Studies on classification of eel leucocytes.

Kusuda, Riichi; Ikeda, Yûji

doi:10.2331/suisan.53.205

Cited by 14 publications

(7 citation statements)

References 3 publications

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“…The presence of eosinophils in the blood of teleosts has been suggested by several light-and electron-microscopic studies (Catton 1951;Williams and Warner 1976;Bielek 1981;Cenini 1984;Ishizeki et al 1984;Zuasti and Ferrer 1988;Meseguer et al 1990;López-Ruiz et al 1992). However, other authors (Klontz 1972;Blaxhall and Daisley 1973;Ellis 1976;Ferguson 1976;Cannon et al 1980;Nakamura and Shimozawa 1984;Kusuda and Ikeda 1987) have been unable to find these cells. Barber and Mills Westermann (1978) have examined the peripheral blood of 58 species of fish, mostly teleosts, and observed eosinophils in only four of them.…”

Section: Introductionmentioning

confidence: 91%

Response of eosinophilic granule cells of gilthead seabream ( Sparus aurata , Teleostei) to bacteria and bacterial products

Noya

Lamas

1996

Cell and Tissue Research

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Eosinophilic granule cells in the gills and peritoneal exudate of gilthead seabream (Sparus aurata L.) are characterized by the presence of prominent eosinophilic granules in their cytoplasm and are here described for the first time. The oval granules of these cells contain an electron-dense inclusion surrounded by a less dense filamentous matrix and are peroxidase- and acid phosphatase-negative. Unlike other granulocytes of gilthead seabream, eosinophilic granule cells do not ingest bacteria in vivo. The intraperitoneal injection of extracellular products of Pasteurella piscicida induces mobilization of eosinophilic granule cells to the blood and other tissues and causes changes in their structure. Shortly after injection, the granules of eosinophilic granule cells become swollen and some fuse with the cell membrane. From 7 h post-injection, many eosinophilic granule cells in the gills degenerate and are then phagocytosed by macrophages, which are especially abundant after 24 h. From 24 h to 72 h, eosinophilic granule cells from the gills contain abundant autolysosomes together with granules of a normal morphology.

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Section: Introductionmentioning

confidence: 91%

Response of eosinophilic granule cells of gilthead seabream ( Sparus aurata , Teleostei) to bacteria and bacterial products

Noya

Lamas

1996

Cell and Tissue Research

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“…dulus majalis, and Cyprinodon uariegatus (Gardner and Yevich, 1969) or described as the single granulocyte type present in other fish species (Weinberg et al, 1972;Mattisson and Fange, 1977;Barber and Westermann, 1978;Cannon et al, 1980;Groman, 1982;Kusuda and Ikeda, 1987). The nucleus of S. aurata neutrophils could be described as polymorphic but, according to Ellis (1977) the term "polymorphonuclear leukocyte," used to designate their mammalian counterpart, would be inappropriate.…”

Section: Figs 17-21mentioning

confidence: 99%

“…Some authors describe a single lymphocyte type or lymphocyte-like cell (Weinberg et al, 1972;Williams and Warner, 1976;Elarifi, 1982;Hightower et al, 1984;Roubal, 1986;Kusuda and Ikeda, 1987) but usually both small and large lymphocytes representing different cell maturation stages are observed (Barber and Westermann, 1975;Etlinger et al, 1976;Ellis, 1977;Christensen et al, 1978;Cannon et al, 1980). In S. aurata we have characterized small and large lymphocytes showing ultrastructural features similar to those present in other vertebrates, mainly fish (Blaxhall, 1983;Savage, 1983;Cenini, 1984;Temmink and Bayne, 1987;Doggett and Harris, 1989;Fujimaki and Isoda, 1990).…”

Section: Lymphocytesmentioning

confidence: 99%

Blood cells of the gilthead seabream (Sparus aurata L.): Light and electron microscopic studies

1992

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The peripheral blood cells of the gilthead seabream (Sparus aurata L.) were studied by light and transmission and scanning electron microscopic methods. Acidophilic erythroblasts and mature erythrocytes, round, oval, and fusiform thrombocytes, neutrophils, acidophils and basophils, lymphocytes, plasma cells, and monocyte-macrophages were characterized. A comparison of our light and electron microscopic results was carried out. The results were discussed with those for other fish species and the main modifications from the common vertebrate haematological pattern observed being considered.

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“…Staphylococcus aureus can be phagocytized in vitro by thrombocytes in the exudate of carp Cyprinus carpio (Suzuki 1986), as can Edwardsiella tarda in Japanese eel Anguilla japonica (Kusuda & Ikeda 1987 Matushima & Mariano (1996) and Martins et al (2006) confirmed the defense function of thrombocytes. In this trial, the fish receiving the supplemented diet presented a greater number of neutrophils than did the non-supplemented fish, regardless of vaccination.…”

Section: Discussionmentioning

confidence: 91%

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

Salvador¹,

Toazza²,

Moraes³

et al. 2012

Dis. Aquat. Org.

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This study evaluated the effects of dietary supplementation with 0.3% Saccharomyces cerevisiae yeast cell wall and of vaccination against Streptococcus agalactiae on the cellular component of acute inflammation induced in the coelomic cavity of Nile tilapia Oreochromis niloticus and on survival of the fish after challenge. A total of 84 tilapia of mean (± SD) weight 125.0 ± 1.5 g were distributed among twelve 310 l fiberglass tanks according to a 2 × 2 × 3 factorial design in the following manner: with and without supplementation; 2 stimulations (oily solution without S. agalactiae vaccine and vaccination); 15 d later all fish were intracoelomically challenged with 10 8 CFU ml -1 of a homologous strain of S. agalactiae, and evaluated after 6, 24 and 48 h, with 7 replicates. The fish received the non-supplemented or supplemented diet for a total of 77 d. The vaccination was performed on the 60th day, intracoelomically, as a single injection of 0.5 ml of the vaccine containing 10 8 CFU ml −1. Fifteen days later, all the fish were challenged with S. agalactiae by means of an intracoelomic inoculation of 10 8 CFU ml −1. No mortality was observed among the supplemented fish. The fish that were fed the non-supplemented diet and immunized with the bacterium presented a mortality rate of 28.5%. Among the non-supplemented and nonimmunized fish, the mortality rate was 38.09%. Supplementation, in both vaccinated and nonvaccinated fish, induced larger accumulations of thrombocytes, lymphocytes and macrophages at the inflammatory focus. The results suggest that supplementation with 0.3% yeast cell wall, in both vaccinated and non-vaccinated fish, improved the in flammatory response of the fish and protected against the challenge. Vaccination increased the defense response, but the effect was stronger when associated with supplementation with S. cerevisiae. KEY WORDS: Inflammation · Saccharomyces cerevisiae · Vaccination · Streptococcus agalactiae · ChallengeResale or republication not permitted without written consent of the publisher

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Studies on classification of eel leucocytes.

Cited by 14 publications

References 3 publications

Response of eosinophilic granule cells of gilthead seabream ( Sparus aurata , Teleostei) to bacteria and bacterial products

Response of eosinophilic granule cells of gilthead seabream ( Sparus aurata , Teleostei) to bacteria and bacterial products

Blood cells of the gilthead seabream (Sparus aurata L.): Light and electron microscopic studies

Inflammatory responses of Nile tilapia Oreochromis niloticus to Streptococcus agalactiae: effects of vaccination and yeast diet supplement

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