Distribution of oil or other diethyl ether extractable material in various fractions of peanuts infected with selected fungi

Abstract

Perpentage oil or other di~thyl ether soluble material was determined in lydphilized ground whole seeds a~l~,:~rt;buffer _(~odium.~phosphate; pH 7.9, I -0.pl) soluble and insoluble ~xtracts of high quality "Floru~ner" peanuts (Arachia hypogaea L.) not inoculated or inoculated witl~ Aspergillus parasiticus, A~pergilIus oryzae, Rhizopus! oligosporus, or Neurospbra sitophila and held for ,~arious time intervals to 1~ days, During the test p~riod, percentage ether e~tractable material fncrease¢ in buffer soluble frac-tiOn~ of ,,,peanuts infected Jwitt~ these fungi and decreased in insoluble pr~eparatibns. The trend~ of these quantitative changes at various test inte~lil~ ~ere.similar for see.ds infected with A. par~igc~s .a~d seeds infected with N. sitophila and A. oryzae~,:,T, l!o percentage ether soluble material in whole seeds infected with th~ese fungi.either decreased slightly or dig not chang6 durmgtl~ete~ pen-ods. Dala ~t/~ s fl~tFtech~i~iies 'deqetoIS~'d ' tO -~Op~irate-o11-hh~t~0i}Ot 'i extraction processes may fiO~g~ sdftable;fOt~N01~fiiig these components from molded or partially decomposed seeds.

Colonization and Biochemical Changes in Peanut Seeds Infected with Aspergillus flavus1

“…Induced biochemical changes of this nature result in reduction of dry matter and oil, increase in free fatty acids and in eventual deterioration of seed quality and nutritional value of infested peanuts (11,13). Studies conducted by Cherry et al (4,6,7,8,9,10) on the effect of Aspergilli (A flavus, A. parasiticus, and A. oryzae) and other fungi (Neurospora sitophila and Rhizopus oligosporus) have shown the following changes that were distinguishable from "standard" profiles of uninoculated peanuts: Decomposition of the major storage proteins to small molecular weight components and quantitative depletion of the small protein components (4,6,7); changes in enzyme activity (10); changes in total amino acids (8); and changes in quantity of ether-soluble oil (9). Other reports (2,5,12) have shown the production of aflatoxin when peanuts are invaded by fungi.…”