Studies on liquefaction and storage of ejaculated dromedary camel (Camelus dromedarius) semen

Wani, Nisar Ahmad; Billah, M.; Skidmore, J.A.

doi:10.1016/j.anireprosci.2007.10.011

Cited by 43 publications

(37 citation statements)

References 22 publications

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“…Moreover, in accordance with the present results, Wani et al (2008) found that the proportion of dromedary camel oocytes fertilized with epididymal spermatozoa was 43% and those that developed to blastocysts was 14%. Whereas, using freshly ejaculated semen, the rate of cleavage in dromedary camel ranged from 51 to 64% and the rate of development to blastocyst ranged from 16.5% to 23% (Khatir et al 2005).…”

Section: Discussionsupporting

confidence: 79%

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Abdel-Khalek¹,

Gabr²,

Khalil³

et al. 2017

Polish Journal of Veterinary Sciences

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Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter). Meanwhile, Fetal Dromedary Camel Serum (FDCS) was obtained from camel fetuses after slaughtering. Thereafter, only Cumulus Oocyte Complexes (COCs) were matured in vitro in the Tissue Culture Medium (TCM-199) complemented with 10% FDCS. Spermatozoa required for in vitro fertilization were collected from testes (epididymal cauda) of the slaughtered camel bulls. The results clearly showed that the maturation rate of oocytes at metaphase II was about 59.5% while the fertilization rate was around 70.4%. Intriguingly, the embryo rates determined were 13.1%, in 2-cell; 0.0%, in 4-cell; 34.7%, in 8-16% cell; 39.1%, in morula and 13.1% in a blastocyst stage. This study represented a successful in vitro production of Sudanese dromedary camel embryos from epididymal sperm cells and in vitro matured oocytes recovered from slaughtered camels.

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Section: Discussionsupporting

confidence: 79%

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Abdel-Khalek¹,

Gabr²,

Khalil³

et al. 2017

Polish Journal of Veterinary Sciences

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“…The semen therefore has a low concentration of 30–150 × 10 6 spermatozoa/ml in alpacas and llamas and 80–300 × 10 6 spermatozoa/ml in camels despite the low seminal plasma volume (Deen et al. 2003; Wani et al. 2008).…”

Section: Semen Characteristicsmentioning

confidence: 99%

Seminal Plasma Components in Camelids and Comparisons with Other Species

Kershaw-Young

Maxwell

2012

Reprod Domestic Animals

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Contents Camelid semen is characterized by a highly viscous, low‐volume ejaculate with a low concentration of spermatozoa that exhibit low progressive motility. The viscous seminal plasma is currently the major impediment to the development of assisted reproductive technologies (ARTs) in camelids. To advance ARTs such as sperm cryopreservation and artificial insemination in camelids, it is necessary to identify the cause of the viscosity and gain an understanding of the role of seminal plasma components on sperm function and fertility. Numerous compounds and proteins have been identified as mediators of sperm function and predictors of fertility in other livestock species, and understanding the importance of specific proteins has progressed the success of ARTs in these species. Current knowledge on the components of camelid seminal plasma is outlined, together with the implications of these components for the development of ARTs in camelids. The cause of semen viscosity, as well as proteins that are present in camelid seminal plasma, is described for the first time. Seminal plasma components are compared with those of other species to hypothesize their role in sperm function and fertility.

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“…Whilst 384 11% lactose has been reported to be the optimal extender for liquid or frozen storage of camelid 385 sperm (Morton et al 2007;Wani et al 2008) other studies report that tris-based extenders 386 2007). In order toTo fully benefit from the optimised method for seminal plasma viscosity 397 reduction protocol using papain and E-64 it is necessary to systematically and thoroughly 398 investigate the effect of all semen extender components on the integrity and function of alpaca 399 sperm during and after cryopreservation.…”

Section: Experiments 1: Optimisation Of Papain Concentration and Time 253mentioning

confidence: 99%

Papain and its inhibitor E-64 reduce camelid semen viscosity without impairing sperm function and improve post-thaw motility rates

Kershaw

Evans

Rodney

et al. 2017

Reprod. Fertil. Dev.

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In camelids, the development of assisted reproductive technologies is impaired by the viscous nature of the semen. The protease papain has shown promise in reducing viscosity, although its effect on sperm integrity is unknown. The present study determined the optimal papain concentration and exposure time to reduce seminal plasma viscosity and investigated the effect of papain and its inhibitor E-64 on sperm function and cryopreservation in alpacas. Papain (0.1 mg mL–1, 20 min, 37°C) eliminated alpaca semen viscosity while maintaining sperm motility, viability, acrosome integrity and DNA integrity. Furthermore E-64 (10 µM at 37°C for 5 min after 20 min papain) inhibited the papain without impairing sperm function. Cryopreserved, papain-treated alpaca spermatozoa exhibited higher total motility rates after chilling and 0 and 1 h after thawing compared with control (untreated) samples. Papain treatment, followed by inhibition of papain with E-64, is effective in reducing alpaca seminal plasma viscosity without impairing sperm integrity and improves post-thaw motility rates of cryopreserved alpaca spermatozoa. The use of the combination of papain and E-64 to eliminate the viscous component of camelid semen may aid the development of assisted reproductive technologies in camelids.

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Studies on liquefaction and storage of ejaculated dromedary camel (Camelus dromedarius) semen

Cited by 43 publications

References 22 publications

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Seminal Plasma Components in Camelids and Comparisons with Other Species

Papain and its inhibitor E-64 reduce camelid semen viscosity without impairing sperm function and improve post-thaw motility rates

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