1989
DOI: 10.1016/s0021-9258(18)63857-8
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Structural Organization of the Gene for the E1α Subunit of the Human Pyruvate Dehydrogenase Complex

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Cited by 50 publications
(7 citation statements)
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“…1993 American Chemical Society amplification in the presence of specific primers. The sense primer spanned bases -763 to -743 (5'-CCCCCTTAAAAT-GTGGAGCCC-3') from the putative transcription start site of the human Eia gene (Maragos et al, 1989). Antisense primer (5'-CTTCAGGTGCCCCAGCAGA-3') was complementary to bases +15 to +33 (Maragos et al, 1989) of the human Eia gene.…”
Section: Methodsmentioning
confidence: 99%
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“…1993 American Chemical Society amplification in the presence of specific primers. The sense primer spanned bases -763 to -743 (5'-CCCCCTTAAAAT-GTGGAGCCC-3') from the putative transcription start site of the human Eia gene (Maragos et al, 1989). Antisense primer (5'-CTTCAGGTGCCCCAGCAGA-3') was complementary to bases +15 to +33 (Maragos et al, 1989) of the human Eia gene.…”
Section: Methodsmentioning
confidence: 99%
“…The sense primer spanned bases -763 to -743 (5'-CCCCCTTAAAAT-GTGGAGCCC-3') from the putative transcription start site of the human Eia gene (Maragos et al, 1989). Antisense primer (5'-CTTCAGGTGCCCCAGCAGA-3') was complementary to bases +15 to +33 (Maragos et al, 1989) of the human Eia gene. The amplification mixture (100 ^L) contained 2.5 units of Thermus aquaticus (Taq) polymerase, 100 pmol each of the sense and antisense primers, 200 nmol of each dNTP, and 3 pg of genomic DNA.…”
Section: Methodsmentioning
confidence: 99%
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“…The Ei enzyme is a heterotetramer of 2a and 2(3subunits, but so far only mutations in the E{a gene have been found. This gene is approximately 17 kilobase (kb) long, contains 11 exons, and is localized on the X-chromosome in band Xp22.1 (Koike et al, 1990;Maragos et al, 1989;Brown et alM 1989), mRNA is expressed in all tissues and is approximately 1.5 kb long, encoding a protein of 390 amino acids (Dahl et al, 1987;De Meirleir et al, 1988;Koike et al, 1988;Ho et ah, 1989).…”
Section: Introductionmentioning
confidence: 99%
“…
Ho & Patel, 1990). The DNA sequences of the human Ela and El/3 genes have also been reported (Maragos et al, 1989;Koike et al, 1990). The protein-binding regions and functional cis elements in the 5'-flanking region of the a subunit gene have been reported (Chang et al, 1993), but such information is not available on the human El/3 promoter-regulatory region.
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mentioning
confidence: 99%