Study of glycerol diffusion in skin and myocardium ex vivo under the conditions of developing alloxan-induced diabetes

Abstract

Diabetes mellitus is one of widespread diseases, the development of which impairs the functioning of organism vital organs. The study of the processes that occur in tissues under diabetic conditions facilitates the development of diabetes diagnostics and treatment methods, as well as the prognosis of its complications. The paper reports a comparative study of glycerol diffusion in skin and myocardium of rats ex vivo in the normal condition and the conditions of alloxan-induced diabetes. The glycerol diffusion coefficients in tissues were determined basing on the analysis of kinetics of collimated light transmittance through tissue samples immersed in 70%-glycerol solution. As a result, it was shown that the glycerol diffusion rate in the tissues of rats with alloxaninduced diabetes is essentially decreased as compared to the control group.

Simple multimodal optical technique for evaluation of free/bound water and dispersion of human liver tissue

“…15 The determination of the diffusion coefficients of OCAs and water that are characteristic to these mechanisms is highly important. Some OCAs that have been studied are glucose, 12,16 glycerol, 11,17 dimethyl sulfoxide, mannitol, propylene glycol, and x-ray contrast, such as Trazograph ™ and Omnipaque ™ . 14,18 Such studies have demonstrated a significant decrease in tissue scattering properties and an increase in tissue depth and image contrast when using imaging techniques.…”

Glycerol dehydration of native and diabetic animal tissues studied by THz-TDS and NMR methods

“…In our experiments the thickness of the treated mouse skin samples increase systematically by 5μm (Table 4), which can be explained by the decrease of the transverse dimension [47], since the weight of the treated samples has been decreased as a result of water loss and overall tissue sample shrinkage ( Table 3). The sample thickness increase did not depend on the glycerol concentration because the saturation was observed already at 30% of glycerol concentration during 5 min treatment [48].…”

“…In our experiments some increase of thickness of the treated rat kidney and cornea samples in diabetic and non-diabetic cases have been observed (Table 6), that can be explained by the tissue sample transverse shrinkage [26,47], since the weight of the treated samples has been decreased as a result of water loss. Figure 7 presents kinetics of the transmitted signal of the rat diabetic and non-diabetic kidney and cornea tissues treated with glycerol (70%) at the maximum of THz pulse.…”

Measurement of tissue optical properties in the context of tissue optical clearing

“…To prove the concept, kinetics of tissue permeability for selected OCAs can be studied by providing measurements of temporal collimated transmittance for tissue samples from different organs in the process of their OC. 163,177 Such comparative investigations were performed for ex vivo skin and myocardium samples of Winstar white outbred male rats using 70% glycerol solution as an OCA and the multichannel fiber-optic spectrometer USB4000-Vis-NIR (Ocean Optics, USA) operating in the spectral range of 500 to 900 nm as a measuring system. Animals were divided into the control and diabetic groups.…”