Study of hemocompatibility and endothelial cell interaction of tecoflex-based electrospun vascular grafts

Chernonosova, Vera S.; Гостев, А. А.; Chesаlоv, Yuriy А.; Карпенко, А. А.; Караськов, А. М.; Laktionov, Pavel P.

doi:10.1080/00914037.2018.1525721

Cited by 15 publications

(32 citation statements)

References 61 publications

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“…Scaffolds for blood tests were produced in the same conditions using a drum collector [ 36 ] and different compositions of electrospinning solution as described [ 35 ]. Disks of 10 mm were excised from matrices by die cutting and used as an individual probe.…”

Section: Methodsmentioning

confidence: 99%

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Vascular Stents Coated with Electrospun Drug-Eluting Material: Functioning in Rabbit Iliac Artery

et al. 2020

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A stenting procedure aimed at blood flow restoration in stenosed arteries significantly improves the efficiency of vascular surgery. However, the current challenge is to prevent neointimal growth, which reduces the vessel lumen, in the stented segments in the long run. We tested in vivo drug-eluting coating applied by electrospinning to metal vascular stents to inhibit the overgrowth of neointimal cells via both the drug release and mechanical support of the vascular wall. The blend of polycaprolactone with human serum albumin and paclitaxel was used for stent coating by electrospinning. The drug-eluting stents (DESs) were placed using a balloon catheter to the rabbit common iliac artery for 1, 3, and 6 months. The blood flow rate was ultrasonically determined in vivo. After explantation, the stented arterial segment was visually and histologically examined. Any undesirable biological responses (rejection or hemodynamically significant stenosis) were unobservable in the experimental groups. DESs were less traumatic and induced weaker neointimal growth; over six months, the blood flow increased by 37% versus bare-metal stents, where it increased by at least double the rate. Thus, electrospun-coated DESs demonstrate considerable advantages over the bare-metal variants.

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Section: Methodsmentioning

confidence: 99%

“…The test was executed as recommended by ISO 10993-4 and as previously described [ 36 ]. Briefly, anticoagulated blood was diluted twice with PBS and incubated with 10 mm discs for 1 h. After incubation, cells were pelleted by centrifugation (1000 rpm, 10 min), and optical absorbance of the supernatant was measured at 545 nm.…”

Section: Methodsmentioning

confidence: 99%

Vascular Stents Coated with Electrospun Drug-Eluting Material: Functioning in Rabbit Iliac Artery

et al. 2020

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“…The materials with an implicit fiber orientation were obtained under the described ES conditions [20]. The exposure of GL on the surface of the 3D matrices produced by the ES of polyurethanes ensures that human primary endotheliocytes properly attach to these matrices and proliferate, whereas BV reduces blood cell adhesion [21]. Since poor VG endothelization is one of the causes leading to the formation of neointima, clotting, and stenosing [27,28], we used the VGs produced of Tec-GL with an inner layer of Tec-GL-BV [28] for implantation in animals.…”

Section: Resultsmentioning

confidence: 99%

“…The ES solutions contained 3% Tec-80A (wt/vol) with 15% GL and 3.5% Pel-80A with 10% GL or the same solutions with 1.5% BV [21]. GL and BV concentrations in matrices are shown as the percentage of polyurethane (wt/wt).…”

Section: Electrospinning Of Vascular Graftsmentioning

confidence: 99%

“…When an important fragment of tissue or organ, for example, a region of a blood vessel, is remodeled, the strength of the used construct and its long-term functional stability are the decisive characteristics of such a VG, since its untimely destruction may have a lethal outcome. In earlier papers, we have demonstrated that the 3D matrices produced from protein-enriched polyurethanes possess good mechanical properties, and structural stability in vitro, bio-and hemocompatibilities, and are, thus, valuable materials for the production of small-diameter vascular grafts [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics

et al. 2020

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The biostability of the polyurethanes Tecoflex EG-80A and Pellethane 2363-80A, used as basic polymers of the vascular grafts (VGs) produced by electrospinning, as well as the tensile strength of Tecoflex VGs, are studied. Solutions of Tecoflex or Pellethane with gelatin and bivalirudin in 1,1,1,3,3,3-hexafluoroisopropanol are used for VG production. After 1, 12, and 24 weeks of VG implantation in the infrarenal position of the abdominal aorta of Wistar rats, VGs are explanted, fixed in formalin, freed from outer tissues, dialyzed, and dried. The polyurethanes are extracted from VGs by dispersion/extraction in tetrahydrofuran (THF) and freed from the excess of THF-insoluble biopolymers. The stability of polyurethanes is assessed by IR spectroscopy and gel permeation chromatography. Pellethane has emerged to be stable at all experimental points. Tecoflex loses approximately 10% of its molecular weight (both Mn and Mw) after 3 months and restored its initial value within 6 months of its functioning as a graft. Mechanical testing demonstrates a 30% reduction in the tensile strength after 3 months in VG and a 10% increase after 6 months. The stability and mechanical properties of polyurethane-based VGs demonstrate their utility for the reconstitution of damaged arteries.

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Potential of Biodegradable Synthetic Polymers for Use in Small-diameter Vascular Engineering

Osipova

Laktionov²,

Карпенко³

2022

Macromol. Res.

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Study of hemocompatibility and endothelial cell interaction of tecoflex-based electrospun vascular grafts

Cited by 15 publications

References 61 publications

Vascular Stents Coated with Electrospun Drug-Eluting Material: Functioning in Rabbit Iliac Artery

Vascular Stents Coated with Electrospun Drug-Eluting Material: Functioning in Rabbit Iliac Artery

In Vivo Stability of Polyurethane-Based Electrospun Vascular Grafts in Terms of Chemistry and Mechanics

Potential of Biodegradable Synthetic Polymers for Use in Small-diameter Vascular Engineering

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