Structural effects on Cattleya xanthina leaves cultivated in vitro and acclimatized ex vitro

Lando, Ana Paula; Wolfart, Marcia; Fermino, Paulo César Poeta; Santos, Marisa

doi:10.1007/s10535-016-0589-3

Cited by 14 publications

(14 citation statements)

References 30 publications

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“…(2004). A similar result was reported for Cattleya xanthina in which the cells in the mesophyll in the in vitro condition were homogeneous with chlorenchyma, but heterogeneous under ex vitro conditions during the acclimatization process(LANDO et al, 2016). One of the common characteristics in the mesophyll of orchid species is the presence of idioblasts with raphide crystals, which are frequently found in C. nobilior as noted by Zanega-Godoy and Costa (2003) and which were also found in the present study.…”

supporting

confidence: 92%

In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species

Oliveira¹,

Silva

Oliveira

et al. 2021

Div Journ

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The objective of the present study was to evaluate the effects of different culture media on the in vitro germination and initial development of Cattleya nobilior, and to acclimatize this species using soybean straw as a substrate component, aiming the production of plants at large scale to reintroduce it in natural environments. Anatomical characteristics associated with development during acclimatization are also depicted. The influence of Murashige and Skoog, Knudson, and Vacin and Went culture media on the in vitro germination and protocorm development were assessed. Acclimatization was accomplished using different proportions of soybean straw (SS) and Bioplant (BP) as substrates. Anatomical studies were conducted on the leaves of plants during acclimatization. KC was the most suitable culture medium for both germination and initial development of C. nobilior. Regarding acclimatization, it is recommended that a substrate composed of 60% SR and 40% BP be initially used. Upon transfer to shade-house conditions, plants should be grown in a mix of 40% SR: and 60% BP. The leaf anatomical characteristics observed are typical of epiphytic orchids adapted to water-poor environments, which indicates that SR did not negatively affect the development of the species. The in vitro propagation of C. nobilior as described herein is efficient for its multiplication for commercial and conservation purposes and SS can be used as an alternative component of the substrate for its acclimatization. KEYWORDS: Foliar anatomy, culture media, soybean straw.

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supporting

confidence: 92%

In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species

Oliveira¹,

Silva

Oliveira

et al. 2021

Div Journ

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“…The plantlet from in vitro have a special growing condition, heterotrophic metabolisms, such as diminished gas exchange, high air humidity, low irradiance, and use of sugar as carbon and energy source, which could cause inhibition of photosynthesis, abnormal stomata structure, and generally could lead high plantlet mortality during acclimatization. In vitro leaves may not develop a waxy cuticle and functional stomata to the same extent as found in greenhouse leaves (Pospisilova et al 1999;Lesar et al 2012;Lando et al 2016). The stomata rhythms, morphology, and distribution based on the pathways of carbon fixation and light requirements for deacidification.…”

Section: Resultsmentioning

confidence: 98%

“…The significance of environmental differences made a successful transfer from in vitro to ex vitro (greenhouse) conditions is difficult. There are numerous studies on in vitro propagation of orchids that have been published (Arditti 1996;Pospisilova et al 1999;Jawan et al 2010;Lesar et al 2012;Lando et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

Garvita

Wawangningrum

2020

Biodiversitas

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Abstract. Garvita RV, Wawangningrum H. 2020. Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition. Biodiversitas 21: 1116-1121. In vitro micropropagation is an efficient biotechnological strategy for conservation and commercial plantlet production. The ultimate success of in vitro microporapagation depends on the ability to transfer plants out of culture, known as acclimatization stage. The morphology, anatomy and physiology of plantlets in in vitro culture conditions is different from the plants in environment condition (greenhouse), therefore they are unable to compete with soil microbes and to cope with the environmental conditions. The aim of this research is to determine stomata morphology and distribution of Paraphalaenopsis spp (Orchidaceae) from in vitro growth and greenhouse conditions. The leaf stomata cells of Paraphalaenopsis spp. (Paraphalaenopsis serpentilingua, Paraphalaenopsis labukensis and Paraphalaenopsis laycockii) was undertaken by observing the stomata preparation of the upper leaf and lower leaf by using microscope fitted with optic visual seven. The descriptive method was used to describe the stomata morphology by observing the epidermis of longitudinal section and stomata length of upper and lower leaf by using a microscope. The results showed that these plants had anomocytic type of stomata cells. The distributions of stomata are in the upper and lower epidermis leaf in all Paraphalaenopsis spp from in vitro growth and greenhouse.

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“…In vitro environmental conditions employed during plant growth and tissue differentiation were extremely different in terms of light regimes, growth substrates, and other external conditions employed during photomixotrophic hardening in the greenhouse (Chandra et al, 2010;Saez et al, 2012). Structural developments and associated photochemical reactions under ex vitro conditions favor the acclimatization of micropropagated plantlets (Lando et al, 2016). The natural ventilation, increased irradiance, and reduced humidity improved the vegetative anatomy of micropropagated V. negundo under photoautotrophic conditions.…”

Section: Structural Repairs In the Stem Of V Negundomentioning

confidence: 99%

Micro-Structural Stability of Micropropagated Plants ofVitex negundoL.

2021

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Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

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Structural effects on Cattleya xanthina leaves cultivated in vitro and acclimatized ex vitro

Cited by 14 publications

References 30 publications

In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species

In vitro germination, initial development and acclimatization of Cattleya nobilior Rchb. f. (Orchidaceae): an approach to curb the eventual endangerment of this exuberant, near-threatened Cerrado species

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

Micro-Structural Stability of Micropropagated Plants ofVitex negundoL.

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