Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/ B. vulgaris  extract composite scaffold

Hosseinzadeh, Simzar; Soleimani, Masoud; Vossoughi, M.; Ranjbarvan, Parviz; Hamedi, Shokoh; Benisi, Soheila Zamanlui; Mahmoudifard, Matin

doi:10.1016/j.msec.2017.02.101

Cited by 19 publications

(10 citation statements)

References 28 publications

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“…Electrospinning is a popular and simple method to produce fibers with diameter ranging from several micrometers down to a few nanometers. The electrospun scaffolds have been widely studied and used for different biomedical applications such as wound dressing . Many investigations have been carried out on utilizing bilayered or multilayered scaffolds that not only allows for combining the capabilities of the constitutive layers such as degradation, drug release, and mechanical characteristics, but also provides a proper substrate for cells to attach and proliferate under a desired combination of conditions .…”

Section: Introductionmentioning

confidence: 99%

Electrospun triple‐layered PLLA/gelatin. PRGF/PLLA scaffold induces fibroblast migration

Piran

Shiri

Zomorrod

et al. 2019

J of Cellular Biochemistry

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The function of fibroblast cells in wounded areas results in reconstruction of the extra cellular matrix and consequently resolution of granulation tissue. It is suggested that the use of platelet-rich plasma can accelerate the healing process in nonhealing or slow-healing wounds. In this study, a simple and novel method has been used to fabricate an electrospun three-layered scaffold containing plasma rich in growth factor with the aim of increasing the proliferation and migration of fibroblast cells in vitro. First, plasma rich in growth factor was derived from platelet rich plasma, and then a three-layered scaffold was fabricated using PLLA nanofibers as the outer layers and plasma rich in growth factor-containing gelatin fibers as the internal layer. The growth morphology of cells seeded on this scaffold was compared to those seeded on one layered PLLA scaffold. The study of the cell growth rate on different substrates and the migration of cells in response to the drug release of multilayered scaffold was investigated by the cell quantification assay and a modified under agarose assay. Scanning electron microscopy and fluorescence images showed that cells seeded on multilayered scaffold were completely oriented 72 hours after seeding compared to those seeded on PLLA scaffold. The cell quantification assay also indicated significant increase in proliferation rate of cells seeded on three-layered scaffold compared to those seeded on PLLA scaffold and finally, monitoring cell migration proved that cells migrate significantly toward the three-layered scaffold up to 48 to 72 hours and afterwards start to show a diminished migration rate toward this scaffold. K E Y W O R D S electrospinning, fibroblast migration, multilayered scaffolds, platelet rich plasma, skin tissue engineering

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Section: Introductionmentioning

confidence: 99%

Electrospun triple‐layered PLLA/gelatin. PRGF/PLLA scaffold induces fibroblast migration

Piran

Shiri

Zomorrod

et al. 2019

J of Cellular Biochemistry

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“…Different scaffolds were introduced, yet chitosan is the most common 26,27 . As a result of several advantages including cell proliferation promotion and removing exudates from the wound site, scaffolds have been considered as a promising substrate for tissue engineering, especially for repairing damaged skin [28][29][30][31][32][33] .…”

Section: Wwwwjpsirmentioning

confidence: 99%

Evaluation of Acellular Dermal Matrix (ADM) as a Scaf-fold for Adipose-Derived Stem Cell Transfer in the Rat Model

Jahanian¹,

Hoseini²,

Atashi³

et al. 2021

WJPS

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“…The obtained nanofiber membrane had a high porosity with a small pore size. The results of the cell proliferation assay, as well as the study of the keratinocyte culture on this scaffold, introduced this scaffold as an excellent structure in the field of skin tissue engineering (Hosseinzadeh et al, 2017; Khan, Xiangyang, Ahmad, & Mo, 2018).…”

Section: The Types Of Plant Compounds Used In Electrospinningmentioning

confidence: 99%

Medicinal plants used in wound dressings made of electrospun nanofibers

Fatehi

Abbasi

2020

J Tissue Eng Regen Med

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Nanofibers are a type of nanostructures, which due to their unique properties can be used in wound dressing, and electrospinning is a good way to produce them. The benefits of wound dressings made of electrospun nanofibers include a large surface area to volume ratio, high absorption of secretions from the wound, and high air permeability, mimicking the morphology of extracellular matrix (ECM) of the damaged tissue and the possibility of the gradual release of the drug agents loaded on nanofibers. Because of the adaptability of plants to the body, low side effects and the prevalence of antibiotic resistance, interest in using plants is increasing. Combining nanofibers with plants is a way to integrate the physical properties of the structure of nanofibers and the chemical and antibacterial properties of the plants. In recent years, many plants in the forms of extracts, essential oils, and pure active ingredients have been used in the electrospininng and production of nanofibercontaining plants; some of the plants may be a good choice for wound dressings made of electrospun nanofibers.

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Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/ B. vulgaris extract composite scaffold

Cited by 19 publications

References 28 publications

Electrospun triple‐layered PLLA/gelatin. PRGF/PLLA scaffold induces fibroblast migration

Electrospun triple‐layered PLLA/gelatin. PRGF/PLLA scaffold induces fibroblast migration

Evaluation of Acellular Dermal Matrix (ADM) as a Scaf-fold for Adipose-Derived Stem Cell Transfer in the Rat Model

Medicinal plants used in wound dressings made of electrospun nanofibers

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