Studies on healthy and neoplastic tissues using positron annihilation lifetime spectroscopy and focused histopathological imaging

Abstract

Samples of healthy and neoplastic myometrial tissues were investigated using Positron Annihilation Lifetime Spectroscopy (PALS). Meaningful differences between normal and diseased tissues were observed for each patient. The differences were also clearly visible for various kinds of lesions in each patient. The set of lifetimes and intensities obtained from PALS was correlated with the histopathological examinations of the same fragments of tissues. Strong coincidence between PALS parameters and histopathological findings was observed only in the case of a very precise correlation of the investigated area in both techniques. Measurements and discussion presented here were carried out to develop a method for measuring the sub-nanometric structure of human tissues. This kind of investigation, using positron probe, creates an opportunity of a new application in Positron Emission Tomography (PET).

Hybrid total-body pet scanners—current status and future perspectives

“…Not only oncology, but also cardiology and neurology are likely to benefit from these developments. From a technical point of view, the increased sensitivity along with maintained TOF performance and SNR could lead to the manifestation of new detection and reconstruction techniques, such as positron annihilation lifetime spectroscopy (PALS), allowing to discriminate between healthy and malignant tissues [ 71 , 122 ]. For PALS, the exact reconstruction of the point of annihilation is necessary to assign the lifetime of a positronium atom allocated in void tissue space before its decay to the respective tissue.…”

Simple and Eco-Friendly Route from Agro-Food Waste to Water Pollutants Removal

“…3415 cm −1 , which corresponds to oxygen-containing functional groups accessible for RhB with abundant adsorption sites (Figure 11). This indicates the formation of H-bonds in the CCP/RhB system [43,87]. A weak intensity band at 3065 cm −1 belongs to aromatic C-H vibrations, which also disappeared after adsorption RhB into the CCP [88].…”

Positronium Physics and Biomedical Applications

“…Positronium annihilation in tissues strongly depends on water content. In highly hydrated organs (lens) or tissues (myoma), the mean o-Ps lifetime is below or around ∼2 ns (Sane et al, 2010;Zgardzinska et al, 2020). In adipose tissue this time is significantly increased (Avachat et al, 2022;Moskal et al, 2021a,b) confirming the observation from biological systems that structural characteristic and molecular composition determine positronium annihilation.…”

“…Further studies on complex biological systems like biological membranes or proteins moved positronium research towards structural biology and showed that this approach was efficient in the study of biological reactions (such as electron transfer (Jean and Ache, 1977)), phase transition of lipids (Chow et al, 1981;Jean and Hancock, 1982;Stinson et al, 1980) macromolecule structure (Handel et al, 1976), hydratation (Akiyama et al, 2007Gregory et al, 1992;Handel et al, 1980) and porosity (Chamerski et al, 2017;Pamula et al, 2006) of biological samples. Nowadays, after many years of focused research, PALS appears to be a promising technique in the investigation of the structure of macromolecules (Chen et al, 2012) and clinical samples (Avachat et al, 2022;Moskal et al, 2021a,b;Zgardzinska et al, 2020).…”