In-vitro haemocompatibility of dextran-protein submicron particles

Abstract

Blood compatibility is a key requirement to fulfil for intravenous administration of drug and oxygen carrier system. Recently, we published the fabrication of oxidised-dextran (Odex)-crosslinked protein particles by one-pot formulation. In the current study we investigate the haemocompatibility of these Odex-particles including albumin particles (Odex-APs) and haemoglobin particles (Odex-HbMPs). Odex-APs and Odex-HbMPs have a submicron size ranged 800-1000 nm with peanut-like shape and a negative surface charge. In vitro haemocompatibility assays included haemolysis test, indirect phagocytosis test and platelet activation test in human blood. Odex-APs and Odex-HbMPs did not provoke any undesirable effects on the blood cells. Firstly, the ratio of haemolysis after contacted with Odex-crosslinked protein particles were less than 5% and therefore the particles may be considered non-haemolytic. Secondly, the incubation of leukocyte with Odex-APs/HbMPs did not influence the phagocytosis of leukocyte. We conclude that our particles are not recognized by monocytes or granulocytes. Finally, exposure of Odex-APs/HbMPs to platelets did not cause an activation of platelets. Additionally, Odex-HbMP/AP did not enhance or attenuate agonist-induced platelet activation. We conclude that Odex-crosslinked protein particles exhibit a very good haemocompatibility and represent highly promising carriers for drugs or oxygen.

Integrated endotoxin-adsorption and antibacterial properties of platelet-membrane-coated copper silicate hollow microspheres for wound healing

“…7 A). Additionally, the hemolysis rate of a nanomaterial must be less than 5% to ensure safety during intravenous administration [ 63 , 64 ]. As shown in Additional file 1 : Figure S12, the hemolysis rate for 500 μg mL −1 CSO@PM (i.e., 10-times the concentration applied in vivo to treat infected wounds) is less than 5%.…”

Function of Hemoglobin-Based Oxygen Carriers: Determination of Methemoglobin Content by Spectral Extinction Measurements

“…For SEM, the samples must be dried and the particles are covered by a thin platinum layer. Hence, due to dehumidification shrinking of the HbMPs might be induced [17]. On the other hand, AFM images are taken in solution approximately matching the biological environment for the intended application.…”

Künstliche Sauerstofftransporter können mehr als Sauerstoff liefern

“…B. mittels Glutaraldehyds. Die beiden Schritte der Präzipitation und Vernetzung lassen sich bei Verwendung von oxidiertem Dextran zu einem Schritt zusammenfassen [89,90]. Die schwammartigen HbMP sind das Endergebnis nach der Auflösung des anorganischen Carbonat-Templates.…”