Ursodeoxycholic acid (UDCA) is a tertiary bile acid synthesized by the liver and has various functions such as promoting bile secretion, antibacterial effect, inflammatory treatment, cholesterol reduction, and degradation. UDCA seems to be a material that can be applied in many aspects, such as adding it to implant coating materials or wound coatings. However, until now, only experiments and studies have been conducted to administer UDCA orally as a drug. In this paper, research was conducted to serve as a foothold for the application of UDCA to in vitro research. In this study, polymer fibers were prepared using electrospinning technology by mixing UDCA with Polycaprolactone(PCL), a biocompatible polymer. Through various analyses, it was confirmed that UDCA was contained in the electrospun membrane. In addition, it was confirmed that UDCA functions properly through the appearance of antimicrobial properties in antimicrobial experiments. The PCL/UDCA electrospun fiber prepared in this study and the analysis results using it show that UDCA can be applied not only to oral drugs but also to various fields such as wound coverings and stent coating membranes.