Structurally Characterized Zn2+ Selective Ratiometric Fluorescence Probe in 100 % Water for HeLa Cell Imaging: Experimental and Computational Studies

Kumari, Babli; Lohar, Sisir; Ghosh, M.; Ta, Sabyasachi; Banerjee, Prajna; Chattopadhyay, Ansuman

doi:10.1007/s10895-015-1688-9

Cited by 16 publications

(13 citation statements)

References 52 publications

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“…Gram character of the isolate HB01 was assayed using Gram staining technique (Smibert and Krieg 1994). For preliminary shape and size analysis, freshly growing cells on nutrient broth (NB) (Himedia ® ) were treated with zinc acetate, followed by Zn-binding ligand (Kumari et al 2016). Then, these were observed using fluorescence microscopy (Leica DM1000, Germany) at × 100.…”

Section: Bacteriological Analysismentioning

confidence: 99%

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

Halder

Banerjee

Biswas

et al. 2020

Environmental Sustainability

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Production of green pigments has drawn great attention recently in various applications like foods, cosmetics, nutraceuticals, and pharmaceuticals as it counters the harmful effects of synthetic dyes. Use of green natural products, especially microbederived colours in food and cosmetics is rapidly developing for its eco-friendly nature and cost effectiveness. In this study, a red pigment producing rod-shaped, Gram-negative bacterium Serratia rubidaea HB01 was isolated from domestic sewage. The isolate exhibited wide resistance to various groups of antibiotics, including Aminoglycosides, Lincosamides, Macrolides, Nitroimidazoles, Penicillins, Quinolones, and Tetracyclines. The isolate S. rubidaea HB01 significantly produced red prodigiosin pigment (17.9 mg ml −1). The production of prodigiosin was highly pH sensitive and growth media specific. The UV-Vis spectrum of the red pigment dissolved in methanol showed a maximum absorption at 536 nm in neutral pH. Red fraction with R f value 0.38 purified by thin layer chromatography (TLC) was further subjected to liquid chromatography and mass spectrometric (LC-MS) analysis. Moreover, a strong band of pyrrolenine (C = N) at v max 1639 was found comparable to prodigiosin as observed from Fourier-transform infrared (FTIR) spectra. Alongside, the presence of a typical methoxy group with chemical shifts at δ 4.0 ppm and δ 58.5 ppm was revealed by 1 H and 13 C nuclear magnetic resonance (NMR) spectra respectively, confirming the red pigment produced to be prodigiosin. The molecular weight of the prodigiosin was identified as 323 (m/z 324, [M + H] +). Serratia species that typically develop antibiotic resistance mechanisms are already known to produce antibiotics (carbapenem and bacteriocins) and prodigiosin pigment by integrating Quorum Sensing (QS) with overlapping pigment production regulatory systems (pigP). Consequently, mutations in this regulatory region may influence the production of prodigiosin and antibiotic resistance mechanisms.

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Section: Bacteriological Analysismentioning

confidence: 99%

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

Halder

Banerjee

Biswas

et al. 2020

Environmental Sustainability

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“…L exhibits an intra‐molecular hydrogen bond between phenol ‐OH and the ‐CH=N nitrogen leading to excited state intra‐molecular proton transfer (ESIPT). This ESIPT along with ‐CH=N isomerization are responsible for its weak fluorescence.…”

Section: Resultsmentioning

confidence: 99%

ESIPT‐Based Nanomolar Zn²⁺ Sensor for Human Breast Cancer Cell (MCF7) Imaging

Ghosh

et al. 2017

ChemistrySelect

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A single crystal X‐ray structurally characterized probe (L), derived from 2‐amino‐1‐phenylpropan‐1‐ol and 2‐hydroxy‐1‐napthaldehyde selectively recognizes nano‐molar Zn2+ both by fluorescence and colorimetric method. Inhibition of excited‐state intra‐molecular proton transfer (ESIPT) and ‐CH=N isomerization along with chelation‐enhanced fluorescence (CHEF) collectively contribute to Zn2+ recognition. L exhibits excellent sensitivity (2×10−9 M) and selectivity for Zn2+ in aqueous DMSO (1:1, v/v) media and useful for intra‐cellular Zn2+ imaging in human breast cancer cells (MCF7).

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“…Preliminary morphological analysis of PFAB2 was performed using a Leica DM1000 fluorescence microscope at 100× magnification by incubating the isolate in 1 mM zinc acetate solution followed by binding with a Zn ligand [15]. Morphological and physiological characterization was performed according to the standard protocols.…”

Section: Bacteriological Analysismentioning

confidence: 99%

Molecular and Genomic Characterization of PFAB2: A Non-virulent Bacillus anthracis Strain Isolated from an Indian Hot Spring

Banerjee

Somani

Chakraborty

et al. 2020

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Background: Thermophilic bacilli in both aerobic or facultative anaerobic forms have been isolated for over a hundred years from different mesophilic or thermophilic environments as they are potential source of bioactive secondary metabolites. But the taxonomic resolution in the Bacillus genus at species or at strain level is very challenging for the insufficient divergence of the 16S rRNA genes. One such recurring problem is among Bacillus anthracis, B. cereus and B. thuringiensis. The disease-causing B. anthracis strains have their characteristic virulence factors coded in two wellknown plasmids, namely pXO1 (toxin genes) and pXO2 (capsule genes). Objective: The present study aimed at the molecular and genomic characterization of a recently reported thermophilic and environmental isolate of B. anthracis, strain PFAB2. Methods: We performed comparative genomics between the PFAB2 genome and different strains of B. anthracis, along with closely related B. cereus strains. Results: The pangenomic analysis suggests that the PFAB2 genome harbors no complete prophage genes. Cluster analysis of Bray-Kurtis similarity resemblance matrix revealed that gene content of PFAB2 is more closely related to other environmental strains of B. anthracis. The secretome analysis and the in vitro and in vivo pathogenesis experiments corroborate the avirulent phenotype of this strain. The most probable explanation for this phenotype is the apparent absence of plasmids harboring genes for capsule biosynthesis and toxins secretion in the draft genome. Additional features of PFAB2 are good spore-forming and germinating capabilities and rapid replication ability. Conclusion: The high replication rate in a wide range of temperatures and culture media, the nonpathogenicity, the good spore forming capability and its genomic similarity to the Ames strain together make PFAB2 an interesting model strain for the study of the pathogenic evolution of B. anthracis.

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Structurally Characterized Zn2+ Selective Ratiometric Fluorescence Probe in 100 % Water for HeLa Cell Imaging: Experimental and Computational Studies

Cited by 16 publications

References 52 publications

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

ESIPT‐Based Nanomolar Zn²⁺ Sensor for Human Breast Cancer Cell (MCF7) Imaging

Molecular and Genomic Characterization of PFAB2: A Non-virulent Bacillus anthracis Strain Isolated from an Indian Hot Spring

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Structurally Characterized Zn2+ Selective Ratiometric Fluorescence Probe in 100 % Water for HeLa Cell Imaging: Experimental and Computational Studies

Cited by 16 publications

References 52 publications

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

Production of prodigiosin by a drug-resistant Serratia rubidaea HB01 isolated from sewage

ESIPT‐Based Nanomolar Zn2+ Sensor for Human Breast Cancer Cell (MCF7) Imaging

Molecular and Genomic Characterization of PFAB2: A Non-virulent Bacillus anthracis Strain Isolated from an Indian Hot Spring

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ESIPT‐Based Nanomolar Zn²⁺ Sensor for Human Breast Cancer Cell (MCF7) Imaging