STUDIES ON PROTEIN METABOLISM OF THE CORNEA: 2. THE INCORPORATION OF C¹⁴-LABELED AMINO ACIDS INTO COMPONENTS OF THE RABBIT CORNEA

Abstract

Preliminary studies were made on the incorporation of C14-labeled glycine and proline into tissue cultures of rabbit stromal cells, excised rabbit corneas, and rabbit corneas in vivo. Some evidence has been presented that this incorporation reflects synthesis, chiefly into proteins. With rabbit corneas in vitro, no evidence was found that the presence of epithelium increased the incorporation of amino acid into constituents of the stroma. During in vivo incorporation it was found that the proteins of the endothelium were about twice and the proteins of the epithelium about four times as active as those of the stroma.