Evaluation of Acellular Dermal Matrix (ADM) as a Scaf-fold for Adipose-Derived Stem Cell Transfer in the Rat Model

Jahanian, Maryam; Hoseini, Sara; Atashi, Amir; Saberi, Mohsen; Hoseini, Seyyed Aboozar; Mozaffari, Kambiz; Fatemi, Mohammad Javad

doi:10.52547/wjps.10.2.67

Cited by 4 publications

(3 citation statements)

References 55 publications

(34 reference statements)

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“…Our results reported more significant signs of repair than those reported in a study on the same biomodel using decellularized fetal bovine skin substitute with autologous skin cell suspension [ 69 ], indicating that the wound closed in approximately 42 days after treatment and according to the histological result, the immature epithelium and presence of inflammatory cells in the wound treated with decellularized skin substitute were observed at 28 days. Likewise, the skin repair potential of construct 1 and hADM described here was higher than similar strategies evaluated in different animal models [ 67 , 70 , 71 ]. Although we did not use a positive control such as Integra™ seeded with keratinocytes in our in vivo assay, previous studies have shown that this alternative, evaluated in a porcine biomodel, fails to achieve epithelial stratification [ 72 ], and also produces hyperkeratinized scarring at week 8 of evaluation [ 73 ].…”

Section: Discussionmentioning

confidence: 69%

Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair

Correa-Araujo,

Prieto-Abello,

Lara-Bertrand

et al. 2023

Stem Cell Res Ther

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Background Skin tissue engineering is a rapidly evolving field of research that effectively combines stem cells and biological scaffolds to replace damaged tissues. Human Wharton’s jelly mesenchymal stromal cells (hWJ-MSCs) are essential to generate tissue constructs, due to their potent immunomodulatory effects and release of paracrine factors for tissue repair. Here, we investigated whether hWJ-MSC grown on human acellular dermal matrix (hADM) scaffolds and exposed to a proinflammatory environment maintain their ability to produce in vitro growth factors involved in skin injury repair and promote in vivo wound healing. Methods We developed a novel method involving physicochemical and enzymatic treatment of cadaveric human skin to obtain hADM scaffold. Subsequently, skin bioengineered constructs were generated by seeding hWJ-MSCs on the hADM scaffold (construct 1) and coating it with human platelet lysate clot (hPL) (construct 2). Either construct 1 or 2 were then incubated with proinflammatory cytokines (IL-1α, IL-1β, IL-6, TNF-α) for 12, 24, 48, 72 and 96 h. Supernatants from treated and untreated constructs and hWJ-MSCs on tissue culture plate (TCP) were collected, and concentration of the following growth factors, bFGF, EGF, HGF, PDGF, VEGF and Angiopoietin-I, was determined by immunoassay. We also asked whether hWJ-MSCs in the construct 1 have potential toward epithelial differentiation after being cultured in an epithelial induction stimulus using an air–liquid system. Immunostaining was used to analyze the synthesis of epithelial markers such as filaggrin, involucrin, plakoglobin and the mesenchymal marker vimentin. Finally, we evaluated the in vivo potential of hADM and construct 1 in a porcine full-thickness excisional wound model. Results We obtained and characterized the hADM and confirmed the viability of hWJ-MSCs on the scaffold. In both constructs without proinflammatory treatment, we reported high bFGF production. In contrast, the levels of other growth factors were similar to the control (hWJ-MSC/TCP) with or without proinflammatory treatment. Except for PDGF in the stimulated group. These results indicated that the hADM scaffold maintained or enhanced the production of these bioactive molecules by hWJ-MSCs. On the other hand, increased expression of filaggrin, involucrin, and plakoglobin and decreased expression of vimentin were observed in constructs cultured in an air–liquid system. In vivo experiments demonstrated the potential of both hADM and hADM/hWJ-MSCs constructs to repair skin wounds with the formation of stratified epithelium, basement membrane and dermal papillae, improving the appearance of the repaired tissue. Conclusions hADM is viable to fabricate a tissue construct with hWJ-MSCs able to promote the in vitro synthesis of growth factors and differentiation of these cells toward epithelial lineage, as well as, promote in a full-thickness skin injury the new tissue formation. These results indicate that hADM 3D architecture and its natural composition improved or maintained the cell function supporting the potential therapeutic use of this matrix or the construct for wound repair and providing an effective tissue engineering strategy for skin repair.

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Section: Discussionmentioning

confidence: 69%

Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair

Correa-Araujo,

Prieto-Abello,

Lara-Bertrand

et al. 2023

Stem Cell Res Ther

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“…(англ. M. Jahanian et al) [32] Крысы Аппликация СКЖТ в составе ацеллюлярного дермального матрикса на полнослойную рану В нашем исследовании использовались внутрикожные инъекции в раневое ложе на глубину до 2 мм (мезотерапия) и подкожные инъекции вокруг и под дно раны. Глубина внутрикожных инъекций выбрана минимальная, чтобы приблизить ММСК к зоне регенеративных процессов.…”

Section: мышиunclassified

“…Опыт нанесения механически обработанной СВФ на раневую поверхность ран в целях стимуляции процесса регенерации ран свидетельствует о целесообразности этого метода только в отношении ограниченных по площади ран с неоднородным рельефом дна [34]. Ферментативно обработанная СВФ оптимальна для лечения обширных ран, однако для фиксации стволовых клеток на поверхности донорской раны необходимо применение дополнительных агентов, таких как фибриновый клей или матрицы-носители, которые сами могут оказывать непосредственное влияние на процесс регенерации [24,28,31,32].…”

Section: мышиunclassified

Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Koloshein,

Ryabkov,

Egorikhina

et al. 2024

Урал. мед. журнал

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Introduction. The optimal method of obtaining and application a stromal-vascular fraction (SVF) of adipose tissue in the treatment of donor wounds in burned patients has not been determined.Objective — to compare the effectiveness of the stromal-vascular fraction of adipose tissue in the treatment of donor wounds, depending on the method of obtaining (mechanical and enzymatic) and the injection (subdermal, intradermal) into the tissue.Material and methods. Wistar rats (n = 30) were divided into 2 investigated groups: in the first group (eSVF) enzymatic processing of adipose tissue (n = 10) was used, in the second group (mSVF) mechanical processing was used (n = 10). Another 10 rats were used as adipose tissue donors’ group. A deep burn was created in all animals, and after 4 days two more donor wounds were created by taking a split thickness skin autograft: the stromal-vascular fraction was injected intradermally into one of the wounds, subdermally into the other. After 14 days, the area of epithelialization, the microcirculation index and the histological microstructure of the skin were evaluated.Results. The proportion of completely epithelized donor wounds in the eSVF-group was 85 %, in the mSVF-group — 55 % (p < 0.05). The index of microcirculation significantly decreased after intradermal injections in the eSVF-group (p < 0.01), and after subdermal injections — in the mSVF-group (p < 0.05). According to histomorphometry, with intradermal injections of eSVF, the thickness of the epidermis and the layer of granulation tissue is less than in the mSVF group (p < 0.01). At the same time, the relative density of collagen fibers in the granulation tissue was significantly higher after the injections of eSVF than after mSVF (p < 0.01). Comparison of injections of the stromal-vascular fraction showed: no significant differences were found when using eSVF; when using mSVF, the subdermal injections are preferable, since it was accompanied by a significantly lower thickness of granulation tissue (p < 0.01).Conclusions. The enzymatically obtained stromal-vascular fraction of adipose tissue has advantages over the mechanically obtained one: the efficiency of eSVF is equally high when used with intradermally and subdermally injections, which are confirmed by the structure and blood circulation in the newly formed skin. When using mSVF, intradermal injections are the least effective, and subdermal injections are accompanied by a moderately pronounced positive dynamic in the microstructure of the skin of donor wounds.

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Modulation of Mesenchymal Stem Cells-Mediated Adaptive Immune Effectors’ Repertoire in the Recovery of Systemic Lupus Erythematosus

Hoseinzadeh

Rezaieyazdi

Afshari

et al. 2022

Stem Cell Rev and Rep

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Evaluation of Acellular Dermal Matrix (ADM) as a Scaf-fold for Adipose-Derived Stem Cell Transfer in the Rat Model

Abstract: BACKGROUNDThis study was designed for the evaluation of Acellular Dermal Matrix (ADM) as a scaffold for adipose-derived stem cell transferring in the rat model. METHODS This experimental study was done in the

Cited by 4 publications

References 55 publications

Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair

Bioengineered skin constructs based on mesenchymal stromal cells and acellular dermal matrix exposed to inflammatory microenvironment releasing growth factors involved in skin repair

Comparison of the Effectiveness of Methods for Obtaining and Application the Stromal-Vascular Fraction of Adipose Tissue in an Experimental Model of a Donor Wound in Burns

Modulation of Mesenchymal Stem Cells-Mediated Adaptive Immune Effectors’ Repertoire in the Recovery of Systemic Lupus Erythematosus

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