In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Abdel-Khalek, A. E.; Gabr, Sh. A.; Khalil, Wael A.; Shamiah, Sh. M.; Pan, Li-Chern; Qin, Guangsheng; Farouk, Mohammed Hamdy

doi:10.1515/pjvs-2017-0013

Cited by 11 publications

(8 citation statements)

References 20 publications

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“…Our results showed the same. In this study, The present studythe rates of camel oocyte in vitro maturation showed similar or higher capacity when compared with other studies, meaning that our camel IVM system was appropriate [22][23][24], and analysis revealed thea greater efficiency in obtaining enhanced maturation ratesmatured oocytes from of in vivo-matured oocytes compare to in vitro-sources [Table 1]. Indeed, we confirmed the enhanced capacities for cleavage and blastocyst stage formation of in vivo-matured oocytes.…”

Section: A C C E P T E Dsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

“…These results indicated that it is essential to choose an efficient oocyte maturation method to obtain stable results in the production of live births using SCNT. However, the oocyte maturation rate of camels was lower than that of other species after IVM, and studies were insufficient [ 22 – 24 ]. According to recent studies, camels are generally slaughtered when they are too old or too young before their maturity; therefore, they do not undergo normal reproduction cycles and have been reported to have reduced embryo development capacities [ 1 , 6 ].…”

Section: Discussionmentioning

confidence: 99%

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Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Son¹,

Jeong²,

Jeong³

et al. 2022

Anim Biosci

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Objective: The present study evaluated the efficiency of embryo development and clinical pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos were produced by SCNT using in vivo-and in vitromatured oocytes. In vitro embryo developmental capacity of reconstructed embryos was evaluated. To confirm the efficiency of pregnancy and live birth rates, a total of 72 blastocysts using in vitromatured oocytes transferred into 45 surrogates and 95 blastocysts using in vivo-matured oocytes were transferred into 62 surrogates by transvaginal method. Results:The collected oocytes derived from ovum pick up (OPU) showed higher maturation potential into metaphase II oocytes than oocytes from the slaughterhouse. The competence of cleavage, and blastocyst were also significantly higher in in vivo-matured oocytes than in vitro-matured oocytes.After embryo transfer, 11 pregnant and 10 live births were confirmed in in vivo-matured oocytes group, and 2 pregnant and 1 live birth were confirmed in in vitro-matured oocytes group. Furthermore, blastocysts produced by in vivo-matured oocytes resulted in significantly higher early pregnancy and live birth rates than in vitro-matured oocytes. Conclusion:In this study, SCNT embryos using in vivo-and in vitro-matured camel oocytes were successfully developed, and pregnancy was established in recipient camels. We also confirmed that in vivo-matured oocytes improved the development of embryos and the pregnancy capacity using the blastocyst embryo transfer method.

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Section: A C C E P T E Dsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Son¹,

Jeong²,

Jeong³

et al. 2022

Anim Biosci

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“…However, the low reproductive performance of camels is considered an important factor affecting camel populations. Employing in vitro embryo production (IVP) technologies is an efficient means of propagating genetically superior camels; however, compared to other livestock species, camel oocyte in vitro maturation (IVM) has been reported to be inefficient [1,2,3,4]. One of the main contributing factors is the limited availability of oocytes because the majority of the slaughtered animals are either old, culled for infertility, or very young and have not attained maturity; in addition, there is no standardized protocol for IVP in camels [3, 5].…”

mentioning

confidence: 99%

Effects of all-trans retinoic acid on the in vitro maturation of camel (Camelus dromedarius) cumulus-oocyte complexes

Saadeldin

Swelum

Elsafadi

et al. 2019

Journal of Reproduction and Development

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All- trans retinoic acid (RA) is a metabolite of vitamin A and has pleiotropic actions on many different biological processes, including cell growth and differentiation, and is involved in different aspects of fertility and developmental biology. In the current study, we investigated the effects of RA on camel ( Camelus dromedarius ) cumulus-oocyte complex in vitro maturation (IVM). IVM medium was supplemented with 0, 10, 20, and 40 µM RA. Application of 20 µM RA significantly reduced the proportion of degenerated oocytes and significantly improved oocyte meiosis and first polar body extrusion compared to the control and other experimental groups. Retinoic acid significantly reduced the mRNA transcript levels of apoptosis-related genes, including BAX and P53 , and reduced the BAX/BCL2 ratio. In addition, RA significantly reduced the expression of the Transforming growth factor beta (TGFβ) pathway-related transcripts associated with the actin cytoskeleton, ACTA2 and TAGLN ; however, RA increased TGFβ expression in cumulus cells. The small molecule SB-431542 inhibits the TGFβ pathway by inhibiting the activity of activin receptor-like kinases (ALK-4, ALK-5, and ALK-7); however, combined supplementation with RA during IVM compensated for the inhibitory effect of SB-431542 on cumulus expansion, oocyte meiosis I, and first polar body extrusion in activated oocytes. The current study shows the beneficial effects of RA on camel oocyte IVM and provides a model to study the multifunctional mechanisms involved in cumulus expansion and oocyte meiosis, particularly those involved in the TGFβ pathway.

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“…Ovaries of camels collected from Afak slaughterhouse and transport by cool box contain 0.9% Normal saline (20-25Cº) supplement with 100 IU penicillin and 100 µg/ml streptomycin (23). Oocytes collected by one of the following techniques…”

Section: Methodsmentioning

confidence: 99%

Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes

Al-Malikey¹,

Al-Delemi²

2021

AJVS

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This study aimed to know the effect of vitamin C on in vitro oocytes maturation of Iraqi she-camel with different techniques collection. Several oocytes collection technique have been used: Three hundred ninety oocytes were collected from 84 ovaries from Afak slaughterhouse within half to one hour of slaughter animal and transport by cool box contain normal saline 0.9% (20-25C°) to laboratory of Al-Diwaniyah veterinary Hospital within 1-2 hours. After washing the ovaries with normal saline, each 28 ovaries: oocytes collected by one of the following techniques: Aspiration, slicing, and dissection. Oocytes collected from the three techniques counted and graded. Only grades A and B were selected, and undergo in maturation process. and matured in maturation medium (M199-A) and incubated in CO2 incubator at 5% CO2, 38.5 Cº, and 90% humidity for 24h and supplement the media with 0, 25, 50, 100 µg/ml of vit.C for each technique. Aspirated oocytes were cultured the results was expended cumulus cells was 66.66% (66/99) and the appearance first polar body (F.P.B.) was 68.1% (45/66). Maturation medium supplement with 50µg/ml of vit. C higher rate (84% & 81.8 expended cumulus and F.P.B.) than other groups with significant (P<5%). Maturation oocytes by slicing technique were cultured, expended cumulus was 61.4% (59/96) and the appearance F.P.B. 62.7% (37/59). Maturation medium supplement with 50µg/ml of vit. C (79.16 & 73.68% expended cumulus and F.P.B.) higher rate than other groups with significant (P<5%). Maturation oocytes by dissection technique were cultured. expended cumulus was 57.2% (59/103) and the appearance F.P.B. 55.93% (33/59). Maturation medium supplement with 50µg/ml of vit. C higher rate than other groups with significant (P<5%).

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In vitro production of Sudanese camel (Camelus dromedarius) embryos from epididymal spermatozoa and follicular oocytes of slaughtered animals

Cited by 11 publications

References 20 publications

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes

Effects of all<i>-trans</i> retinoic acid on the <i>in vitro</i> maturation of camel (<i>Camelus dromedarius</i>) cumulus-oocyte complexes

Effect of Vitamin C on In Vitro Maturation of Iraqi She-Camel Oocytes

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