The Distribution of Estrone Sulphatase, Dehydroepiandrosterone Sulphatase, and Arylsulphatase C in the Primate (Macaca radiata) Brain and Pituitary

Abstract

Estrone sulphatase, arylsulphatase C and dehydroepiandrosterone sulphatase were measured in the pituitary, hypothalamus-preoptic area, amygdala, hippocampus, midbrain, septum, frontal cortex and occipital cortex of monkey (Macaca radiata) brain. All the regions showed measurable activities of all three enzymes. In all the animals tested, either the midbrain or hypothalamus-preoptic area showed the greatest activity of all three enzymes. In particular, estrone sulphatase showed the highest specific activity in either of the above two regions in all animals. Subcellular distribution studies in the hypothalamus preoptic area showed a similarity in the distribution profile between arylsulphatase C and estrone sulphatase and a significant difference of dehydroepiandrosterone sulphatase from the others.

Arylsulphatase C and Estrone Sulphatase of Sheep Hypothalamus, Preoptic Area, and Midbrain: Separation by Hydrophobic Interaction Chromatography and Evidence for Differences in Their Lipid Environment

“…McNought and France (1980) have suggested that membrane alterations are responsible for placental steroid sulphatase deficiency. Further studies would be needed to identify the topological distribution of arylsulphatase C and estrone sulphatase on the microsomal and nuclear membranes where most of their activity is localised (Dolly et al, 1972;Lakshmi and Balasubramanian, 1981).…”

“…After incubation at 37°C for 2 h, 0.8 ml ethanol and 0.5 ml 1 M KOH were added, centrifuged at 0-4°C and the liberated p-nitrophenol measured at 400 nm in a Carl Zeiss PMQ I1 spectrophotometer (Milsom et al, 1972). The reaction mixture for estrone sulphatase was as described earlier (Lakshmi and Balasubramanian, 1981) except for the use of 1.4 x lo5 countshin estrone sulphate and up to 0.15 ml enzyme. The liberated estrone was measured according to Iwamori e t al.…”

“…The liberated estrone was measured according to Iwamori e t al. (1976), using a toluene-ethanol-based scintillation fluid (Lakshmi and Balasubramanian, 1981). The enzyme activity was linear with respect to time in all the assays.…”

Ovine Fetal Estrogen Sulfotransferase in Brain Regions Important for Hypothalamus-Pituitary-Adrenal Axis Control

“…We have recently reported the presence of estrogen sulfatase in fetal ovine brain tissue throughout the last trimester of gestation [10]. In other studies, the presence of sulfatase has already been detected in brain tissue from adult sheep [11, 12], rats [13, 14, 15], primates [16], and human beings [17]. …”