Albumin Submicron Particles with Entrapped Riboflavin—Fabrication and Characterization

Suwannasom, Nittiya; Smuda, Kathrin; Kloypan, Chiraphat; Kaewprayoon, Waraporn; Baisaeng, Nuttakorn; Prapan, Ausanai; Chaiwaree, Saranya; Georgieva, Radostina; Bäumler, Hans

doi:10.3390/nano9030482

Cited by 8 publications

(13 citation statements)

References 43 publications

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“…The uniform peanut-like particles showed a narrow size distribution in the range of 0.9 to 1 µm and a negative zeta-potential. The RF-albumin submicron particles revealed a good hemocompatibility [116].…”

Section: Rf Encapsulationmentioning

confidence: 95%

“…In addition, the stability of alginate-chitosan was measured over a five-month period at 4 • C in solution. Demonstrating a simple coprecipitation method of albumin submicron particles with good biocompatibility 900 ± 1000 nm [116] Jin et al [113] encapsulated RF using modified soy protein/dextran with the range of 143.3 nm size. The EE% and LC values of nanogels were up to 65.9% and up to 12%, respectively.…”

Section: Rf Encapsulationmentioning

confidence: 99%

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Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

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225

107

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Riboflavin (RF) is a water-soluble member of the B-vitamin family. Sufficient dietary and supplemental RF intake appears to have a protective effect on various medical conditions such as sepsis, ischemia etc., while it also contributes to the reduction in the risk of some forms of cancer in humans. These biological effects of RF have been widely studied for their anti-oxidant, anti-aging, anti-inflammatory, anti-nociceptive and anti-cancer properties. Moreover, the combination of RF and other compounds or drugs can have a wide variety of effects and protective properties, and diminish the toxic effect of drugs in several treatments. Research has been done in order to review the latest findings about the link between RF and different clinical aberrations. Since further studies have been published in this field, it is appropriate to consider a re-evaluation of the importance of RF in terms of its beneficial properties.

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Section: Rf Encapsulationmentioning

confidence: 95%

Section: Rf Encapsulationmentioning

confidence: 99%

Riboflavin: The Health Benefits of a Forgotten Natural Vitamin

Suwannasom

Kao

Pruß

et al. 2020

IJMS

Self Cite

225

107

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“…Therefore, the adsorption of NS into the particles might be dependent on the ratio of particle templates and NS concentration that is available for adsorption. As we know from a previous study involving fabricated particles by using the CCD-technique, MnCO 3 particle template displayed the potential to entrap poor water solubility such as riboflavin compound and these particles can carry an amount of riboflavin in water four times higher than the solubility of just riboflavin alone [ 18 ]. Furthermore, in a study involving curcumin as a hydrophobic molecule, it was found to be capable of being entrapped in the hydrophobic cavity of HSA nanoparticles [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…Both particles demonstrated a submicron size with a weak level of autofluorescence from the glutaraldehyde. Generally, the size of the particles depends upon certain precipitation factors such as the type and concentration of the salt, temperature, pH, mixing speed, reaction time, and any additives [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…The incorporation of hydrophobic compounds into HSA particles can increase their degree of stability, solubility, and biological activity. HSA MPs can be fabricated by the coprecipitation crosslinking dissolution technique (CCD-technique) [ 17 , 18 , 19 ]. Firstly, coprecipitation is performed by mixing HSA-containing MnCl 2 and Na 2 CO 3 solutions.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication and Characterization of Human Serum Albumin Particles Loaded with Non-Sericin Extract Obtained from Silk Cocoon as a Carrier System for Hydrophobic Substances

et al. 2021

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Non-sericin (NS) extract was produced from the ethanolic extract of Bombyx mori silk cocoons. This extract is composed of both carotenoids and flavonoids. Many of these compounds are composed of substances of poor aqueous solubility. Thus, this study focused on the development of a carrier system created from biocompatible and biodegradable materials to improve the biological activity of NS extracts. Accordingly, NS was incorporated into human serum albumin template particles with MnCO3 (NS-HSA MPs) by loading NS into the preformed HAS-MnCO3 microparticles using the coprecipitation crosslinking dissolution technique (CCD-technique). After crosslinking and template dissolution steps, the NS loaded HSA particles are negatively charged, have a size ranging from 0.8 to 0.9 µm, and are peanut shaped. The degree of encapsulation efficiency ranged from 7% to 57% depending on the initial NS concentration and the steps of adsorption. In addition, NS-HSA MPs were taken up by human lung adenocarcinoma (A549 cell) for 24 h. The promotion of cellular uptake was evaluated by flow cytometry and the results produced 99% fluorescent stained cells. Moreover, the results from CLSM and 3D fluorescence imaging confirmed particle localization in the cells. Interestingly, NS-HSA MPs could not induce inflammation through nitric oxide production from macrophage RAW264.7 cells. This is the first study involving the loading of non-sericin extracts into HSA MPs by CCD technique to enhance the bioavailability and biological effects of NS. Therefore, HSA MPs could be utilized as a carrier system for hydrophobic substances targeting cells with albumin receptors.

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