Studies With Inagglutinable Erythrocyte Counts. I. A Method for Measurement of Net Gain or Deficit of Red Cells in the Human Subject

Abstract

In 1919 Winifred Ashby (1) reported a method of studying the survival of transfused erythrocytes in the circulation of the recipient by means of differential agglutination. Blood of group 0 was transfused to patients of group A or B and measured specimens of the recipient's blood were mixed with appropriate anti-A or anti-B serum. After the recipient's cells were agglutinated, the inagglutinated cells were counted. The resulting values, minus a blank estimated on the recipient's cells, were considered as representative of the concentration of group 0 blood in the circulation. By this method the survival rate of transfused erythrocytes was studied for many days. In 1928 the method was extended by Landsteiner, Levine, and Janes (2) so that the survival of transfused cells of type N could be followed in the circulation of the type M recipient by counting the cells which were not agglutinated by anti-M serum.

Studies With Inagglutinable Erythrocyte Counts. V. Spontaneous and X-Ray-Induced Hemolysis in Malignancy 12

“…The data in these studies were obtained by application of the method described by DeGowin, Sheets, and Hamil-ton of Ashby's technique of differential agglutination of erythrocytes (12). The Our methods of enumerating erythrocyte and inagglutinable cells have been reported and discussed in detail previously (12,13).…”

Studies With Inagglutinable Erythrocyte Counts. Ii. Analysis of Mechanism of Cooley's Anemia 1

“…of blood were withdrawn from a subject with Cooley's trait (Case 4) who was then transfused with an equivalent amount of freshly collected group 0 blood from a normal donor. An analysis by the method of DeGowin, Sheets and Hamilton (10) The values for obs T, obs R, exp R, and exp R + exp R' are plotted in the upper graph and the observed values for the inagglutinable cell counts (obs D) occur in the lower part on the same scale. The inagglutinable cells disappear from the circulation in 120 days.…”

“…This method has been evaluated by us in another paper (10). Simultaneous counts of the inagglutinable cells and the total erythrocytes were obtained throughout the studies.…”

Studies With Inagglutinable Erythrocyte Counts. Vi. Accelerated Destruction of Normal Adult Erythrocytes in Pernicious Anemia; Contribution of Hemolysis to the Oligocythemia 12

“…After transfusion the donor's cell counts were estimated in the recipient's circulation by a modification of the Ashby differential agglutination technique previously described and evaluated by us (7,8 …”