Electrochemical Determination of the Effect of Caffeic Acid onto the Interaction between Idarubicin and DNA by Single‐use Disposable Electrodes

Abstract

Now it is well known, antioxidant may affect the interaction of anticancer drugs and DNA. This study aims to investigate the interaction between Idarubicin and DNA and the effect of caffeic acid on this interaction. Disposable, inexpensive, easy handle electrodes were used in this study to investigate the interaction of idarubicin and DNA electrochemically. Idarubicin (IDR) is an anthracyline antitumor antibiotic, which used against one or more types of leukemia. Electrochemical behaviour of IDR was investigated by using cyclic voltammetry. The interaction between anticancer drug, IDR and calf thymus double‐stranded DNA (ctdsDNA) was investigated by using differential pulse voltammetry (DPV) technique in the absence and presence of caffeic acid.

Electrochemical DNA Sensor Based on Poly(Azure A) Obtained from the Buffer Saturated with Chloroform

“…From other sensors utilized in determination of biologically active low molecular compounds, DNA-based assay is considered as one of most promising due to high variety of the species detected, higher stability of DNA oligonucleotides and aptamers against proteins, and simpler detection mode [ 9 , 10 ]. In particular, DNA-based sensors have been described for sensitive determination of antitumor drugs [ 11 , 12 , 13 , 14 ], reactive oxygen species [ 15 ], and antioxidants [ 16 , 17 , 18 ]. Aptamer-based biosensors detect with very high sensitivity mycotoxins, drugs, and cancer biomarkers [ 19 , 20 , 21 , 22 , 23 , 24 , 25 ].…”