Studies on the chaotropically solubilized arylsulfatase C and estrone sulfatase of sheep brain

Lakshmi, Sreeja; Balasubramanian, A.S.

doi:10.1016/0005-2744(79)90185-2

Cited by 14 publications

(5 citation statements)

References 15 publications

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“…The apparent K m value (3 × 10 -6M) was in the same order as has been reported for microsomal estrone sulfatases from the rat and the guinea pig (3,4), although higher K m values have also been reported (5). The mode of action of tamoxifen upon the sulfatase activity is not known.…”

Section: Discussionsupporting

confidence: 73%

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Estradiol formation and estrogen receptors in breast tumor tissue: Effects of tamoxifen on estrogen interconversions in breast tumor homogenate in vitro

Wilking

Carlström

Sköldefors

et al. 1984

Breast Cancer Res Tr

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Section: Discussionsupporting

confidence: 73%

“…The similarity to noncompetitive inhibition indicates binding of tamoxifen to a site of the enzyme different from the catalytic site. It is known that certain low molecular compounds, obviously not participating in the reaction as nucleophiles, enhance the activity of hydrolytic enzymes (5,6).…”

Section: Discussionmentioning

confidence: 99%

Estradiol formation and estrogen receptors in breast tumor tissue: Effects of tamoxifen on estrogen interconversions in breast tumor homogenate in vitro

Wilking

Carlström

Sköldefors

et al. 1984

Breast Cancer Res Tr

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“…After incubation for 1 h at 37°C the reaction was stopped by adding 1 ml of 0.1 M-Na,CO,. The liberated estrone was extracted thrice with 4 rnl ether, dried and counted in a Beckman LS-100 liquid scintillation counter, as described by Lakshmi and Balasubramanian (1979).…”

Section: Assuy Of Enzymesmentioning

confidence: 99%

“…Arylsulphatase C was assayed as described earlier (Lakshmi and Balasubramanian, 1979), based on the method of Milsom et al (1972).…”

Section: Assuy Of Enzymesmentioning

confidence: 99%

The Distribution of Estrone Sulphatase, Dehydroepiandrosterone Sulphatase, and Arylsulphatase C in the Primate (Macaca radiata) Brain and Pituitary

Lakshmi

Balasubramanian

1981

Journal of Neurochemistry

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Estrone sulphatase, arylsulphatase C and dehydroepiandrosterone sulphatase were measured in the pituitary, hypothalamus-preoptic area, amygdala, hippocampus, midbrain, septum, frontal cortex and occipital cortex of monkey (Macaca radiata) brain. All the regions showed measurable activities of all three enzymes. In all the animals tested, either the midbrain or hypothalamus-preoptic area showed the greatest activity of all three enzymes. In particular, estrone sulphatase showed the highest specific activity in either of the above two regions in all animals. Subcellular distribution studies in the hypothalamus preoptic area showed a similarity in the distribution profile between arylsulphatase C and estrone sulphatase and a significant difference of dehydroepiandrosterone sulphatase from the others.

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“…We have recently reported the presence of estrogen sulfatase in fetal ovine brain tissue throughout the last trimester of gestation [10]. In other studies, the presence of sulfatase has already been detected in brain tissue from adult sheep [11, 12], rats [13, 14, 15], primates [16], and human beings [17]. …”

Section: Introductionmentioning

confidence: 99%

Ovine Fetal Estrogen Sulfotransferase in Brain Regions Important for Hypothalamus-Pituitary-Adrenal Axis Control

Purinton

Wood

2000

Neuroendocrinology

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Ovine parturition is initiated by increases in fetal hypothalamus-pituitary-adrenal (HPA) axis activity which, in turn, increase placental estrogen biosynthesis and ultimately increase uterine contractility. In addition to their action in the uterus, estrogens in fetal plasma augment fetal corticotropin (ACTH) secretion. In late gestation, estrone sulfate is more abundant in fetal plasma than unconjugated estrone and it is possible that there is interconversion of sulfoconjugated and unconjugated steroids within the fetal brain. We studied hypothalamus and brainstem tissue from fetal, neonatal, and adult sheep to test the hypothesis that the ovine brain contains estrogen sulfotransferase. Although no significant ontogenic pattern was revealed, the presence of estrogen sulfotransferase within the hypothalamus and brainstem was detectable. Immunohistochemistry revealed the presence of estrogen sulfotransferase in the paraventricular nucleus of the hypothalamus, the nucleus of the tractus solatarius, and the rostral ventral lateral medulla. We conclude that ovine fetal hypothalamus and brainstem contain estrogen sulfotransferase in brain regions important for HPA axis control.

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Studies on the chaotropically solubilized arylsulfatase C and estrone sulfatase of sheep brain

Cited by 14 publications

References 15 publications

Estradiol formation and estrogen receptors in breast tumor tissue: Effects of tamoxifen on estrogen interconversions in breast tumor homogenate in vitro

Estradiol formation and estrogen receptors in breast tumor tissue: Effects of tamoxifen on estrogen interconversions in breast tumor homogenate in vitro

The Distribution of Estrone Sulphatase, Dehydroepiandrosterone Sulphatase, and Arylsulphatase C in the Primate (Macaca radiata) Brain and Pituitary

Ovine Fetal Estrogen Sulfotransferase in Brain Regions Important for Hypothalamus-Pituitary-Adrenal Axis Control

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