Study of flow behaviors on single-cell manipulation and shear stress reduction in microfluidic chips using computational fluid dynamics simulations

Shen, Feng; Li, Xiu Jun; Li, Paul C. H.

doi:10.1063/1.4866358

Cited by 70 publications

(46 citation statements)

References 40 publications

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“…Particle's dynamic phenomenon in microchannel is often characterized by Re and particle Reynolds number (Re p ) which are defined as 44,47 Re where q f is the density of the liquid, D h is the hydraulic diameter of the microchannel, defined as D h ¼ 2W i H ðW i þHÞ for a microchannel with contraction and expansion structures, 45 U is the fluid velocity in the microchannel, l is the dynamic viscosity of the fluid, U m is the maximum fluid velocity in the microchannel, and a p is the particle diameter.…”

Section: Working Principlementioning

confidence: 99%

Continuous size-based separation of microparticles in a microchannel with symmetric sharp corner structures

Fan

Han

et al. 2014

Biomicrofluidics

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A new microchannel with a series of symmetric sharp corner structures is reported for passive size-dependent particle separation. Micro particles of different sizes can be completely separated based on the combination of the inertial lift force and the centrifugal force induced by the sharp corner structures in the microchannel. At appropriate flow rate and Reynolds number, the centrifugal force effect on large particles, induced by the sharp corner structures, is stronger than that on small particles; hence after passing a series of symmetric sharp corner structures, large particles are focused to the center of the microchannel, while small particles are focused at two particle streams near the two side walls of the microchannel. Particles of different sizes can then be completely separated. Particle separation with this device was demonstrated using 7.32 μm and 15.5 μm micro particles. Experiments show that in comparison with the prior multi-orifice flow fractionation microchannel and multistage-multiorifice flow fractionation microchannel, this device can completely separate two-size particles with narrower particle stream band and larger separation distance between particle streams. In addition, it requires no sheath flow and complex multi-stage separation structures, avoiding the dilution of analyte sample and complex operations. The device has potentials to be used for continuous, complete particle separation in a variety of lab-on-a-chip and biomedical applications.

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Section: Working Principlementioning

confidence: 99%

Continuous size-based separation of microparticles in a microchannel with symmetric sharp corner structures

Fan

Han

et al. 2014

Biomicrofluidics

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“…where Re is the Reynold's number (qVD/l), 43 l is the dynamic viscosity of the fluid, L is the wetted length, V is the flow velocity, and q is the density. By equating frictional force to capillary force based on the above two equations, we can determine the auto-mixing velocity by the following relation:…”

Section: Resultsmentioning

confidence: 99%

3D printed auto-mixing chip enables rapid smartphone diagnosis of anemia

et al. 2016

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Clinical diagnosis requiring central facilities and site visits can be burdensome for patients in resource-limited or rural areas. Therefore, development of a lowcost test that utilizes smartphone data collection and transmission would beneficially enable disease self-management and point-of-care (POC) diagnosis. In this paper, we introduce a low-cost iPOC 3D diagnostic strategy which integrates 3D design and printing of microfluidic POC device with smartphone-based disease diagnosis in one process as a stand-alone system, offering strong adaptability for establishing diagnostic capacity in resource-limited areas and low-income countries. We employ smartphone output (AutoCAD 360 app) and readout (color-scale analytical app written in-house) functionalities for rapid 3D printing of microfluidic auto-mixers and colorimetric detection of blood hemoglobin levels. The automixing of reagents with blood via capillary force has been demonstrated in 1 second without the requirement of external pumps. We employed this iPOC 3D system for point-of-care diagnosis of anemia using a training set of patients (n anemia ¼ 16 and n healthy ¼ 6), which showed consistent measurements of blood hemoglobin levels (a.u.c. ¼ 0.97) and comparable diagnostic sensitivity and specificity, compared with standard clinical hematology analyzer. Capable of 3D fabrication flexibility and smartphone compatibility, this work presents a novel diagnostic strategy for advancing personalized medicine and mobile healthcare. Published by AIP Publishing. [http://dx

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“…2), keeping the second worm from loading. 37,47 An injection channel (420 lm wide) is connected to the side of the immobilization channel and has an open end that allows a micropipette to reach the immobilized worm ( Fig. 1(b)).…”

Section: A Device Design and Fabricationmentioning

confidence: 99%

A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

2016

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The nematode worm Caenorhabditis elegans has been widely used as a model organism in biological studies because of its short and prolific life cycle, relatively simple body structure, significant genetic overlap with human, and facile/inexpensive cultivation. Microinjection, as an established and versatile tool for delivering liquid substances into cellular/organismal objects, plays an important role in C. elegans research. However, the conventional manual procedure of C. elegans microinjection is labor-intensive and time-consuming and thus hinders large-scale C. elegans studies involving microinjection of a large number of C. elegans on a daily basis. In this paper, we report a novel microfluidic device that enables, for the first time, fully automated, high-speed microinjection of C. elegans. The device is automatically regulated by on-chip pneumatic valves and allows rapid loading, immobilization, injection, and downstream sorting of single C. elegans. For demonstration, we performed microinjection experiments on 200 C. elegans worms and demonstrated an average injection speed of 6.6 worm/min (average worm handling time: 9.45 s/ worm) and a success rate of 77.5% (post-sorting success rate: 100%), both much higher than the performance of manual operation (speed: 1 worm/4 min and success rate: 30%). We conducted typical viability tests on the injected C. elegans and confirmed that the automated injection system does not impose significant adverse effect on the physiological condition of the injected C. elegans. We believe that the developed microfluidic device holds great potential to become a useful tool for facilitating high-throughput, large-scale worm biology research. V C 2016 AIP Publishing LLC.

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Study of flow behaviors on single-cell manipulation and shear stress reduction in microfluidic chips using computational fluid dynamics simulations

Cited by 70 publications

References 40 publications

Continuous size-based separation of microparticles in a microchannel with symmetric sharp corner structures

Continuous size-based separation of microparticles in a microchannel with symmetric sharp corner structures

3D printed auto-mixing chip enables rapid smartphone diagnosis of anemia

A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

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