Micro-Structural Stability of Micropropagated Plants ofVitex negundoL.

Abstract

Micropropagation techniques allow producing large numbers of clones of genetically identical plants. However, there is evidence of disorders in internal structures due to sophisticated in vitro conditions. Such variations are responsible for the mortality of plantlets in the field and cause huge loss to the tissue culture industry. Anatomical evaluation at different growth conditions allows for understanding structural repair of in vitro raised plantlets. Therefore, the present study was aimed to identify the structural changes that occurred in micropropagated plants of Vitex negundo under heterotrophic, photomixotrophic, and photoautotrophic conditions. To achieve this, structural variations were analyzed in the plantlets obtained from in vitro, greenhouse and field transferred stages using light microscopy. Underdeveloped dermal tissues, palisade cells, intercellular spaces, mechanical tissues, vascular bundles, and ground tissues were observed with the plants growing under in vitro conditions. The self-repairing of structural disorders and transitions in vegetative anatomy was observed during hardening under the greenhouse environment. Field transferred plantlets were characterized by well-developed internal anatomy. These findings showed that the micropropagated plantlets of V. negundo were well-adapted through a series of self-repairing the in vitro induced structural abnormalities at the subsequent stages of plant development.

An in vitro Propagation of Aspilia africana (Pers.) C. D. Adams, and Evaluation of Its Anatomy and Physiology of Acclimatized Plants

“…In vitro propagation may result in physiological and anatomical features intrinsic to the media culture environment ( Manokari et al,2021a,b ). Morpho-anatomical, molecular, and biochemical attributes have been used to monitor the stability and integrity of in vitro regenerated plants ( Sonibare and Adeniran, 2014 ).…”

“…Morpho-anatomical, molecular, and biochemical attributes have been used to monitor the stability and integrity of in vitro regenerated plants ( Sonibare and Adeniran, 2014 ). Anatomical features are very important and play key roles in plant identification ( Manokari et al,2021a,b ). We examined and compared the detailed anatomical features of the leaves, stems, and roots of in vitro regenerated A. africana plants and their maternal stocks.…”

Micro‐morpho‐anatomical transitions at various stages of in vitro development of Crinum malabaricum Lekhak and Yadav: A critically endangered medicinal plant

“…2017; Manokari et al . 2021a). One of the major challenges is increased mortality of plants after ex vitro transfer due to underdeveloped and non‐functional cuticles, stomata, mesophyll and vascular elements (Shekhawat et al .…”

“…2017; Manokari et al . 2021a). Therefore, it is essential to develop and transplant plantlets with properly developed structural parameters (Isah 2015; Poniewozik et al .…”

“…Therefore, a better understanding of the complex effects of physiochemical factors on plant micro‐morpho‐anatomical development during in vitro and ex vitro plantlet formation will result in successful survival of plants under field conditions (Manokari et al . 2021a; Shekhawat et al . 2021a, 2021b).…”