Studi Viabilitas Serbuk Sari Pada Salak Bangkalan

Setiawan, Eko

doi:10.21107/rekayasa.v12i1.5299

Cited by 5 publications

(5 citation statements)

References 14 publications

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“…Female palms are the second best salacca breeders needed since the plants produce fruit which is the most utilized part of the plant. Breeders do not want too many male palms because they are only needed as pollen donors on salacca pollination and will not produce fruits (Setiawan 2019). 2015).…”

Section: Resultsmentioning

confidence: 99%

Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss)

PRIHATINI

DINARTI²,

SUTANTO³

et al. 2023

Biodiversitas

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Abstract. Prihatini R, Dinarti D, Sutanto A, Sudarsono. 2023. Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss). Biodiversitas 24: 704-712. Salacca zalacca (Gaertn.) Voss is a native tropical fruit species of Indonesia with three different sex types, i.e., female, male, and hermaphrodite. Early sex identification plays a vital role in the dioecious plant development, cultivation, and breeding program. The morphological and histological markers cannot differentiate salacca sex types during vegetative stages, during which molecular approaches are offered. Isolation of salacca's partial Transducin Beta Like-3 (TBL3) gene was done using Phoenix dactylifera L. TBL3-specific primers. After DNA sequencing and multiple sequence alignment, the putative TBL3 aligned data was used to construct a phylogenetic tree, find SNAP primers, and use the generated markers as predictors for sex-type phenotypes of salacca. Phylogenetic analysis showed that the dendrogram of aligned TBL3 partial sequences of salacca and other taxa showed that the salacca TBL3 is closely related to P. dactylifera and Elaeis guineensis Jacq. since the three palms belong to the same Arecaceae family. The partial sequence of the TBL3 gene of hermaphrodite salacca revealed ten single nucleotide polymorphisms (SNPs) compared to those of female and male plants. Protein alignment of the sequence also showed several differences, which may lead to different flower morphogenesis. The salacca SNAP sex-types markers were developed from one of the SNP sites and validated using samples from farmer plantations. The SNAP primer pairs of STBL1F-Ref - STBL1-Rev amplified a 300 bp fragment from hermaphrodite samples, whereas no band was amplified from the female and male samples. On the contrary, primers STBL1F-Alt - STBL1-Rev amplified a 300 bp fragment from female and male plants but not from the hermaphrodite plants. The salacca's TBL3 sequences and the SNAP markers are valuable tools for further salacca genomic exploration.

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Section: Resultsmentioning

confidence: 99%

Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss)

PRIHATINI

DINARTI²,

SUTANTO³

et al. 2023

Biodiversitas

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“…The result showed that the percentage of fertile pollen of Grobogan and Anjasmoro reached 100% (Figure 3.). Fertile male salak's pollen showed a change in color (colored) after being dropped with 1% lugol's solution [2].…”

Section: Pollen Fertilitymentioning

confidence: 99%

“…Soybean is also a shortday plant so it can initiate flowering [1]. Soybeans usually flowering at 25-40 days [2].…”

Section: Introductionmentioning

confidence: 99%

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Increased of soybean genetic variability through hand pollination

Hanafiah

Haryati

DAMANIK

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Soybean is one of the most popular cropin Indonesia. In the last five years, soybean production has decreased. Therefore, one way to increase the productivity and genetic variability of soybean is hand pollination. This study aims to see and observe the flower and the fertility of soybean pollen and to know the correct stages of crossing. This study was conducted by observing the morphology and physiology of Grobogan and Anjasmoro soybeans. Parameters observed were flower parts, crossing stage, and pollen fertility. The result of this study showed that Grobogan and Anjasmoro were monoecious. In the fertility test, several samples found that Grobogan and Anjasmoro flowers were 100% fertile. In performing hand pollination, there are several stages, as follows; the selection of male and female flowers, castration, emasculation, pollination, isolation, and labeling. Crosses of varieties between Grobogan and Anjasmoro have good enough success rate, which is equal 57.14%.

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“…Salak Pondoh is one of the Salak varieties from Yogyakarta, which has a super quality compared to other varieties (Lestari et al 2013) (Figure 1). Fresh Salak from Indonesia has been exported to several countries as Singapore, Malaysia, Hong Kong, and China (Lestari et al 2011;Setiawan 2019). One of the efforts to increase fruit formation was im-proving the plant quality and internal physiology by providing organic chemical compounds or plant growth regulators (PGRs) (Bons, Kaur 2020).Gibberellin (GA3) is used as a plant growth regulators (PGR) to control the process of fruit formation (Kaur 2017).…”

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confidence: 99%

The combination of plant growth regulators (GA3 and Gracilaria sp. extract) and several fertilisers in Salak Pondoh fruit production

Prihastanti¹,

Haryanti²

2022

Hort. Sci. (Prague)

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Plant growth regulators (PGRs) and proper fertilisers are used to increase quality of the Salak Pondoh, such as in the plant production, fruit number and size. This study aims to determine the type and dosage of PGRs and the best type of fertiliser to increase the production bunch and fruit of Salak Pondoh. The method used was a Factorial Completely Randomised Design (FCRD) with 36 treatments (excluding the control) and 3 replications. Three treatments were conducted: first, as a control, no PGR treatment + fertilisation (no fertilisation, manure, and NPK). Second, a 3 × 3 factorial scheme. Treatment of PGR GA3 (50, 100, 150 ppm) + fertilisation (no fertilisation, manure, and NPK). Third, a 3 × 3 × 3 factorial scheme was used. Treatment of PGR Gracilaria sp. extract (50, 100, 150 ppm) with different solvents (n-Hexane, methylene-chloride, ethanol) + fertilisation (no fertilisation, manure, and NPK). The parameters observed were the number length, area of the bunch, length and area of the fruit. The results showed the highest number of bunches was found in PGR GA3 50 ppm. While, the highest bunch area was found in PGR Gracilaria sp. ethanol 150 ppm. The PGRs only increased the formation of the bunches and did not increase the length and width of the bunches and the fruit size. Fertilisation affected the Salak production in the form of the number, length and width of the bunches, and the fruit size. In addition, the fertiliser increased the number of the bunches, bunch area, and length and area of the fruit. The interaction of the PGR and fertilisation had no significant effect on increasing the Salak Pondoh production. The best result in increasing the Salak Pondoh production was PGR Gracilaria sp. ethanol 100 ppm with manure fertilisation.

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Studi Viabilitas Serbuk Sari Pada Salak Bangkalan

Cited by 5 publications

References 14 publications

Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss)

Sex-linked Single Nucleotide Polymorphism (SNP) identification and molecular marker development of salacca (Salacca zalacca (Gaertn.) Voss)

Increased of soybean genetic variability through hand pollination

The combination of plant growth regulators (GA3 and Gracilaria sp. extract) and several fertilisers in Salak Pondoh fruit production

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