Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

Guan, Xiong; Qi, Jiayi; Ho, Chi Tang; Li, Bin; Mu, Jingjing; Zhang, Yuting; Hu, Huiping; Mo, Weipeng; Chen, Zhongzheng; Xie, Yizhen

doi:10.1016/j.carbpol.2020.116874

Cited by 86 publications

(59 citation statements)

References 72 publications

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“…Using an ultrafiltration instrument, the water-soluble CGLP was separated into three fractions: 5-10 kDa (CGLP-1), 10-100 kDa (CGLP-2), and >100 kDa (CGLP-3). The structural characteristics and immunomodulatory activity of purified CGLP-3 have been investigated in our previous study [20]. In the current study, to obtain homogenized polysaccharides, both CGLP-1 and CGLP-2 were subjected to a DEAE-Sepharose fast-flow column and eluted with ultrapure water and 0.1-0.5 M NaCl (Figure 1A,B).…”

Section: Purification Component Analysis and Molecular Weights Of Glp-1 And Glp-2mentioning

confidence: 85%

“…leucocontextum fruiting bodies cultured in 2018 were provided by Huiping Hu (Guangdong Institute of Microbiology, Guangzhou, China). The crude G. leucocontextum polysaccharide (CGLP) was obtained through hot-water extraction, ethanol precipitation, deproteinization, dialysis, and lyophilization according to our recent study [20].…”

Section: Extraction Of Crude Polysaccharidementioning

confidence: 99%

“…CGLP was redissolved in ultrapure water and separated into three fractions (CGLP-1: 5-10 kDa, CGLP-2: 10-100 kDa, and CGLP-3: >100 kDa) using the cross-flow ultrafiltration membrane of 100 kDa and 10 kDa MWCO (200 cm 2 , PES; Sartorius, Göttingen, Germany). The structural characterization and immunomodulatory activity of purified CGLP-3 have been investigated in our group [20]. Therefore, CGLP-1 and CGLP-2 were poured into a DEAE-Sepharose fast-flow anion exchange column (2.6 cm × 30 cm) in this study.…”

Section: Fractionation and Purificationmentioning

confidence: 99%

“…The conformational structures of GLP-1 and GLP-2 were determined using the Congo red method described by Gao et al [20].…”

Section: Congo Red Testmentioning

confidence: 99%

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Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Guan

et al. 2021

Antioxidants

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Two low-molecular-weight polysaccharides (GLP-1 and GLP-2) were purified from Ganoderma leucocontextum fruiting bodies, and their physicochemical properties and antioxidant activities were investigated and compared in this study. The results showed that GLP-1 and GLP-2 were mainly composed of mannose, glucose, galactose, xylose, and arabinose, with weight-average molecular weights of 6.31 and 14.07 kDa, respectively. Additionally, GLP-1 and GLP-2 had a similar chain conformation, crystal structure, and molecular surface morphology. Moreover, GLP-1 exhibited stronger antioxidant activities than GLP-2 in five different assays: 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), hydroxyl radical, superoxide anion radical, ferric reducing antioxidant power (FRAP), and oxygen radical antioxidant capacity (ORAC). The main linkage types of GLP-1 were found to be →4)-α-D-Glcp-(1→, →4)-β-D-Glcp-(1→, →3)-β-D-Glcp-(1→, →6)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →4,6)-α-D-Glcp-(1→, and Glcp-(1→ by methylation analysis and nuclear magnetic resonance (NMR) spectroscopy. In addition, GLP-1 could protect NIH3T3 cells against tert-butyl hydroperoxide (tBHP)-induced oxidative damage by increasing catalase (CAT) and glutathione peroxidase (GSH-Px) activities, elevating the glutathione/oxidized glutathione (GSH/GSSG) ratio, and decreasing the malondialdehyde (MDA) level. These findings indicated that GLP-1 could be explored as a potential antioxidant agent for application in functional foods.

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Section: Purification Component Analysis and Molecular Weights Of Glp-1 And Glp-2mentioning

confidence: 85%

Section: Extraction Of Crude Polysaccharidementioning

confidence: 99%

Section: Fractionation and Purificationmentioning

confidence: 99%

“…The conformational structures of GLP-1 and GLP-2 were determined using the Congo red method described by Gao et al [20].…”

Section: Congo Red Testmentioning

confidence: 99%

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Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Guan

et al. 2021

Antioxidants

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“…It can be seen from the figure that the five polysaccharides showed the typical absorption peaks of polysaccharides, and there was no new structural peaks in the spectroscopy, which indicated that the extraction methods had no effect on the chemical structure of polysaccharides. The absorption peak near 3,400 cm -1 was O-H stretching vibration (Ognyanov et al, 2018;Sharma et al, 2020); the absorption peak near 2,940 cm -1 was C-H stretching vibrations and variable-angle vibrations (Gao et al, 2020); the absorption peak near 1,740 cm -1 was C=O stretching vibration, indicating the presence of uronic acid in RPs (Gao et al, 2020);…”

Section: Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 98%

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

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The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

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Isolation and Characterization of trans‐p‐Hydroxycinnamoyl Meroterpenoids from Ganoderma sinensis

Yin

Zhao

Zhang

et al. 2023

Chemistry & Biodiversity

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Zizhines V, W, Y, Z, (�)-zizhines X, and Z1À Z3, and (�)-ganosinensol L, thirteen new compounds including four pairs of enantiomers and a known compound (À )-ganosinensol L, were isolated from the fruiting bodies of Ganoderma sinensis. Their structures were identified by spectroscopic, computational methods, and CD (circular dichroism spectroscopy) comparisons. Zizhines VÀ Z and Z1À Z3 are meroterpenoids consisting of the phenolic and the terpenoidal parts. All the compounds except zizhine Z3 bear a common trans-p-hydroxycinnamoyl group. Biological evaluation shows that (À )-zizhine Z1 inhibits cell migration in the MDA-MB-231 cell lines. The present study discloses the chemical profiling of G. sinensis and paves the way for its development as functional products to benefit chronic disorders.

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Structural characterization and immunomodulatory activity of a water-soluble polysaccharide from Ganoderma leucocontextum fruiting bodies

Cited by 86 publications

References 72 publications

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Purification, Physicochemical Properties, and Antioxidant Activities of Two Low-Molecular-Weight Polysaccharides from Ganoderma leucocontextum Fruiting Bodies

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Isolation and Characterization of trans‐p‐Hydroxycinnamoyl Meroterpenoids from Ganoderma sinensis

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