Structural Diversity of Class 1 Integrons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospital in China

Li, Bin; Hu, Yongfei; Wang, Qi; Yao, Yi; Woo, Patrick C. Y.; Hua, Jing; Zhu, Baoli; Liu, Cui Hua

doi:10.1371/journal.pone.0075805

Cited by 52 publications

(48 citation statements)

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“…In our study, the frequency of ceftriaxone resistance was 31%, which is higher than the rates reported in studies in the Netherlands (18.7%) and in the USA (10%) (27,28). The inconsistency in the antibiotic resistance rates might be due to differences in geographical area, type of antibiotics used, type of samples and indiscriminate use of antibiotics (29,30).…”

Section: Discussioncontrasting

confidence: 76%

Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

et al. 2018

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Background and Objectives: The resistance of gram-negative bacteria to antibiotics has become a serious problem, which imposes a significant increase in treatment costs. Klebsiella pneumoniae is an important nosocomial pathogen from the Enterobacteriaceae family. The aim of this study was to investigate the frequency and pattern of antibiotic resistance in K. pneumoniae strains isolated from clinical samples.Methods: This descriptive, cross-sectional study was performed on 150 K. pneumonia strains isolated from different clinical samples such as urine, sputum, blood, ulcers, lung secretions and abdominal abscess. Antibiogram test was performed using the disk diffusion method (Kirby-Bauer). Minimum inhibitory concentration of amikacin, tobramycin and gentamicin was determined via the E-test for 50 strains with high resistance rates.Results: In this study, the highest rate of resistance was observed against carbenicilin, ceftriaxone, cefepime and streptomycin. K. pneumonia isolates were most frequent in urine and sputum samples. In the E-test, the highest rate of resistance was observed against gentamicin, tobramycin (16µg/ml) and amikacin (64µg/ml).Conclusion: Based on our results, tigecycline, netilmicin, kanamycin and amikacin are the most effective antibiotics for the treatment of K. pneumoniae infections.

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Section: Discussioncontrasting

confidence: 76%

Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

et al. 2018

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“…22 In our study, integron-mediated resistance genes were obviously associated with aminoglycoside resistance.…”

Section: Discussionmentioning

confidence: 52%

耐碳青霉烯类肺炎克雷白杆菌临床分布及整合素1与其耐药关系的研究

et al. 2017

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Objective The aim of our study is to investigate the prevalence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) and the genetic characteristics of the class 1 integron in CRKP on multi-drug resistance.Methods Clinical Klebsiella pneumoniae strains were collected from multiple departments of a hospital in central China. CRKP strains were identified among the isolates, and antibiotics susceptibility of CRKP strains was analyzed. The polymerase chain reaction (PCR) was adopted to amplify the class 1 integron variable area. The integron genetic structure was analyzed with enzyme digestion and DNA sequencing technology. The relation between class 1 integron and drug resistance was analyzed statistically.Results Totally 955 strains of Klebsiella pneumoniae were isolated from varied sites of the hospital, and 117(12.3%) of them were identified as CRKP, with a separation rate of 8.9% (26/292) in 2013, 11.3% (38/336) in 2014 and 16.2% (53/327) in 2015, which shows an increasing trend by year. 44.4% (52/117) of CRKP strains were separated from specimen of ICU, and 61.5% (72/117) were from sputum. Over 95% CRKP strains were resistant to ampicillin/sulbactam, aztreonam, imipenem, meropenem, ceftazidme, cefotaxime, cefepime, and piperacillin, while relatively low resistant rates were found in tigecycline (12.8%) and colistin (35.9%). The class 1 integron was detected in 77.8% (91/117) of CRKP strains. Class 1 integron of CRKP was significantly correlated with the antibiotic resistance to the tobramycin, gentamicin and amikacin (all P<0.01). The gene cassette analysis of variable area of class 1 integron showed that aadA2 accounts for 64.8% (59/91), aacA4-catB8-aadA1 23.1% (21/91), and aadA2-dfrA25 12.1% (11/91).Conclusions CRKP has an increasing trend in a clinical setting in China, and most of them were resistant to multiple antibiotics. Class 1 integron in CRKP has strong ability to capture the genes resistant to aminoglycosides antibiotics from environment, with the aadA2 gene as the most popular one.

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“…El gen orfF es un gen casete cuyo producto es una proteí-na hipotética, con función desconocida (Li et al 2013). …”

Section: Discussionunclassified

“…Ambos pertenecen a la familia de los antibióticos aminoglucósidos. La estreptomicina es de gran importancia en el tratamiento de Mycobacterium tuberculosis y la espectinomicina es empleada en el tratamiento de la gonorrea (Li et al 2013).…”

Section: Discussionunclassified

“…Klebsiella pneumoniae productora de carabpenemasa (KPC) es un grupo de bacterias emergentes altamente resistentes a antibióticos comúnmente empleados en el tratamiento de infecciones graves producidas por bacilos Gram negativos (Li et al 2013). Las infecciones producidas por este tipo de aislados representan un reto terapéutico, sobre todo en el tratamiento empírico inicial.…”

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Caracterización de la región variable de integrones clase 1 en aislados clínicos de Klebsiella pneumoniae resistentes a carbapenemes

Yauri¹,

Alcocer²,

Rodríguez-Riglos³

2016

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RESUMEN.-Los integrones son estructuras observadas con frecuencia en bacilos Gram negativos, especialmente entre los miembros de la familia Enterobacteriaceae. Se estudió la presencia de integrones clase 1 y su relación con la resistencia bacteriana. La presencia de integrones fue estudiada por medio de la reacción en cadena de la polimerasa (PCR) y secuenciación. Se analizaron treinta aislados resistentes a carbapenemes de Klebsiella pneumoniae que fueron positivos para el gen de la integrasa 1 (intI1). En veintiseis de estos aislados se amplificaron regiones variables de alrededor de 2 000 pares de bases. El patrón común de genes obtenidos mediante el análisis de estas regiones fue (dfrA12 -orfF -aadA2). La presencia de estas plataformas que promueven la diversidad genética provee a las bacterias de resistencia a una amplia gama de antibióticos. La alta prevalencia de integrones observadas entre estos aislados funciona como fuente de difusión de determinantes de resistencia. PALABRAS CLAVES:carbapenemes,genes casete, integrones clase 1, Klebsiella pneumoniae, resistencia antibió-tica.ABSTRACT.-Integrons are structures frequently observed in Gram negative bacilli, especially among members of the Enterobacteriaceae family. The presence of class 1 integron and the relationship with bacterial resistance were studied. The integron presence was investigated by polymerase chain reaction (PCR) and sequencing. Thirty carbapenem-resistant Klebsiella pneumoniae isolates were analyzed and were positive for the intI1 gene. Twenty six of these isolates amplified variable regions about 2000 base pairs. The common pattern of genes was (dfrA12-OrfF-aadA2). The presence of these platforms that promote genetic diversity gives resistance a broad range of antibiotics. The higher integron prevalence in this isolates functions as source for dissemination of resistance determinants.

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Structural Diversity of Class 1 Integrons and Their Associated Gene Cassettes in Klebsiella pneumoniae Isolates from a Hospital in China

Cited by 52 publications

References 51 publications

Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

耐碳青霉烯类肺炎克雷白杆菌临床分布及整合素1与其耐药关系的研究

Caracterización de la región variable de integrones clase 1 en aislados clínicos de Klebsiella pneumoniae resistentes a carbapenemes

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