Studies on glycogen-induced inflammation of mice

Abstract

The intraperitoneal injection of glycogen in the mouse resulted, shortly thereafter, in the accumulation of 14-23 million neutrophils in the peritoneal cavity and a four-fold increase in the numbers of circulating neutrophils. Preceding the influx of leukocytes, the exudation of plasma proteins and the chemotactic activity for mouse neutrophil in vitro increased in the peritoneal fluid. Among various protease inhibitors examined, chymostatin alone suppressed the plasma protein exudation. Indomethacin and dexamethasone reduced the accumulation of white cells and protein exudation. These nonsteroidal and steroidal antiinflammatory drugs were equally effective whether given simultaneously with or 60 min before glycogen or whether administered intraperitoneally or orally. Colchicine showed a suppressive effect on the leukocyte accumulation but enhanced the protein exudation.

Peritoneal and head kidney cell response to intraperitoneally injected yeast glucan in Atlantic salmon, Salmo salar L.

“…For fish injected with FIA or glycogen the neutrophils predominated in the initial leueoeyte response, while maerophages characterized the response to yeast glucan. The relative number of leueoeytes may possibly also be influenced by the concentration of inflammatory agent used (Yamashita et al 1982).…”

“…The ability oi leucocytes to mobilize quickly at the sites of injury serves to minimize the spread of disease and to initiate the immune response. The duration, cell number and cell composition of the leucocyte response varies with the type of material injected (Finn Sc Nielson 1971;Yamashita et al 1982;MacArthur et al 1984). The peritoneal cell response in fish is especially interesting because immunomodulators as well as vaccines are frequently administered by i.p.…”

“…injection of yeast glucan, glycogen and Freund's incomplete adjuvant (FIA). Glycogen was chosen because it has been used to elicit sterile, peritoneal inflammatory cell exudates in both mammals and fish (Yamashita et al 1982;MacArthur et al 1984), and FIA was chosen because it is composed of mineral oils, a material which is frequently used as an adjuvant in vaccines for mammals as well as fish (Cossarini-Dunier 1985;Adams, Auchinaehie, Bundy, Tatner & Home 1988). The leucocyte cell composition of the head kidney after i.p.…”

Phenolic Compounds Isolated fromCalea unifloraLess. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)

“…After this period, the animals were anesthetized and euthanized with an overdose of pentobarbital (120 mg/kg) administered subcutaneously (s.c.). The cells were collected by rinsing the abdominal cavity with 3 mL of sterile PBS, pH 7.6 [21].…”

“…Neutrophils were obtained from the fluid leakage of the mouse peritoneal lavage, washed with PBS (pH 7.6), and the mixture was centrifuged at 900 × g for 10 min at 4°C (Sorvall™ ST 40, Thermo Scientific®, Swedesboro, NJ, USA). After washing, the neutrophils were suspended in PBS (pH 7.6) and 30 μ L of this cell suspension (1 × 10 6 ) was incubated with 10 μ L of different doses of noreugenin (NRG: 1, 2.5, 5, 10, 50, or 100 μ M) or α -hydroxy-butein (AH-BU: 1, 2.5, 5, 10, 50, or 100 μ M) in the presence or absence of lipopolysaccharide (LPS: 5 μ g/mL, 10 μ L) (LPS from Escherichia coli serotype O111:B4, Sigma-Aldrich, St. Louis, MO, USA), for 18 h in an enzyme immunoassay (EIE) plate [21–23]. For the next step, 10 μ L of neutrophil suspension from each sample of the different doses of the compounds was mixed with 10 μ L of trypan blue dye solution (0.4%).…”