Studies on the reticulo-endothelial system of the dogfish, Scyliorhinus canicula

Hunt, T. C.; Rowley, Andrew F.

doi:10.1007/bf00218402

Cited by 13 publications

(26 citation statements)

References 27 publications

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“…However, significant numbers were not identified in the flow cytometry bit-map characteristic of these cells (Hamdani et al, 1998;Secombes, 1996). Phagocytes have also been identified in the gills of rainbow trout (Smith, 1982;Goldes et al, 1986), the gills of the dogfish (Hunt & Rowley, 1986) and the river lamprey, Lampetra fluviatilis (Page & Rowley, 1984). However, assays of the respiratory burst of all the isolated gill preparations were negative whereas head kidney cells obtained from the same fish and assayed at the same time were positive.…”

Section: Discussionmentioning

confidence: 80%

Migratory, respiratory burst and mitogenic responses of leucocytes isolated from the gills of rainbow trout (Oncorhynchus mykiss)

Lin

Ellis

Davidson

et al. 1999

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 80%

Migratory, respiratory burst and mitogenic responses of leucocytes isolated from the gills of rainbow trout (Oncorhynchus mykiss)

Lin

Ellis

Davidson

et al. 1999

Fish & Shellfish Immunology

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“…Many authors have reported that vascular injection or the in vitro interaction with foreign particulate material (colloidal thorium dioxide, silica, fine latex beads, ferritin, or saccharated iron oxide) results in association of the particulate material with the cytoplasm of thrombocytes and platelets (Lewis et al, 1976;Awadhiya et al, 1980;Grecchi et al, 1980;Morrow and Pulsford, 1980;1982;van Alten, 1980;Hunt and Rowley, 1986;Muñ oz, 1993). In most cases, doubt has been expressed as to whether this association represents true endocytosis, since the particulate material seems to be inside the canalicular system and so in contact with the extracellular space.…”

Section: Interaction Of Thrombocytes or Platelets Withmentioning

confidence: 96%

Are thrombocytes and platelets true phagocytes?

Meseguer¹,

Esteban²,

Rodrı́guez³

2002

Microscopy Res & Technique

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Thrombocytes and platelets, beyond their primary function in hemostasis, seem to play an active role in inflammation. As regards their phagocytic ability, the results to date are confusing, incomplete, and somewhat contradictory. Whereas the interaction of avian thrombocytes or mammalian platelets with bacteria both in vitro and in vivo has received wide attention, almost no information exists on the topic in "lower" vertebrates. The aim of this work is to review the available information on the phagocytic properties of thrombocytes and platelets. Particular attention is payed to the ontogeny of these cells, the soluble factors involved in the inflammatory process derived from them, and their interaction with particulate material, mainly with bacteria.

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“…Following primary fixation the tissues were washed in isosmotic sodium cacodylate buffer, postfixed in 1% osmium tetroxide, dehydrated in methanol, and embedded in Araldite; thick (0.5-1.5 p.m) and thin sections were then cut. The staining procedure and full details of the fixation schedule are described elsewhere (Hunt and Rowley 1986).…”

Section: Processing For Electron Microscopymentioning

confidence: 99%

“…Fish were injected intravascularly as described in detail previously (Hunt and Rowley 1986). Three fish were used for each of the time periods employed and for each of the materials injected.…”

Section: Injection Proceduresmentioning

confidence: 99%

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Studies on the reticuloendothelial system of the dogfish Scyliorhinus canicula. Endocytic activity of fixed cells in the spleen and liver

Hunt¹,

Rowley²

1987

Can. J. Zool.

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HUNT, T. C., and ROWLEY, A. F. 1986. Studies on the reticuloendothelial system of the dogfish Scyliorhinus canicula. Endocytic activity of fixed cells in the spleen and liver. Can. J. Zool. 65: 1759-1769. The role of the spleen and liver of the lesser spotted dogfish, Scyliorhinus canicula, in the clearance of a range of materials, including colloidal carbon, latex beads, sheep red blood cells, bacteria, and dextran, was examined. The spleen was particularly important in the clearance process. Smaller particulates, such as carbon, were sequestered by the highly endocytic macrophages of the ellipsoids, while larger particles, for example, bacteria and sheep red blood cells, were mainly taken up by the fixed macrophages of the red pulp. An increase in the numbers of the eosinophilic G 1 granulocytes in the red pulp of the spleen was observed following injection of the particulates. In the liver, fixed cells, probably homologous to mammalian Kupffer cells, were involved in clearance and participated in endocytosis of carbon and latex. Additionally, all of the injected particulates were internalized by cells (frequently seen to also contain melanin granules) lying within nonsinusoidal blood vessels in the hepatic parenchyma. Often, but especially in fish injected with bacteria, these phagocytic cells appeared trapped along with free bacteria, within large clumps of circulatory blood cells in the vessels. The presence of the injected materials in the spleen and liver was evident at 30 min and increased up to 1 week. The ultimate localization and differential uptake of the injected materials, and the relative importance of the spleen and liver in the clearance of these materials are discussed.HUNT, T. C., et ROWLEY, A. F. 1986. Studies on the reticuloendothelial system of the dogfish Scyliorhinus canicula. Endocytic activity of fixed cells in the spleen and liver. Can. J. Zool. 65 : 1759-1769. Le r61e de la rate et du foie dans la clearance mdtabolique d'un certain nombre de substances (carbone colloi'dal, billes de latex, Crythrocytes de moutons, bactCries, dextran), a Ct C examink chez le requin Scyliorhinus canicula. La rate joue un r61e particulierement important dans le processus. Les particules plus fines, particules de carbone par exemple, sont sCquestrCes par les macrophages des ellipsoi'des qui assurent une endocytose efficace, alors que les particules plus grosses, bactCries et Crythrocytes de mouton par exemple, sont absorbdes surtout par les macrophages fixes de la pulpe rouge. On remarque une augmentation du nombre de granulocytes Cosinophiles GI dans la pulpe rouge de la rate apres injection des particules. Dans le foie, des cellules fixes, probablement homologues des cellules de Kupffer des mammiferes, jouent un r61e dans la clearance et elles assurent I'endocytose du carbone et du latex. De plus, tous les types de particules injectCes sont Cgalement sCquestrCs par des cellules (qui contiennent aussi souvent des granules de mClanine) situCes dans les vaisseaux sanguins (pas dans les sinusoi'des) du paren...

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Studies on the reticulo-endothelial system of the dogfish, Scyliorhinus canicula

Cited by 13 publications

References 27 publications

Migratory, respiratory burst and mitogenic responses of leucocytes isolated from the gills of rainbow trout (Oncorhynchus mykiss)

Migratory, respiratory burst and mitogenic responses of leucocytes isolated from the gills of rainbow trout (Oncorhynchus mykiss)

Are thrombocytes and platelets true phagocytes?

Studies on the reticuloendothelial system of the dogfish Scyliorhinus canicula. Endocytic activity of fixed cells in the spleen and liver

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