Structural characterization of fucose-containing disaccharides prepared from exopolysaccharides of Enterobacter sakazakii

Xiao, Mengshi; Fu, Xiaodan; Wei, Xinyi; Chi, Yongzhou; Gao, Wei; Yu, Ying; Liu, Zhemin; Zhu, Chunshen; Mou, Haijin

doi:10.1016/j.carbpol.2020.117139

Cited by 15 publications

(8 citation statements)

References 34 publications

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“…98, 75.73, 71.15, and 72.06 from HSQC spectrum (Figure 5D). According to the relevant literature (Xiao et al, 2021), residue F was considered as β-L-Fucp-(1→.…”

Section: Glycosidic Linkage Analysismentioning

confidence: 99%

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

et al. 2022

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Pleurotus placentodes, a fungus, belongs to the Pleurotaceae family. The aim of the present study was to characterize the structure of a novel polysaccharide from fruiting bodies of P. placentodes (PPp-W) and evaluate its anticoagulant activity in vitro. The high-performance liquid chromatography and GC–MS analysis indicated that PPp-W with a molecular weight of 27.4 kDa was mainly composed of mannose (17.56%), glucose (6.37%), galactose (44.89%), and fucose (1.22%) with a certain amount of 3-O-methyled galactose. SEM, XRD, and AFM combined with Congo red test revealed that PPp-W was an irregular curly sheet with triple-helix conformation. The FT-IR, methylation, and nuclear magnetic resonance analysis indicated that PPp-W contained→6)-α-D-Galp-(1→, →6)-3-O-Me-α-D-Galp-(1→and →2, 6)-α-D-Galp-(1→ as main chain, partially substituted at O-2 and O-6 by non-reducing ends of β-D-Manp-(1→ and β-L-Fucp-(1→ with a small amount of α-1,3-linked-Glcp in backbone. PPp-W could significantly prolong APTT (12.9 ± 0.42 s, p < 0.001) and thrombin time (39.9 ± 0.28 s, p < 0.01) compared with the control group (11.45 ± 0.071 s and 38.05 ± 0.21 s), which showed that PPp-W had anticoagulant activity. These studies suggested that PPp-W was a 3-O-methylated heteroglycan and might be suitable for functional foods and natural drugs as an anticoagulant ingredient, which provided a basis for the application of polysaccharides from P. placentodes.

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“…98, 75.73, 71.15, and 72.06 from HSQC spectrum (Figure 5D). According to the relevant literature (Xiao et al, 2021), residue F was considered as β-L-Fucp-(1→.…”

Section: Glycosidic Linkage Analysismentioning

confidence: 99%

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

et al. 2022

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“…Bacteriophage-borne glycanases extracted from phages are effective tools for degrading FcEPSs [8]. A previous study found that a phage isolated from sewage was capable of degrading FcEPS produced by C. sakazakii M1 [9]. In this study, the bacteriophage targeting C. sakazakii M1 was purified via a plaque assay, more than 10 times.…”

Section: Main Textmentioning

confidence: 99%

“…Phage isolation and purification were performed as described previously [9]. Briefly, prior to phage PF-CE2 DNA isolation, DNase I (10 µg/mL, Sigma-Aldrich) and RNase A (20 µg/mL, Sigma-Aldrich) were added to a purified suspension of PF-CE2 and incubated at 37°C for 1 h, to digest bacterial DNA and RNA.…”

Section: Main Textmentioning

confidence: 99%

Genome Sequence Analysis of Cronobacter Phage PF-CE2 and Proposal of a New Species in the Escherichia Virus RB16 Genus

Xiao

Ren

et al. 2021

Preprint

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The genome of Cronobacter sakazakii M1 phage named PF-CE2 was characterized in this work. And a new species named Cronobacter virus PF-CE2, in the Escherichia virus RB16 genus of the subfamily Tevenvirinae of the family Myoviridae was established. The Gp190 gene of phage PF-CE2 was first proposed to encode a bacteriophage-borne glycanase, which is capable of degrading fucose-containing exopolysaccharides produced by C. sakazakii M1. Further, the taxonomic status of eight additional phages was modified according to average nucleotide identity analysis. This finding provides a theoretical basis for subsequent heterologous expression of the phage PF-CE2 glycanase and provides an important reference for the preservation and sharing of these phages.

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“…However, the complexity and high cost of fucose chemical synthesis make it unable to meet the demands of large-scale industrial production. Recent years, fucose-containing polysaccharides (FCPs) and fucose-containing oligosaccharides (FCOs) have attracted more and more attentions owing to their abundant sources (plants, sea animals, and microorganisms) and functional activities ( Freitas et al, 2011 , Xiao et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Microbial exopolysaccharides (EPS) are promising sources of fucose, FCOs and FCPs. EPS are high molecular weight carbohydrate polymers produced by many microorganisms, including bacteria, fungi, and microalgae ( Xiao et al, 2021 ). Bacterial EPS can be secreted extracellularly in two different states: capsular polysaccharides that are closely associated with the cell surface and form a capsule or as slime polysaccharides that are loosely attached or even totally secreted into the cell environment ( Barcelos, Vespermann, Pelissari, & Molina, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Fucose-containing bacterial exopolysaccharides: Sources, biological activities, and food applications

Xiao

Ren

et al. 2022

Food Chemistry: X

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Structural characterization of fucose-containing disaccharides prepared from exopolysaccharides of Enterobacter sakazakii

Cited by 15 publications

References 34 publications

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

Structural Characterization and Anticoagulant Activity of a 3-O-Methylated Heteroglycan From Fruiting Bodies of Pleurotus placentodes

Genome Sequence Analysis of Cronobacter Phage PF-CE2 and Proposal of a New Species in the Escherichia Virus RB16 Genus

Fucose-containing bacterial exopolysaccharides: Sources, biological activities, and food applications

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