Studies on the synthesis, characterization, human serum albumin binding and biological activity of single chain surfactant–cobalt(III) complexes

Abstract

The interaction of surfactant-cobalt(III) complexes [Co(bpy)(dien)TA](ClO4)3 · 3H2O (1) and [Co(dien)(phen)TA](ClO4)3 · 4H2O (2), where bpy = 2,2'-bipyridine, dien = diethylenetriamine, phen = 1,10-phenanthroline and TA = tetradecylamine with human serum albumin (HSA) under physiological conditions was analyzed using steady state, synchronous, 3D fluorescence, UV/visabsorption and circular dichroism spectroscopic techniques. The results show that these complexes cause the fluorescence quenching of HSA through a static mechanism. The binding constant (Kb ) and number of binding-sites (n) were obtained at different temperatures. The corresponding thermodynamic parameters (∆G°, ∆H° and ∆S°) and Ea were also obtained. According to Förster's non-radiation energy transfer theory, the binding distance (r) between the complexes and HSA were calculated. The results of synchronous and 3D fluorescence spectroscopy indicate that the binding process has changed considerably the polarity around the fluorophores, along with changes in the conformation of the protein. The antimicrobial and anticancer activities of the complexes were tested and the results show that the complexes have good activities against pathogenic microorganisms and cancer cells.

Bioreduction of silver nanoparticles characterizations and their antimicrobial activity

“…They reported the highest being Ca, Mg and S with 63.5%, 11.9% and 10.72% respectively and the absence of Fe although it is found in very low propositions. As EDS equipment works at low vacuum (1-270 pa) it allows to observe non-conducting samples without the need to cover them with a thin conductive film, and consequently no evidence of noise by the coating material [18,19]. Dynamic light scattering (DLS) is a technique used to determine the size, size distribution profile and poly disparity index of particles in a colloidal suspension.…”

Herbal derived nanomedicines against for some human pathogens

“…The spherical shaped silver nanoparticles having size in range of 16-28 nm were achieved using this extract with antibacterial property observed by Kirby-Bauer method against multi-drug resistant bacteria such as Streptococcus pyogens, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Staphylococcus aureus [18]. A silver nanoparticle of size 22 nm was synthesized using extracts of the tree Cocous nucifera in ethyl acetate and methanol (in ratio of EA: M40:60).…”

“…It showed significant antimicrobial activity against human bacterial pathogens, viz. Salmonella paratyphi, Klebsiella pneumoniae, Bacillus subtilis and Pseudomonas aeruginosa [18,20]. A stable and spherical shaped silver nanoparticle was synthesized using extract of Abutilon indicum.…”

Synthesis of Evolvulus alsinoides derived gold nanoparticles for medical applications

“…The test sample was challenged against certain microbial strains (procured from MTCC and NCIM, India) for antimicrobial sensitivity using the disc diffusion method [13] [17]. The 15 and 30 μL of sample coated disc were placed in agar plates, separately.…”