Mucin Purification and Printing Natural Mucin Microarrays

Berre, Marie Le; Gerlach, Jared Q.; Gallagher, Mary E.; Joshi, Lokesh; Carrington, Stephen D.; Kilcoyne, Michelle

doi:10.1007/978-1-0716-2148-6_8

Cited by 2 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The insoluble precipitate (crude mucin) was collected and purified further by Caesium chloride (CsCl) density gradient centrifugation method. 45 Briefly, the crude mucin was diluted with 10 mM sodium phosphate buffer ( pH 6.5) containing 6 M guanidine hydrochloride (GuHCl) and adjusted to a density of 1.2 g ml −1 with CsCl and centrifuged (55 000 rpm at 10.0 °C for 62 h in Beckman Coulter, |Avanti centrifuge J-30). Aliquots of 0.5 ml were sampled, the absorption at 280 nm was measured, and 2 μL of each fraction was spotted and stained with Alcian blue.…”

Section: 27mentioning

confidence: 99%

Effect of beta glucan coating on controlled release, bioaccessibility, and absorption of β-carotene from loaded liposomes

Niaz,

Mackie

2024

Food Funct.

View full text Add to dashboard Cite

show abstract

Section: 27mentioning

confidence: 99%

Effect of beta glucan coating on controlled release, bioaccessibility, and absorption of β-carotene from loaded liposomes

Niaz,

Mackie

2024

Food Funct.

View full text Add to dashboard Cite

show abstract

Opening the doors of precision medicine: novel tools to assess intestinal barrier in inflammatory bowel disease and colitis-associated neoplasia

Iacucci,

Santacroce,

Majumder

et al. 2024

Gut

View full text Add to dashboard Cite

Mounting evidence underscores the pivotal role of the intestinal barrier and its convoluted network with diet and intestinal microbiome in the pathogenesis of inflammatory bowel disease (IBD) and colitis-associated colorectal cancer (CRC). Moreover, the bidirectional association of the intestinal barrier with the liver and brain, known as the gut-brain axis, plays a crucial role in developing complications, including extraintestinal manifestations of IBD and CRC metastasis. Consequently, barrier healing represents a crucial therapeutic target in these inflammatory-dependent disorders, with barrier assessment predicting disease outcomes, response to therapy and extraintestinal manifestations.New advanced technologies are revolutionising our understanding of the barrier paradigm, enabling the accurate assessment of the intestinal barrier and aiding in unravelling the complexity of the gut-brain axis. Cutting-edge endoscopic imaging techniques, such as ultra-high magnification endocytoscopy and probe-based confocal laser endomicroscopy, are new technologies allowing real-time exploration of the ‘cellular’ intestinal barrier. Additionally, novel advanced spatial imaging technology platforms, including multispectral imaging, upconversion nanoparticles, digital spatial profiling, optical spectroscopy and mass cytometry, enable a deep and comprehensive assessment of the ‘molecular’ and ‘ultrastructural’ barrier. In this promising landscape, artificial intelligence plays a pivotal role in standardising and integrating these novel tools, thereby contributing to barrier assessment and prediction of outcomes.Looking ahead, this integrated and comprehensive approach holds the promise of uncovering new therapeutic targets, breaking the therapeutic ceiling in IBD. Novel molecules, dietary interventions and microbiome modulation strategies aim to restore, reinforce, or modulate the gut-brain axis. These advancements have the potential for transformative and personalised approaches to managing IBD.

show abstract

Mucin Purification and Printing Natural Mucin Microarrays

Cited by 2 publications

References 10 publications

Effect of beta glucan coating on controlled release, bioaccessibility, and absorption of β-carotene from loaded liposomes

Effect of beta glucan coating on controlled release, bioaccessibility, and absorption of β-carotene from loaded liposomes

Opening the doors of precision medicine: novel tools to assess intestinal barrier in inflammatory bowel disease and colitis-associated neoplasia

Contact Info

Product

Resources

About