Study of DNA–ellipticine interaction by capillary electrophoresis with laser‐induced fluorescence detection

Abstract

Ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence (CE-LIF) detection has been developed for the determination of ellipticine and for the monitoring of ellipticine-DNA interaction. Sodium acetate (50 mM, pH 4.5) was used as a background electrolyte and LIF detection at λ(ex) = 488 nm. The limit of detection for ellipticine was determined to be 5 × 10⁻⁸ M. A total of 20% dimethyl sulfoxide was found optimal as sample solvent. Additionally, intercalation of ellipticine into the double-stranded DNA was investigated. Signal corresponding to ellipticine was decreasing and a new peak appeared and was growing. It can be concluded that CE-LIF is a method applicable to in vitro studies of ellipticine-DNA complexes.

A spectroscopic investigation and molecular docking study on the interaction of hen egg white lysozyme with liposomes of saturated and unsaturated phosphocholines probed by an anticancer drug ellipticine

“…plants. 9 Ellipticine and its derivatives have been found to have anticancer activity since the 1960s. [10][11][12][13] Ellipticine exists in both cationic and neutral forms at physiological pH (pK a 7.4).…”

Partial chemical characterization of the yeast extracts Lachancea thermotolerans CCMA 0763

“…They have been found in bacteria, myxomycetes, fungi, sponges, tunicates and in the related plant families Apocynaceae and Loganiaceae. The vast majority of Carbazoles, comprising more than 330 derivatives, have been shown to be derived from 3-Methylcarbazole as a common precursor (Ryvolova et al, 2012). The vast majority of Carbazoles, comprising more than 330 derivatives, have been shown to be derived from 3-Methylcarbazole as a common precursor.…”

Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

“…Several analytical methods, for instance, spectroscopic techniques , electrophoresis and electrochemical techniques have been carried out for the determination of the interaction between DANN or IDER. Electrochemical techniques offer a wide range of usage in the investigation of the interaction between anticancer drugs and DNA due to some properties such as easy fabrication, fast response time, low cost, and the possibility of instrument miniaturization.…”