенсибилизаторов (ФС), предназначенных для фотодинамической терапии и флуоресцентной диагностики, в биологических тканях.…”

Section: Introductionunclassified

Optical spectroanalyzer with extended dynamic range for pharmacokinetic investigations of photosensitizers in biotissue

Меерович

Akhlyustina

Savelieva

et al. 2019

Biomedical photonics

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Currently, the most promising method for the study of pharmacokinetics of drugs with fluorescent properties is the spectral-fluorescent method. In this article, we propose an algorithm for expanding the dynamic range of the spectrum analyzer by automatically monitoring the maximum spectral density in the recorded fluorescence spectrum and automatically controlled changes in the accumulation time depending on this value, followed by compensation of the output signal with regard to this change, as well as hardware circuit solutions that allow this algorithm.Testing of LESA-01-"Biospeс" spectrum analyzer, upgraded using the proposed approach, was carried out on photosensitizer dispersions based on tetra-3-phenylthiophthalocyanine hydroxyaluminium of various concentrations (from 0.01 mg/l to 50 mg/l), approximately corresponding to the concentrations realized in the process of studying pharmacokinetics in calibration samples and tissues of experimental animals.The proposed solutions that implement the algorithm for recording fluorescence spectra with automatic change of accumulation time depending on the signal level, ensured a significant expansion of the dynamic range of the spectrum analyzer (up to 3.5 orders of magnitude) and improved accuracy in pharmacokinetic studies.

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Attenuation correction technique for fluorescence analysis of biological tissues with significantly different optical properties

Savelieva

Kuryanova

Akhlyustina

et al. 2020

Front. Optoelectron.

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Study of Photosensitizer for Antibacterial Photodynamic Therapy Based on Cyclodextrin Formulation of 133-N-(N-Methylnicotinyl)bacteriopurpurinimide Methyl Ester

Cited by 4 publications

References 11 publications

Polymer photosensitizing systems containing porphyrins, dinitrosyl iron complexes, and sodium alginate in the generation of singlet oxygen

Polymer photosensitizing systems containing porphyrins, dinitrosyl iron complexes, and sodium alginate in the generation of singlet oxygen

Optical spectroanalyzer with extended dynamic range for pharmacokinetic investigations of photosensitizers in biotissue

Attenuation correction technique for fluorescence analysis of biological tissues with significantly different optical properties

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