1995
DOI: 10.1021/bi00004a023
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Characterization of the Promoter Regulatory Region of the Human Pyruvate Dehydrogenase .beta. Gene

Abstract: A genomic clone (19 kb) harboring the intron-exon sequences and the promoter-regulatory region of the E1 beta gene of human pyruvate dehydrogenase complex was isolated by screening a placental genomic library. The nucleotide sequence of the promoter region (1245 bp) showed 18 differences (including mismatches, insertions, and deletions) as compared to the published sequence [Koike et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 5594-5597]. The E1 beta promoter lacked a TATA box homology but contained initiator… Show more

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Cited by 14 publications
(6 citation statements)
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“…A 25-fold level of stimulation was observed in K562 cells when the enhancer was located upstream of the heterologous tk promoter and oriented in the same direction as the promoter but only 4-fold in the reverse orientation. Other enhancers have been reported where the extent of functional activity is dependent upon the orientation of the enhancer in the DNA construct (37)(38).…”
Section: Discussionmentioning
confidence: 99%
“…A 25-fold level of stimulation was observed in K562 cells when the enhancer was located upstream of the heterologous tk promoter and oriented in the same direction as the promoter but only 4-fold in the reverse orientation. Other enhancers have been reported where the extent of functional activity is dependent upon the orientation of the enhancer in the DNA construct (37)(38).…”
Section: Discussionmentioning
confidence: 99%
“…These included one site each for LVa-RS, GRuteroglobin, H4TF-1hist, IE1.2 and two sites for WAP-US6. Some of these sites, such as the H4TF-1, have also been found in the promoter regions of other genes [51,52]. However, it is not known if any of these have functional relevance in the H genes.…”
Section: Discussionmentioning
confidence: 99%
“…The only sequence homology in this region to known transcription factor binding sites was to the histone 4 element. Although the H4TF-1 binding site was first identified in the histone H4 gene [36], it has also been found in the human eosinophil peroxidase gene [46] and the human pyruvate dehydrogenase gene [47]. Whether the same nuclear factor is binding to all three sites in these three genes or a different factor for each gene is binding to the same sequence is unknown.…”
Section: Discussionmentioning
confidence: 99%