STUDIES ON THE HISTOCHEMICAL DISTRIBUTION OF OXIDATIVE ENZYMES IN <i>TRICHOMONAS VAGINALIS</i>

Sharma, Narsingh N.; Bourne, Geoffrey H.

doi:10.1177/11.5.628

Cited by 18 publications

(5 citation statements)

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“…The existence of lysosomes in trichomonads has been repeatedly inferred from histochemical observations on granular staining of hydrolases (8,36,37) and from the sedimentability and latency of acid phosphatase and hyaluronidase (1,12,29) . In view of the complex picture of the distribution of hydrolases in T. foetus, further comparative studies are needed to understand the function of the different activity .…”

Section: Localization Of Oxidoreductasesmentioning

confidence: 98%

Biochemical Cytology of Trichomonad Flagellates

Müller

1973

The Journal of Cell Biology

103

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To determine the localization of several enzymes in Tritrichomonas foetus, the axenic KV-1 strain was grown in Diamond's medium with bovine serum, homogenized in 0 .25 M sucrose, and subjected to analytical differential and isopycnic centrifugation . The fractions were assayed for their enzymatic composition and examined electron microscopically . NADH and NADPH dehydrogenases, about 90% of the catalase, and two hydrolases, agalactosidase and manganese-activated ß-galactosidase I are in the nonsedimentable part of the cytoplasm . a-Glycerophosphate and malate dehydrogenases are associated with a large particle, whose equilibrium density in sucrose gradients is 1 .24 . This particle corresponds to that population of the paracostal and paraxostylar granules which, having a uniform granular matrix surrounded by a single membrane, resemble microbodies from other organisms . The small sedimentable portion of catalase (about 10% of the total activity) is not associated with these granules and equilibrates at density 1 .22 . The nature of the subcellular entity carrying catalase could not be ascertained . Hydrolases with a pH optimum around 6-6 .5 (protease, ß-N-acetylglucosaminidase, ß-N-acetylgalactosaminidase, and cation-independent ß-galactosidase II), as well as a large part of acid phosphatase, are associated with a population of large particles which equilibrate at densities from 1 .15 to 1 .20 . The hydrolases in these granules lose their structure-bound latency easily after freezing and thawing . These particles correspond to another population of the paracostal and paraxostylar granules which have varied shape and inhomogeneous content with frequent myelin figures, indicating a digestive function . The rest of the phosphatase and most of the acid ß-glucuronidase activity are in a smaller granule fraction with an equilibrium density around 1 .18 . The latency of these enzymes is quite resistant to freezing and thawing . This particle population consists of smaller, very often flattened vesicles and granules, many of which are clearly fragments of the prominent Golgi apparatus of the cell .

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Section: Localization Of Oxidoreductasesmentioning

confidence: 98%

Biochemical Cytology of Trichomonad Flagellates

Müller

1973

The Journal of Cell Biology

103

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“…Amoebida (Entamoeba species), Diplomonadida, Trichomonadida, Hypermas tigina, Holotricha, and Oligotricha of the rumen of Bovidae and of the coecum of Equidae. There are, however, microbody-like structures present in several of these groups that were assumed, on the basis of positive histochemical reactions for dehydrogenases and with DAB,to represent redox organelles in trichomonads (28,98,104) and hypermastiginids (59). Biochemical data are available only for tri chomonads.…”

Section: Anaerobic Protozoamentioning

confidence: 99%

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

Müller¹

1975

Annu. Rev. Microbiol.

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“…They could likely serve in organellogenesis of the costa and possibly supply energy for contraction of the Costa. Evidence supporting the energy source concept was pub lished by Sharma and Bourne (1963 in which they reported the presence of Krebs cycle enzymes in the subcostal granules. These granules were found to be PAS-posltive by Abraham and Honigberg (1964) and by Sharma and Honigberg (1966), lending additional weight to this con cept.…”

Section: Discussionmentioning

confidence: 92%

Ultrastructure of porcine trichomonads from culture

Townsend

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STUDIES ON THE HISTOCHEMICAL DISTRIBUTION OF OXIDATIVE ENZYMES IN TRICHOMONAS VAGINALIS

Cited by 18 publications

References 0 publications

Biochemical Cytology of Trichomonad Flagellates

Biochemical Cytology of Trichomonad Flagellates

BIOCHEMISTRY OF PROTOZOAN MICROBODIES: Peroxisomes, α-Glycerophosphate Oxidase Bodies, Hydrogenosomes

Ultrastructure of porcine trichomonads from culture

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