Abstract. Garvita RV, Wawangningrum H. 2020. Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition. Biodiversitas 21: 1116-1121. In vitro micropropagation is an efficient biotechnological strategy for conservation and commercial plantlet production. The ultimate success of in vitro microporapagation depends on the ability to transfer plants out of culture, known as acclimatization stage. The morphology, anatomy and physiology of plantlets in in vitro culture conditions is different from the plants in environment condition (greenhouse), therefore they are unable to compete with soil microbes and to cope with the environmental conditions. The aim of this research is to determine stomata morphology and distribution of Paraphalaenopsis spp (Orchidaceae) from in vitro growth and greenhouse conditions. The leaf stomata cells of Paraphalaenopsis spp. (Paraphalaenopsis serpentilingua, Paraphalaenopsis labukensis and Paraphalaenopsis laycockii) was undertaken by observing the stomata preparation of the upper leaf and lower leaf by using microscope fitted with optic visual seven. The descriptive method was used to describe the stomata morphology by observing the epidermis of longitudinal section and stomata length of upper and lower leaf by using a microscope. The results showed that these plants had anomocytic type of stomata cells. The distributions of stomata are in the upper and lower epidermis leaf in all Paraphalaenopsis spp from in vitro growth and greenhouse.
Hoya purpureofusca Hook.f. (Apocynaceae: Asclepiadoideae) has been used as an ornamental plant and the international trade of this species has become increasing. This species has restricted distribution on the high elevation of Java and Bali mountains. This epiphyte climber has succulent leaves and umbellate delicate flowers. Flower has star shape, succulent and waxy, purple., c.1 cm in diameter. The observation on the morphological characteristic is aimed to select the best sample for ornamental plant. The total of 17 observed samples were obtained from three populations at Gunung Gede Pangrango National Park, West Java, Indonesia. The selection was based on the node length, size of the leaves, and flower number, size and color. The result showed that P01 from Cibodas could be developed as leaf ornamental plant, because its shortest node and small leaves. SP1 from Selabintana could be developed as flower ornamental plant, it has numerous, larger and deep purple flower. SP1 and P01 have the farest relatives distance among all observed accessions, but still have a similarity of 75%.
Aeschynanthus (Gesneriaceae) is climbing or trailing epiphytes from the tropical forests of South-East Asia. Several species are widely cultivated for their attractive flowers, which usually scarlet with long corolla tubes and probably bird pollinated.This study was carried out on Aeschynanthus tricolor Hook. to determine in vitro germinability of (1) pollen collected from flowers at anthesis, on the day before anthesis, or up to five days after anthesis and (2) pollen collected on the day of anthesis and then stored at -20 o C for 127 days. Germinability tests were carried out on pollen grains after stored for four hours in 10% sucrose solution. The highest in vitro germination percentage observed was pollen collected on the day of anthesis (96.3%) and the lowest was pollen collected on the fifth day after anthesis (5.6%). The germination percentage of pollen after stored at -20 o C was 98% at beginning and 22% after 127 days. From this research we can conclude that pollen of A. tricolor can be successfully collected and then stored for some time, will be practical benefit for plant breeding and conservation purposes.
Nepenthes is one of carnivorous plants which very popular as ornamental plant. Most of them grow in mountain forest habitat above 1.000 m a.s.l. Sumatra has the richest Nepenthes flora after Borneo, with 29 species. The observation was done in Sulasih Talang Nature Reserve -West Sumatra. According to this inventory in Sulasih Talang Nature Reserve, there are 6 species can be found in this area, which 5 species are endemic to Sumatra, that are N. pectinata, N. inermis, N. bongso, N. spathulata and N. talangensis. N. gracilis is widespread in Sumatra, Borneo and Celebes, whereas the other species N. talangensis is only found in Mount Talang. Threat to those species are, habitat destruction, over exploitation and volcano eruption.
Sulasih Talang Nature Reserve is a conservation area in West Sumatra Province, which contains high diversity of plants including Araliaceae. Araliaceae mostly consist of attractive plants which have potentiallity as ornamental plants. The aim of this research is to record the diversity of Araliaceae in Sulasih Talang Nature Reserve. The result of the study indicates that there are, species of Araliaceae found in
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