Studies on lipolytic molds. Comparative study of lipases obtained from molds grown on oil seeds

Abstract

Summary A. niger andA. flavus isolated from oil seed source show an appreciable amount of lipolytic activity. The optimum pH of the mold lipase varies to a certain extent with the nature of the substrate and buffer used. In the experiments carried out the optimum buffer concentration is 2 cc., the optimum substrate concentration is 1–2 cc., and the optimum temperature is 35–37°C. for hydrolysis of peanut oil by mold lipases. Sodium taurocholate, managanese sulphate, KH2PO4, sodium acetate, etc., accelerate the activity of the mold lipase. The change in activity of acetone‐dried sample of mold lipase with time is negligible. The synthetic activity of the mold lipase is higher than that of seed and cake lipases. A definite relation between the vitamin contents and the lipolytic activity of the molds could not be found. Peanut cake medium appears to be a good source to grow the lipolytic molds on a large scale.