Fabrication and Characterization of Human Serum Albumin Particles Loaded with Non-Sericin Extract Obtained from Silk Cocoon as a Carrier System for Hydrophobic Substances

Abstract

Non-sericin (NS) extract was produced from the ethanolic extract of Bombyx mori silk cocoons. This extract is composed of both carotenoids and flavonoids. Many of these compounds are composed of substances of poor aqueous solubility. Thus, this study focused on the development of a carrier system created from biocompatible and biodegradable materials to improve the biological activity of NS extracts. Accordingly, NS was incorporated into human serum albumin template particles with MnCO3 (NS-HSA MPs) by loading NS into the preformed HAS-MnCO3 microparticles using the coprecipitation crosslinking dissolution technique (CCD-technique). After crosslinking and template dissolution steps, the NS loaded HSA particles are negatively charged, have a size ranging from 0.8 to 0.9 µm, and are peanut shaped. The degree of encapsulation efficiency ranged from 7% to 57% depending on the initial NS concentration and the steps of adsorption. In addition, NS-HSA MPs were taken up by human lung adenocarcinoma (A549 cell) for 24 h. The promotion of cellular uptake was evaluated by flow cytometry and the results produced 99% fluorescent stained cells. Moreover, the results from CLSM and 3D fluorescence imaging confirmed particle localization in the cells. Interestingly, NS-HSA MPs could not induce inflammation through nitric oxide production from macrophage RAW264.7 cells. This is the first study involving the loading of non-sericin extracts into HSA MPs by CCD technique to enhance the bioavailability and biological effects of NS. Therefore, HSA MPs could be utilized as a carrier system for hydrophobic substances targeting cells with albumin receptors.

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

“…The results reveal that the submicron particles displayed a "hairy" surface. Also, it is seen that, despite the addition of CUR, a typical peanut-like structure was formed by the porous MnCO 3 template, as previously found in our studies using the CCD technique [26][27][28][29].…”

“…An effective submicron particle fabrication technique, namely co-precipitation-cross-linking-dissolution (CCD), has been established to produce biopolymer particles [24][25][26]. In this method, biopolymer particles are produced via co-precipitation, by simply mixing MnCl 2 and Na 2 CO 3 solutions in the presence of albumin [26,27]. This approach has been shown to have high effectiveness for entrapping various biopolymers (e.g., proteins and hemoglobin) into carbonate particles before dissolving with EDTA to obtain pure biopolymer submicron particles.…”

Potentiality of Melittin-Loaded Niosomal Vesicles Against Vancomycin-Intermediate Staphylococcus aureus and Staphylococcal Skin Infection

“…The uptake of FITC-labeled Mel-loaded NISVs was visualized by 3D holotomographic microscopy according to Jantakee et al, 43 with some modifications. A375 cells (5×10 4 cells) were seeded in a 50 mm Tomo dish and incubated at 37°C in a humidified atmosphere of 5% CO 2 until they reached 70% confluence.…”