Structure and N-acetylglucosamine binding of the distal domain of mouse adenovirus 2 fibre

Singh, Abhimanyu; Nguyen, Thanh H.; Vidovszky, Márton Z.; Harrach, Balázs; Benkő, Mária; Kirwan, Alan; Joshi, Lokesh; Kilcoyne, Michelle; Berbís, M. Álvaro; Cañada, F. Javier; Jiménez‐Barbero, Jesús; Menéndez, Margarita; Wilson, Sarah S.; Bromme, Beth A.; Smith, Jason G.; Raaij, M.J. van

doi:10.1099/jgv.0.001145

Cited by 8 publications

(9 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies with mouse CAR‐transfected B16 melanoma cells did not reveal increased MAdV‐2 or ‐3 infection (M. Bieri and S. Hemmi, unpublished). This is consistent with the fact that few of the residues important for CAR binding are conserved in the MAdV‐2 fiber head . Competition studies with recombinant MAdV‐2 fiber knob demonstrated that fiber knob is the attachment protein , paralleling competition results for MAdV‐1 fiber knob discussed above .…”

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptsupporting

confidence: 84%

“…This is consistent with the fact that few of the residues important for CAR binding are conserved in the MAdV‐2 fiber head . Competition studies with recombinant MAdV‐2 fiber knob demonstrated that fiber knob is the attachment protein , paralleling competition results for MAdV‐1 fiber knob discussed above . However, the receptors for MAdV‐1 and MAdV‐2 on cultured cells are not the same, because purified MAdV‐2 fiber knob does not inhibit MAdV‐1 infection .…”

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptsupporting

confidence: 80%

“…However, the RGD motif present in the fiber knob of MAdV‐1 and ‐3 is lacking in the fiber knob of MAdV‐2, which has little sequence identity (10–16%) to AdV fibers of known structures. A high‐resolution crystal structure of the carboxy‐terminus of the MAdV‐2 fiber reveals a domain with the typical AdV fiber head topology and a domain containing two triple β‐spiral repeats of the shaft domain . The fiber head contains a monosaccharide N‐acetylglucosamine (GlcNAc) binding site that allows binding to GlcNAc‐containing mucin glycans, potentially representing a target in the mouse gut.…”

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptmentioning

confidence: 99%

See 2 more Smart Citations

Murine adenoviruses: tools for studying adenovirus pathogenesis in a natural host

Hemmi

Spindler

2019

FEBS Letters

View full text Add to dashboard Cite

Small laboratory animals are powerful models for investigating in vivo viral pathogenesis of a number of viruses. For adenoviruses (AdVs), however, species-specificity poses limitations to studying human adenoviruses (HAdVs) in mice and other small laboratory animals. Thus, this review covers work on naturally occurring mouse AdVs, primarily mouse adenovirus type 1 (MAdV-1), a member of the species Murine mastadenovirus A. Molecular genetics, virus life cycle, cell and tissue tropism, interactions with the host immune response, persistence, and host genetics of susceptibility are described. A brief discussion of MAdV-2 (member of species Murine mastadenovirus B) and MAdV-3 (member of species Murine mastadenovirus C) is included. We report the use of MAdVs in the development of vectors and vaccines.

show abstract

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptsupporting

confidence: 84%

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptsupporting

confidence: 80%

Section: Madv‐1 Pathogenesis—persistence and Host Genetics Of Susceptmentioning

confidence: 99%

See 1 more Smart Citation

Murine adenoviruses: tools for studying adenovirus pathogenesis in a natural host

Hemmi

Spindler

2019

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Neoglycoconjugate (NGC) microarrays were printed essentially as previously described (Singh et al, 2018;Flannery et al, 2020). The microarrays were printed in eight replicate subarrays per microarray slide consisting of 52 different NGCs and glycoproteins (probes) in replicates of six probes per subarray (Table S1).…”

Section: Construction Of Neoglycoconjugate Microarrays and Interrogation For Bacterial Bindingmentioning

confidence: 99%

“…Two-step incubations were carried out in triplicate. After incubation, washing and drying, microarray slides were scanned immediately (Singh et al, 2018). As additional controls, microarray incubations with fluorescence-labelled secondary antibodies anti-goat and anti-rabbit (1:1,000 dilution in TBS-T) were also performed.…”

Section: Construction Of Neoglycoconjugate Microarrays and Interrogation For Bacterial Bindingmentioning

confidence: 99%

Burkholderia cenocepacia BCAM2418‐induced antibody inhibits bacterial adhesion, confers protection to infection and enables identification of host glycans as adhesin targets

Pimenta

Kilcoyne

Bernardes

et al. 2021

Cellular Microbiology

Self Cite

View full text Add to dashboard Cite

Trimeric Autotransporter Adhesins (TAA) found in Gram-negative bacteria play a key role in virulence. This is the case of Burkholderia cepacia complex (Bcc), a group of related bacteria able to cause infections in patients with cystic fibrosis. These bacteria use TAAs, among other virulence factors, to bind to host protein receptors and their carbohydrate ligands. Blocking such contacts is an attractive approach to inhibit Bcc infections. In this study, using an antibody produced against the TAA BCAM2418 from the epidemic strain Burkholderia cenocepacia K56-2, we were able to uncover its roles as an adhesin and the type of host glycan structures that serve as recognition targets. The neutralisation of BCAM2418 was found to cause a reduction in the adhesion of the bacteria to bronchial cells and mucins. Moreover, in vivo studies have shown that the anti-BCAM2418 antibody exerted an inhibitory effect during infection in Galleria mellonella. Finally, inferred by glycan arrays, we were able to predict for the first time, host glycan epitopes for a TAA. We show that BCAM2418 favoured binding to 3 0 sialyl-3-fucosyllactose, histo-blood group A, α-(1,2)-linked Fuccontaining structures, Lewis structures and GM1 gangliosides. In addition, the glycan microarrays demonstrated similar specificities of Burkholderia species for their most intensely binding carbohydrates. K E Y W O R D S adhesin antibody, Burkholderia cepacia complex, host glycans as adhesin targets, trimeric autotransporter adhesins 1 | INTRODUCTION Members of the denominated Burkholderia cepacia complex (Bcc; group of 22 species), can cause severe infections in humans and animals (Scoffone et al., 2017). Among those, Burkholderia cenocepacia and Burkholderia multivorans are particularly dangerous for cystic fibrosis (CF) patients (Scoffone et al., 2017). They are highly adapted

show abstract

Mucin Purification and Printing Natural Mucin Microarrays

Berre

Gerlach

Gallagher

et al. 2022

Methods in Molecular Biology

View full text Add to dashboard Cite

Structure and N-acetylglucosamine binding of the distal domain of mouse adenovirus 2 fibre

Cited by 8 publications

References 65 publications

Murine adenoviruses: tools for studying adenovirus pathogenesis in a natural host

Murine adenoviruses: tools for studying adenovirus pathogenesis in a natural host

Burkholderia cenocepacia BCAM2418‐induced antibody inhibits bacterial adhesion, confers protection to infection and enables identification of host glycans as adhesin targets

Mucin Purification and Printing Natural Mucin Microarrays

Contact Info

Product

Resources

About