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The antimicrobial susceptibility of 212 Salmonella strains isolated from patients and foods was evaluated and 45% were found to be resistant to nalidixic acid. Nalidixic acid resistant strains showed a higher minimal inhibitory concentration for ciprofloxacin than sensitive strains. During the study an increase of strains with reduced susceptibility to ciprofloxacin was also observed.

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RESUMOSalmonelose é a infecção bacteriana de origem alimentar mais freqüente no Paraná, Brasil, e os surtos estão associados, principalmente, ao consumo de ovos, carne de aves e derivados. Os objetivos deste trabalho foram identificar os sorovares de Salmonella isolados de carcaças de frango e caracterizá-los molecularmente por rEP e ErIc-Pcr, assim como identificar os fagotipos de Salmonella Enteriditis. Dos 25 isolados de Salmonella spp. analisados, 18 foram identificados como Enteriditis, 4 como Braenderup, 2 como Worthington e 1 como infantis. Dos 18 isolados de Enteriditis, 14 foram PT4, 2 PT4a, 1 PT7 e 1 rDNc, por se tratar de colônia rugosa. rEP-Pcr forneceu padrão eletroforético distinto de 10 a 13 bandas distribuídas entre 120 e 2072 pb para cada sorovar diferente testado. A ErIc-Pcr mostrou um padrão de 4 a 5 bandas entre 180 e 1000 pb e foi menos discriminativa quando comparada à rEP-Pcr. Os resultados encontrados confirmaram que a fagotipagem é uma ferramenta útil e discriminativa para o sorovar Enteriditis. Apesar do pequeno número de sorovares testados, os resultados sugerem que a rEP-Pcr parece ser um método atrativo a ser utilizado no futuro para a discriminação preliminar de sorovares de Salmonella. Palavras-chaves: Salmonella Enteriditis, fagotipos, sorovar, rEP-Pcr, ErIc-Pcr. SUMMARYDIScrIMINATION OF Salmonella SErOVArS ISOlATED FrOM cHIcKEN MEAT BY rEP AND ErIc-Pcr AND PHAGOTYPING OF Enteriditis SOrOVAr. Salmonellosis is the most prevalent bacterial food-borne disease in the State of Paraná, Brazil, and the outbreaks are often associated with consumption of poultry products. The aim of this study was to serotype Salmonella strains isolated from chicken carcasses and characterize them molecularly using rEP and ErIc-Pcr. The phage types of Salmonella Enteriditis were also identified. Of the 25 Salmonella strains analysed, 18 were identified as Enteriditis, 4 as Braenderup, 2 as Worthington and 1 as infantis. Of the 18 Enteriditis isolates, 14 were PT4, 2 PT4a, 1 PT7 and 1 "reacted, but did not conform" -rDNc. Distinct rEP-Pcr profiles with 10 to 13 fragments distributed between 120 and 2072 pb were easily obtained for each serovar tested. ErIc-Pcr showed patterns of 4 to 5 fragments between 180 and 1000 pb and less discriminatory power than rEPPcr. The results confirmed that phage typing is a useful tool to differentiate into sorovar Enteriditis. Although it is recognized that only a limited number of strains was tested in this study, the results suggest that rEP-Pcr could be an attractive choice to be used in the future as a preliminary method of Salmonella sorovar discrimination.