1993
DOI: 10.1021/bi00067a014
|View full text |Cite
|
Sign up to set email alerts
|

Multiple protein-binding domains and functional cis-elements in the 5'-flanking region of the human pyruvate dehydrogenase .alpha.-subunit gene

Abstract: We have characterized the 5'-flanking region of the alpha-subunit gene of the human pyruvate dehydrogenase (E1). DNase I footprinting with rat liver nuclear extracts identified 7 major protein-binding domains termed P1 through P7 in a 796 base pair DNA fragment (base pairs -763 to +33). P1 through P4 are clustered in the -221/+33 region. These protein-binding domains contain several known consensus sequences such as a TATA box, CAAT box, Sp1, and CRE, which all have previously been implicated in the constituti… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
8
0

Year Published

1995
1995
2003
2003

Publication Types

Select...
7
1

Relationship

2
6

Authors

Journals

citations
Cited by 16 publications
(8 citation statements)
references
References 29 publications
(37 reference statements)
0
8
0
Order By: Relevance
“…The Elas gene could belong to the rapidly expanding family of genes that are regulated by specific nutrients, although a role for insulin cannot be ruled out at this stage [36,37]. The recent characterization of its promoter [38] should allow us to investigate what factors and DNA elements are involved in this control.…”
Section: Discussionmentioning
confidence: 99%
“…The Elas gene could belong to the rapidly expanding family of genes that are regulated by specific nutrients, although a role for insulin cannot be ruled out at this stage [36,37]. The recent characterization of its promoter [38] should allow us to investigate what factors and DNA elements are involved in this control.…”
Section: Discussionmentioning
confidence: 99%
“…Here we report that thioltransferase can reactivate oxidized NFI protein in vitro in a GSH-dependent manner. In addition, we demonstrate that treatment with buthionine sulfoximine, a known inhibitor of GSH synthesis (24), potentiates the oxidative inactivation of NFI in cultured HeLa cells and that inclusion of N-acetylcysteine (precursor of intracellular GSH) in the culture medium during the recovery period following diamide treatment increases the extent of restoration of the DNA-binding activity of NFI. These findings suggest an important role for thioltransferase and intracellular GSH in the reduction of oxidation-sensitive cysteine residues in the NFI family of transcription factors and potentially other site-specific DNA-binding proteins.…”
mentioning
confidence: 83%
“…Subsequent recovery of the cells was performed for 2 h at 37°C in the presence or absence of the protein synthesis inhibitor cycloheximide (25 g/ml). To deplete the cells of glutathione, cells were incubated with 100 M buthionine sulfoximine (BSO) for 48 h. BSO blocks the synthesis of glutathione by inhibiting the enzyme ␥-glutamylcysteine synthetase (24). More than 90% of the cells were viable under this condition.…”
Section: Sds-polyacrylamide Gel Electrophoresis Andmentioning
confidence: 99%
See 1 more Smart Citation
“…The 5.8 kb (k5.8 kb to j33 bp ; J. Tan and M. S. Patel, unpublished work) or the 796 bp (k763 to j33 bp) [25] human E1α promoter was inserted upstream of the chloramphenicol acetyltransferase (CAT) structure gene in pBluescript (Stratagene, but the β-galactosidase gene in the plasmid was deleted). Deletion constructs were generated by using restriction enzyme digestion at AccI, ApaI and StuI sites of the k763 bp promoter.…”
Section: Construction Of Plasmids and Site-directed Mutagenesismentioning
confidence: 99%