Structure and properties of hybrid biopolymer particles fabricated by co-precipitation cross-linking dissolution procedure

Xiong, Yu; Georgieva, Radostina; Steffen, Axel; Smuda, Kathrin; Bäumler, Hans

doi:10.1016/j.jcis.2017.12.030

Cited by 20 publications

(32 citation statements)

References 57 publications

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“…However, the zeta potential of HA-d-HbMPs and HA-t-HbMPs became significantly more negative (increased by 36%-38%) compared to HbMP (Figure 3B). Similar results are reported in our previous studies about the influence of HA on MnCO 3 -templated Hb/BSA-HA particles [30]. The enhanced zeta potential values of the HA-d-HbMPs and HA-t-HbMPs confirm the attachment of HA, a strongly negatively charged polysaccharide, on the surface of the particles.…”

Section: Zeta Potentialsupporting

confidence: 91%

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Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

et al. 2019

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Hemoglobin-based oxygen carriers (HBOCs) represent a propitious type of blood substitute to transport oxygen throughout the body while acting as a carrier in biomedical applications. However, HBOCs in blood are recognized and rapidly scavenged by the body’s innate immune systems. To overcome this problem, HBOCs require a surface modification that provides protection against detection and elimination in order to prolong their circulation time after administration. In this study, we investigated different surface modifications of hemoglobin submicron particles (HbMPs) by double/triple precipitation, as well as by adsorption of human serum albumin (HSA), hyaluronic acid (HA), and pluronic (Plu) to discover how diverse surface modifications influence the oxygen binding capacity and the binding of anti-hemoglobin (Hb) antibodies, immunoglobulin G (IgG), and haptoglobin (HP) to HbMPs. The particle size and zeta potential of the six types of HbMP modifications were analyzed by zeta sizer, confocal laser scanning microscopy, and transmission electron microscopy (TEM), and were compared to the unmodified HbMPs. The results revealed that all surface-modified HbMPs had a submicron size with a negative charge. A slight decrease in the oxygen binding capacity was noticed. The specific binding of anti-Hb antibodies, IgG, and HP to all surface-modified HbMPs was reduced. This indicates a coating design able to protect the particles from detection and elimination processes by the immune system, and should lead to a delayed clearance and the required and essential increase in half-life in circulation of these particles in order to fulfill their purpose. Our surface modification method reflects a promising strategy for submicron particle design, and can lead the way toward novel biomedical applications.

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Section: Zeta Potentialsupporting

confidence: 91%

“…HbMPs were fabricated using the highly promising CCD technique based on MnCO 3 , which has been shown to be very simple and effective for entrapment of proteins into submicron particles [21,25,30,37].…”

Section: Binding Of Haptoglobin To Surface-modified Hbmpsmentioning

confidence: 99%

Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

et al. 2019

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“…The immobilization of compounds is a promising strategy for the improvement of stability, solubility, and biological activity through compound capture by carbonate microspheres in the process of their formation (Co-precipitation). The Co-precipitation Crosslinking Dissolution technique (CCD-technique) resulted in the fabrication of biopolymer particles using the precipitation of MnCl 2 and Na 2 CO 3 in the presence of a biopolymer solutions [14,15]. In the case of proteins, we used glutaraldehyde to crosslink the proteins in the MnCO 3 template.…”

Section: Introductionmentioning

confidence: 99%

Albumin Submicron Particles with Entrapped Riboflavin—Fabrication and Characterization

et al. 2019

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Although riboflavin (RF) belongs to the water-soluble vitamins of group B, its solubility is low. Therefore, the application of micro-formulations may help to overcome this limiting factor for the delivery of RF. In this study we immobilized RF in newly developed albumin submicron particles prepared using the Co-precipitation Crosslinking Dissolution technique (CCD-technique) of manganese chloride and sodium carbonate in the presence of human serum albumin (HSA) and RF. The resulting RF containing HSA particles (RF-HSA-MPs) showed a narrow size distribution in the range of 0.9 to 1 μm, uniform peanut-like morphology, and a zeta-potential of −15 mV. In vitro release studies represented biphasic release profiles of RF in a phosphate buffered saline (PBS) pH 7.4 and a cell culture medium (RPMI) 1640 medium over a prolonged period. Hemolysis, platelet activation, and phagocytosis assays revealed a good hemocompatibility of RF-HSA-MPs.

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“…The results of flow cytometry analysis in Figure 3 also demonstrated that Odex-particles had no auto-fluorescence in contrast to the protein particles prepared using the CCD technique and crosslinked by glutaraldehyde [8][9][10]29,30].…”

Section: Particle Characterisationmentioning

confidence: 85%

In-vitro haemocompatibility of dextran-protein submicron particles

Kloypan

Suwannasom

Chaiwaree

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Blood compatibility is a key requirement to fulfil for intravenous administration of drug and oxygen carrier system. Recently, we published the fabrication of oxidised-dextran (Odex)-crosslinked protein particles by one-pot formulation. In the current study we investigate the haemocompatibility of these Odex-particles including albumin particles (Odex-APs) and haemoglobin particles (Odex-HbMPs). Odex-APs and Odex-HbMPs have a submicron size ranged 800-1000 nm with peanut-like shape and a negative surface charge. In vitro haemocompatibility assays included haemolysis test, indirect phagocytosis test and platelet activation test in human blood. Odex-APs and Odex-HbMPs did not provoke any undesirable effects on the blood cells. Firstly, the ratio of haemolysis after contacted with Odex-crosslinked protein particles were less than 5% and therefore the particles may be considered non-haemolytic. Secondly, the incubation of leukocyte with Odex-APs/HbMPs did not influence the phagocytosis of leukocyte. We conclude that our particles are not recognized by monocytes or granulocytes. Finally, exposure of Odex-APs/HbMPs to platelets did not cause an activation of platelets. Additionally, Odex-HbMP/AP did not enhance or attenuate agonist-induced platelet activation. We conclude that Odex-crosslinked protein particles exhibit a very good haemocompatibility and represent highly promising carriers for drugs or oxygen.

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Structure and properties of hybrid biopolymer particles fabricated by co-precipitation cross-linking dissolution procedure

Cited by 20 publications

References 57 publications

Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

Surface Modification of Hemoglobin-Based Oxygen Carriers Reduces Recognition by Haptoglobin, Immunoglobulin, and Hemoglobin Antibodies

Albumin Submicron Particles with Entrapped Riboflavin—Fabrication and Characterization

In-vitro haemocompatibility of dextran-protein submicron particles

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